Target-catalyzed hairpin structure-mediated padlock cyclization for ultrasensitive rolling circle amplification

Song, Huanxia; Yang, Zhe; Jiang, Min; Zhang, Guo; Gao, Yansha; Wu, Zai‐Sheng; Lou, Yongliang

doi:10.1016/j.talanta.2019.05.057

Cited by 12 publications

(4 citation statements)

References 60 publications

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“…This study was conducted using target-catalyzed hairpin structure-mediated padlock cyclization for the ultrasensitive detection of human proto-oncogenes. Here, the author described THP-RCA that could be performed in human serum conditions along with a detection limit of 100 fM . Nucleic acid fluorescent probe engineering has been vastly studied in recent times, and the involvement of fluorophores and chemically modified linkage in molecular diagnostics have been unraveled significantly since the fluorophore utilization/conjugation interest the use of functional nanomaterials to explore their intrinsic properties for DNA/nucleic acid sensing. , …”

Section: Dna Probe-designed Nucleic Acid Detectionmentioning

confidence: 99%

DNA and Nanomaterials: A Functional Combination for DNA Sensing

Quazi,

Choi,

Kim

et al. 2024

ACS Appl. Bio Mater.

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Recent decades have experienced tough situations due to the lack of reliable diagnostic facilities. The most recent cases occurred during the pandemic, where researchers observed the lack of diagnostic facilities with precision. Microorganisms and viral disease's ability to escape diagnosis has been a global challenge. DNA always has been a unique moiety with a strong and precise base-paired structure. DNA in human and foreign particles makes identification possible through base pairing. Since then, researchers have focused heavily on designing diagnostic assays targeting DNA in particular. Moreover, DNA nanotechnology has contributed vastly to designing composite nanomaterials by combining DNA/nucleic acids with functional nanomaterials and inorganic nanoparticles exploiting their physicochemical properties. These nanomaterials often exhibit unique or enhanced properties due to the synergistic activity of the many components. The capabilities of DNA and additional nanomaterials have shown the combination of robust and advanced tailoring of biosensors. Preceding findings state that the conventional strategies have exhibited certain limitations such as a low range of target detection, less biodegradability, subordinate half-life, and high susceptibility to microenvironments; however, a DNA−nanomaterial-based biosensor has overcome these limitations meaningfully. Additionally, the unique properties of nucleic acids have been studied extensively due to their high signal conduction abilities. Here, we review recent studies on DNA−nanomaterial-based biosensors, their mechanism of action, and improved/updated strategies in vivo and in situ. Furthermore, this review highlights the recent methodologies on DNA utilization to exploit the interfacial properties of nanomaterials in DNA sensing. Lastly, the review concludes with the limitations/challenges and future directions.

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Section: Dna Probe-designed Nucleic Acid Detectionmentioning

confidence: 99%

DNA and Nanomaterials: A Functional Combination for DNA Sensing

Quazi,

Choi,

Kim

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…In the integration of RCA with CHA, two common ways were chosen, including CHA product as RCA primer (CHA-RCA) [ [105] , [106] , [107] , [108] ] and RCA product as CHA trigger (RCA-CHA) [ 109 , 110 ]. In addition, a nicking enzyme was used in RCA-CHA to deal with the problem of hindering the initiation of CHA by RCA products [ 111 ].…”

Section: Semi-enzyme-free Cascade Amplificationmentioning

confidence: 99%

Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection

Jiang

et al. 2023

Talanta

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“…Then, HP2 is cyclized by ligase on the HP1 template; the cyclized HP2 enables the RCA and generates a long tandem ssDNA product that is capable of hybridizing with considerable quantity of MBs. Subsequently, the amplified fluorescence value represents the ultrasensitive detection of STAT3 gene ( Song et al, 2019 ). Moreover, the sensing system is suitable for target detection in human serum.…”

Section: The Application Of Nucleic Acid Probe–based Fluorescent Sensing In Cancer Diagnosis and Treatmentmentioning

confidence: 99%

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

Huang

Wang

et al. 2021

Front. Chem.

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It is well known that cancer incidence and death rates have been growing, but the development of cancer theranostics and therapeutics has been a challenging work. Recently, nucleic acid probe–based fluorescent sensing and imaging have achieved remarkable improvements in a variety of cancer management techniques, credited to their high sensitivity, good tolerance to interference, fast detection, and high versatility. Herein, nucleic acid probe–based fluorescent sensing and imaging are labeled with advanced fluorophores, which are essential for fast and sensitive detection of aberrant nucleic acids and other cancer-relevant molecules, consequently performing cancer early diagnosis and targeted treatment. In this review, we introduce the characteristics of nucleic acid probes, summarize the development of nucleic acid probe–based fluorescent sensing and imaging, and prominently elaborate their applications in cancer diagnosis and treatment. In discussion, some challenges and perspectives are elaborated in the field of nucleic acid probe–based fluorescent sensing and imaging.

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Target-catalyzed hairpin structure-mediated padlock cyclization for ultrasensitive rolling circle amplification

Cited by 12 publications

References 60 publications

DNA and Nanomaterials: A Functional Combination for DNA Sensing

DNA and Nanomaterials: A Functional Combination for DNA Sensing

Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

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