Interactions of arene ruthenium metallaprisms with human proteins

Abstract

Interactions between three hexacationic arene ruthenium metallaprisms, [( p-cymene) 6+ , and a series of human proteins including human serum albumin, transferrin, cytochrome c, myoglobin and ubiquitin have been studied using NMR spectroscopy, mass spectrometry and circular dichroism spectroscopy. All data suggest that no covalent adducts are formed between the proteins and the metallaprisms. Indeed, in most cases electrostatic interactions, leading to precipitation of protein-metallaprism aggregates, have been observed. In addition, with the smallest proteins, ubiquitin, myoglobin and cytochrome c, the presence of the hexacationic arene ruthenium metallaprisms induces structural changes of the proteins, as emphasized by circular dichroism. The results suggest that proteins are certainly a biological target for these metalla-assemblies.

Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

“…[31,35,36] Similar results were obtained with three hexacationic arene ruthenium metallaprisms and severalh uman proteins.N oc ovalent adducts are formed between the proteins and the metallaprisms, and only electrostatic interactions have been observed. [42] It is likely that DiRu-1r emains intact during encapsulation, as the compound is stable under the experimental conditions used for the encapsulationp rotocol (Supporting Information Figure S4), in agreement with previouss tructural stabilityd ata collected at both acidic and basic pH values. [34] To further confirm the presence of Ru atoms within the crystals of DiRu-1-encapsulated AFt, ICP-MS measurements were performed.…”

“…Similar results were obtained with three hexacationic arene ruthenium metallaprisms and several human proteins. No covalent adducts are formed between the proteins and the metallaprisms, and only electrostatic interactions have been observed …”

Investigation of the Anticancer Activity of Coordination-Driven Self-AssembledTwo-Dimensional Ruthenium Metalla-Rectangle

“…Furthermore, the planarity of urea functionalized bis-pyridyl amide linker and the nature of ruthenium complex 2 further approves the proposed structure of self-assembled metalla-rectangle 3 [64,65,66]. It is worth mentioning that the variation in symmetry elements of organic linkers and ligand to metal complex ratio afford different discrete architectures [67], for instance, self-assembly of pyridine based trigonal planar linker and half-sandwich ruthenium complex in 2:3 ratio led to ruthenium based metalla-prisms [68,69].…”

Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC

“…As part of a study, looking at the development of ruthenium cage molecules based on a 2,4,6-tris(di-2-pyridylamino)-1,3,5-triazine ligand system (Figure 1), we reported on the synthesis of star-shaped trinuclear complexes [13]. These polynuclear ruthenium organometallic compounds were shown to interact with DNA in vitro , and are also effective in binding to human proteins [14]. We had hypothesized that in a mechanism similar to what is usually proposed for mononuclear compounds, the Ru-chloride bond would hydrolyze in vitro to provide a reactive intermediate with a Ru-H 2 O bond [15–17].…”