Targeted Cancer Therapeutics

Hait, William N.

doi:10.1158/0008-5472.can-08-3836

Cited by 141 publications

(101 citation statements)

References 35 publications

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“…Similar to HsA, the intensity of the CD band associated to Tf decreased upon addition of the metallaprisms. Unlike HsA, the intensity reduction was less pronounced, but a significant decrease could be observed with metallaprism [2] 6+ compared to metallaprisms [1] 6+ and [3] 6+ . These observations are consistent with the NMR data, in which the precipitation of Tf was more noticeable with metallaprism [2] 6+ .…”

Section: Nmr and Ms Investigations Of The Interaction Between Metallamentioning

confidence: 85%

“…Surprisingly, neither the mass spectra nor the NMR spectra have indicated covalent interactions between Ub and the metallaprisms. On the other hand, the CD spectra suggest that strong electrostatic interactions take place with the metallaprisms [2] 6+ and [3] 6+ , the CD band being significantly quenched. Moreover, in the presence of [1] 6+ , the negative band of Ub was shifted, while the intensity of the band remained strong (Fig.…”

Section: Organic and Biomolecular Chemistry Papermentioning

confidence: 95%

“…Additionally, the resonances of the protein became gradually broadened, indicating an ongoing reaction or an interaction with the metallaprism [2] 6+ . On the other hand, in the presence of [3] 6+ , Mb precipitated almost completely while the signals of the metallaprism remained sharp and well resolved (Fig. S11 †).…”

Section: Nmr and Ms Investigations Of The Interaction Between Metallamentioning

confidence: 99%

“…This quest for finding truly selective compounds has resulted in what is today known as targeted therapy. 3,4 The increased selectivity represents the most important feature of the newly developed drugs in order to minimize side effects and to prevent the development of resistance mechanisms. 5,6 As emphasized in the literature, in order to successfully design and develop new and selective metal-based drugs, it is of the highest importance to understand the mode of action of established and also of newly developed anticancer drugs.…”

Section: Introductionmentioning

confidence: 99%

“…Our previous work describing the reactivity of the metallaprisms [( p-cymene) 6 [3] 6+ , with in some cases the formation of degradation products, especially in the presence of basic amino acids (Arg, His and Lys). [33][34][35] For this reason, interactions can be expected with proteins, especially with ubiquitin.…”

Section: Introductionmentioning

confidence: 99%

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Interactions of arene ruthenium metallaprisms with human proteins

2015

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Interactions between three hexacationic arene ruthenium metallaprisms, [( p-cymene) 6+ , and a series of human proteins including human serum albumin, transferrin, cytochrome c, myoglobin and ubiquitin have been studied using NMR spectroscopy, mass spectrometry and circular dichroism spectroscopy. All data suggest that no covalent adducts are formed between the proteins and the metallaprisms. Indeed, in most cases electrostatic interactions, leading to precipitation of protein-metallaprism aggregates, have been observed. In addition, with the smallest proteins, ubiquitin, myoglobin and cytochrome c, the presence of the hexacationic arene ruthenium metallaprisms induces structural changes of the proteins, as emphasized by circular dichroism. The results suggest that proteins are certainly a biological target for these metalla-assemblies.

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Section: Nmr and Ms Investigations Of The Interaction Between Metallamentioning

confidence: 85%

Section: Organic and Biomolecular Chemistry Papermentioning

confidence: 95%

Section: Nmr and Ms Investigations Of The Interaction Between Metallamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interactions of arene ruthenium metallaprisms with human proteins

2015

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Fluorescence Imaging

Albani

2011

Encyclopedia of Analytical Chemistry

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Fluorescence Imaging

Albani

2016

Encyclopedia of Analytical Chemistry

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A number of fluorescence imaging techniques show diagnostic promise. Imaging endogenous fluorescence has been proposed as a method for cancer diagnosis. Unfortunately, tissue auto fluorescence is relatively weak and poor contrast between malignant and normal tissue is seen. Contrast may be enhanced with the addition of fluorescent materials that are selectively accumulated by malignant cells, such as fluorescein or porphyrin derivatives. The limited penetration of light at the emission maxima of these materials restricts the use of fluorescence techniques utilizing these chromophores to superficial phenomena. However, many potential applications still exist. For example, monitoring fluorescence during surgery may allow resection margins to be clearly delineated. Other exogenous chromophores that may have diagnostic utility include indocyanine green (ICG). Techniques based on visualization of the distribution of ICG fluorescence (i.e., choroidal angiography) are already prominent in ophthalmology. ICG fluorescence imaging may also find a useful niche in monitoring of burns and transplant tissue. In addition, monitoring of vascular parameters during cardiac surgery presents exciting opportunities. For example, low oxygen levels (e.g., during bypass surgery) in the heart can result in alterations in microvascular permeability. As ICG is largely bound to serum albumin, it should not be seen in extravascular spaces in normal hearts. However, increased permeability will allow albumin to diffuse into the extravascular spaces, and diffuse fluorescence across the surface of the heart will be seen. In principle, the infusion of polymers (e.g., dextrans) of various molecular weights labeled with dyes that fluoresce at various wavelengths will allow the assessment of the porosity of capillary beds in such systems. Immunofluorescence techniques have the potential to provide unmatched sensitivity and specificity. The unique nature of antibody‐antigen interactions ensures specific delivery of the fluorophore to the site of interest. The specific interaction of labeled antibodies with antigens means that the fluorophore persists in the body for a prolonged period of time (days). Following a single injection of labeled antibody, repeated measurements on the same site over the course of hours or days allow kinetic information to be readily obtained. In principle, this means that the effect of therapeutic intervention, i.e., radiation therapy, chemotherapy, and so on, can be monitored.

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Targeted Cancer Therapeutics

Abstract: Targeted therapies can be

Cited by 141 publications

References 35 publications

Interactions of arene ruthenium metallaprisms with human proteins

Interactions of arene ruthenium metallaprisms with human proteins

Fluorescence Imaging

Fluorescence Imaging

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