2022
DOI: 10.3389/fpls.2022.879768
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Association of the Recessive Allele vrn-D1 With Winter Frost Tolerance in Bread Wheat

Abstract: Winter frost has been considered the primary limiting factor in wheat production. Shimai 12 is an elite wheat cultivar grown in central and southern Hebei province of China, but sensitive to winter frost. In this study, the winter frost tolerant cultivar Lunxuan 103 was bred by introducing the recessive allele vrn-D1 from winter wheat Shijiazhuang 8 (frost tolerance) into Shimai 12 using marker-assisted selection (MAS). Different from Shimai 12, Lunxuan 103 exhibited a winter growth habit with strong winter fr… Show more

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“…The primer information of all the markers is listed in Supplementary Table S10 . The primers specific for genes conferring plant height and vernalization were used to differentiate the alleles in recurrent parents, donors and promising DH lines according to the methods described by Zhang et al [ 35 ]. Polymerase chain reaction (PCR) was performed with an initial denaturation at 94 °C for 5 min, 35 cycles of 94 °C for 30 s and annealing at 60–64 °C for 30 s and 72 °C for 30 s–2 min, with a final extension at 72 °C for 10 min.…”
Section: Methodsmentioning
confidence: 99%
“…The primer information of all the markers is listed in Supplementary Table S10 . The primers specific for genes conferring plant height and vernalization were used to differentiate the alleles in recurrent parents, donors and promising DH lines according to the methods described by Zhang et al [ 35 ]. Polymerase chain reaction (PCR) was performed with an initial denaturation at 94 °C for 5 min, 35 cycles of 94 °C for 30 s and annealing at 60–64 °C for 30 s and 72 °C for 30 s–2 min, with a final extension at 72 °C for 10 min.…”
Section: Methodsmentioning
confidence: 99%