TaPHT1;9‐4B and its transcriptional regulator TaMYB4‐7D contribute to phosphate uptake and plant growth in bread wheat

Wang, Pengfei; Li, Gezi; Li, Guangwei; Yuan, Shasha; Wang, Chenyang; Xie, Yingxin; Guo, Tao; Kang, Guozhang; Wang, Daowen

doi:10.1111/nph.17534

Cited by 22 publications

(20 citation statements)

References 64 publications

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“…Additionally, the root Pi concentration was also increased in PdeWRKY6 OE lines under low Pi availability, and this also supports our hypothesis. These observations are consistent with the functions of PHT1;9 in other plants (Remy et al, 2012;Wang et al, 2021).…”

Section: Discussionsupporting

confidence: 91%

Phosphate (Pi) stress‐responsive transcription factors PdeWRKY6 and PdeWRKY65 regulate the expression of PdePHT1;9 to modulate tissue Pi concentration in poplar

et al. 2022

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Phosphorus (P) is an important nutrient for plants. Here, we identify a WRKY transcription factor (TF) in poplar (Populus deltoides 3 Populus euramericana) (PdeWRKY65) that modulates tissue phosphate (Pi) concentrations in poplar. PdeWRKY65 overexpression (OE) transgenic lines showed reduced shoot Pi concentrations under both low and normal Pi availabilities, while PdeWRKY65 reduced expression (RE) lines showed the opposite phenotype. A gene encoding a Pi transporter (PHT), PdePHT1;9, was identified as the direct downstream target of PdeWRKY65 by RNA sequencing (RNA-Seq). The negative regulation of PdePHT1;9 expression by PdeWRKY65 was confirmed by DNA-protein interaction assays, including yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), co-expression of the promoters of PdePHT1;9 and PdeWRKY65 in tobacco (Nicotiana benthamiana) leaves, and chromatin immunoprecipitation-quantitative PCR. A second WRKY TF, PdeWRKY6, was subsequently identified and confirmed to positively regulate the expression of PdePHT1;9 by DNA-protein interaction assays. PdePHT1;9 and PdeWRKY6 OE and RE poplar transgenic lines were used to confirm their positive regulation of shoot Pi concentrations, under both normal and low Pi availabilities. No interaction between PdeWRKY6 and PdeWRKY65 was observed at the DNA or protein levels. Collectively, these data suggest that the low Pi-responsive TFs PdeWRKY6 and PdeWRKY65 independently regulate the expression of PHT1;9 to modulate tissue Pi concentrations in poplar.

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Section: Discussionsupporting

confidence: 91%

Phosphate (Pi) stress‐responsive transcription factors PdeWRKY6 and PdeWRKY65 regulate the expression of PdePHT1;9 to modulate tissue Pi concentration in poplar

et al. 2022

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“…However, there are two fragments (457 bp and 246 bp) in other haplotypes after their promoter are digested by Fnu 4HI. Therefore, Hap3 wheat cultivars of TaPHT1;9-4B are easily distinguished (Wang et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

Wang,

Chen,

Shi

et al. 2024

Preprint

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Context: In our previous study, TaPHT1;9-4B, one key high-affinity Pi transporter, was found to greatly contribute to Pi acquisition and transportation, and its functional marker CAPS-799 was subsequently developed to identify its Pi-efficient elite haplotype. Objective: The study aimed to screen a varieties of wheat cultivars by using the above CAPS-799, identify its Pi-efficient elite haplotype cultivars, and reveal its physiological mechanism. Methods: Successive two-year field experiments without Pi fertilizer supply, and hydroponic experiment with low Pi (10 μM) were performed. P concentrations, biomasses, grain yields, yield components, root growth parameters, and TaPHT1;9-4B transcript levels were measured. Total P accumulation and transport efficiency, and the relative growth rates were calculated. Results: Eight Pi-efficient wheat cultivars (Hap3 haplotype) were screened out by using the CAPS-799 from 80 modern major cultivars, and in successive two-year field experiments, their grain yields, spike numbers, P absorption and transport efficiencies were significantly higher than those of Non-Hap3 haplotypes (Hap1, 2 and 4) under no Pi fertilizer supply condition, and therefore, these eight cultivars belonged to Pi-efficient elite haplotype. TaPHT1;9-4B transcript levels in roots at the early stage of grain filling period in field experiment, and the relative growth rates of total root surface areas, volumes and mean root diameters of Hap3 cultivars in hydroponic experiment, were markedly higher than other haplotypes. Conclusions: CAPS-799 was a useful functional marker for screening Pi-efficient wheat cultivars, and its Pi-efficient wheat cultivars were characterized with higher TaPHT1;9-4B transcript levels and more roots. Implications: CAPS-799 will be used to screen or develop Pi-efficient wheat cultivars.

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“…Phenotypic differences in winter frost tolerance and agronomic traits among cultivars were tested using analysis of variance (ANOVA) in the SPSS software 26.0 (International Business Machines Corporation, Armonk, NY, United States), and multiple comparison analysis was performed using the least significant difference (LSD) test at P < 0.05. The differences in winter frost between genotypes in the association population were determined by the t -test in the SPSS software 26.0 ( Wang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Association of the Recessive Allele vrn-D1 With Winter Frost Tolerance in Bread Wheat

et al. 2022

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Winter frost has been considered the primary limiting factor in wheat production. Shimai 12 is an elite wheat cultivar grown in central and southern Hebei province of China, but sensitive to winter frost. In this study, the winter frost tolerant cultivar Lunxuan 103 was bred by introducing the recessive allele vrn-D1 from winter wheat Shijiazhuang 8 (frost tolerance) into Shimai 12 using marker-assisted selection (MAS). Different from Shimai 12, Lunxuan 103 exhibited a winter growth habit with strong winter frost tolerance. In the Shimai 12 × Shijiazhuang 8 population, the winter progenies (vrn-D1vrn-D1) had significantly lower winter-killed seedling/tiller rates than spring progenies (Vrn-D1aVrn-D1a), and the consistent result was observed in an association population. Winter frost damage caused a significant decrease in grain yield and spike number/m2 in Shimai 12, but not in Lunxuan 103 and Shijiazhuang 8. The time-course expression analysis showed that the transcript accumulation levels of the cold-responsive genes were higher in Lunxuan 103 and Shijiazhuang 8 than in Shimai 12. Lunxuan 103 possessed the same alleles as its parents in the loci for plant height, vernalization, and photoperiod, except for the vernalization gene Vrn-D1. An analysis of genomic composition showed that the two parents contributed similar proportions of genetic compositions to Lunxuan 103. This study provides an example of the improvement of winter frost tolerance by introducing the recessive vernalization gene in bread wheat.

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TaPHT1;9‐4B and its transcriptional regulator TaMYB4‐7D contribute to phosphate uptake and plant growth in bread wheat

Cited by 22 publications

References 64 publications

Phosphate (Pi) stress‐responsive transcription factors PdeWRKY6 and PdeWRKY65 regulate the expression of PdePHT1;9 to modulate tissue Pi concentration in poplar

Phosphate (Pi) stress‐responsive transcription factors PdeWRKY6 and PdeWRKY65 regulate the expression of PdePHT1;9 to modulate tissue Pi concentration in poplar

Functional marker CAPS-799 of theTaPHT1;9-4Bgene is useful for screening phosphorus-efficient wheat cultivars

Association of the Recessive Allele vrn-D1 With Winter Frost Tolerance in Bread Wheat

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