“… Fig. 4 Other cell trapping methods based on different physical fields: A DEP-based single cell trapping (Qin et al 2018 ), B optical tweezer-based single cell trapping (Cong et al 2019 ), C magnetic-based single cell trapping (Shields et al 2016 ) …”
Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning
confidence: 99%
“…This platform can achieve selective capture of single cells with light beams (Huang et al 2013 ). For increasing the range of optical trapping, Cong et al ( 2019 ) made a focused optical beam trap by combining optical forces and convective drag forces to guide living single cancer cell into a microwell array for multidrug-resistant gene marker detection (Fig. 4 B).…”
Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning
Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.
“… Fig. 4 Other cell trapping methods based on different physical fields: A DEP-based single cell trapping (Qin et al 2018 ), B optical tweezer-based single cell trapping (Cong et al 2019 ), C magnetic-based single cell trapping (Shields et al 2016 ) …”
Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning
confidence: 99%
“…This platform can achieve selective capture of single cells with light beams (Huang et al 2013 ). For increasing the range of optical trapping, Cong et al ( 2019 ) made a focused optical beam trap by combining optical forces and convective drag forces to guide living single cancer cell into a microwell array for multidrug-resistant gene marker detection (Fig. 4 B).…”
Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning
Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.
“…Considering the undulatory property of light 30 , the finite difference time domain(FDTD) method and "COMSOL" are operated to simulate the thermal energy of highly focused beam 31,32 and fluidity of liquid samples respectively 33 . The reason is that under the laser irradiation, the radiation pressure may heat the liquid solution 34,35 , affecting the laminar flow in the chamber 36 . Due to the loss of laser in the optical path, the final power of single optical trap is less than 600 mW in the sample chamber.…”
Utilizing droplets as micro-tools has become a valuable method in biology and chemistry. In previous work, we have demonstrated a novel droplets generation-manipulation method in a conventional optical tweezers system....
“…Optothermal microfluidic techniques have had a significant impact on physiological and clinical use in recent years. The optothermal microfluidic has enabled living cell trapping and manipulations, where plasmonic heat‐induced natural convection and thermophoresis provides a larger manipulation range . Because of its unique properties of remote control and ultrafast on‐demand nanoscale thermal production, it has also been widely employed in biosensing and manipulation of cellular functions, such as ultrafast plasmonic PCR, intracellular gene delivery and release, as well as single‐cell neural activity control …”
Thermal optofluidics is an emerging field that promises to create numerous research and application opportunities in biophysics, biochemistry, and clinical biology. Innovation in plasmonic optics has led to the development of various invaluable tools in the fields of biosensing and microfluidic manipulation. The optothermal effect originates from light–matter interactions during photon–phonon conversion, which can lead to micro‐ or nanoscale inhomogeneities in the thermal distribution. This further induces a series of hydrodynamic phenomena such as natural convection, Marangoni convection, thermophoresis, the electrolyte Seebeck effect, depletion forces, and interfacial effects in colloidal particles. Light–matter interactions are particularly important for three aspects of microfluidics, namely the motion of colloidal particles, fluidic actuation, and biochemical reactions. This review first systematically elucidates the role of both nanoscale plasmonic thermal generation and heat‐induced fluidic motion in optofluidic microsystems. Then, recent state‐of‐the‐art thermal optofluidic applications of the above‐listed three aspects are presented. The paper aims to provide an insightful reference for future research in optofluidic biochemical systems.
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