Targeted and Untargeted Liver Metabolomics Demonstrate Key Differences of Perchloric acid and Acetonitrile Isopropanol Water Extraction Through Gas Chromatography Mass Spectrometry
Abstract:In this study, untargeted metabolic profiling by Gas Chromatography Mass Spectrometry (GC‐MS) was leveraged to identify differences between Perchloric acid extraction (PCA) and 3:3:2 acetonitrile/isopropanol/water (IPA) extraction for liver metabolomics research. Perchloric acid extraction (PCA), a common means of precipitating excess protein through acidification, has been used in physiology literature for decades and is considered as the standard method for extracting water soluble metabolites. However, PCA … Show more
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