Tannins from barks of Pinus caribaea protect Escherichia coli cells against DNA damage induced by γ-rays

Fuentes, Jorge Luı́s; Vernhe, Mariolys; Cuétara, Elizabeth; Sánchez-Lamar, Ángel; Santana, Jorge Luis; Llagostera, Montserrat

doi:10.1016/j.fitote.2005.11.014

Cited by 20 publications

(10 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data suggest an antigenotoxic action of both extracts based on free radical scavenging mechanisms. Related to this, we have recently demonstrated (Fuentes et al 2006), that the tannins fraction of P. caribaea is the main constituent responsible for antigenotoxicity detected here against g-rays. On the other hand, an antioxidant activity of C. citratus extract has also been shown in CHO cells treated with hydrogen peroxide (Cápiro et al 2001).…”

Section: Discussionsupporting

confidence: 74%

Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors

Fuentes

Alonso

Cuétara³

et al. 2006

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 74%

Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors

Fuentes

Alonso

Cuétara³

et al. 2006

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

“…Antigenotoxicity was assayed using the co-incubation procedure, as indicated by Fuentes et al , (2006). Although the procedure was basically the same as that of the genotoxicity protocol, the cells were simultaneously co-treated with different concentrations of the tested substances (EO, citral, carvone and limonene) and the mutagen (1 μg/mL of bleomycin).…”

Section: Methodsmentioning

confidence: 99%

Chemical composition and antigenotoxic properties of Lippia alba essential oils

2011

View full text Add to dashboard Cite

The present work evaluated the chemical composition and the DNA protective effect of the essential oils (EOs) from Lippia alba against bleomycin-induced genotoxicity. EO constituents were determined by Gas Chromatography/Mass Spectrometric (GC-MS) analysis. The major compounds encountered being citral (33% geranial and 25% neral), geraniol (7%) and trans-β-caryophyllene (7%) for L. alba specimen COL512077, and carvone (38%), limonene (33%) and bicyclosesquiphellandrene (8%) for the other, COL512078. The genotoxicity and antigenotoxicity of EO and the compounds citral, carvone and limonene, were assayed using the SOS Chromotest in Escherichia coli. The EOs were not genotoxic in the SOS chromotest, but one of the major compound (limonene) showed genotoxicity at doses between 97 and 1549 mM. Both EOs protected bacterial cells against bleomycin-induced genotoxicity. Antigenotoxicity in the two L. alba chemotypes was related to the major compounds, citral and carvone, respectively. The results were discussed in relation to the chemopreventive potential of L. alba EOs and its major compounds.

show abstract

“…The cells were exposed to serum samples for 30 min at 8 °C and then cultured for 2 h at 37 °C. β-galactosidase and alkaline phosphatase activities were assayed as described by Fuentes et al , (2006). The criterion for genotoxicity was the SOS induction factor (SOSIF), as defined by Quillardet et al (1989): SOSIF = [β-galactosidase/alkaline phosphatase] treatment /[β-galactosidase/alkaline phosphatase] negative control .…”

Section: Methodsmentioning

confidence: 99%

Estimates of DNA strand breakage in bottlenose dolphin (Tursiops truncatus) leukocytes measured with the Comet and DNA diffusion assays

et al. 2009

View full text Add to dashboard Cite

The analysis of DNA damage by mean of Comet or single cell gel electrophoresis (SCGE) assay has been commonly used to assess genotoxic impact in aquatic animals being able to detect exposure to low concentrations of contaminants in a wide range of species. The aims of this work were 1) to evaluate the usefulness of the Comet to detect DNA strand breakage in dolphin leukocytes, 2) to use the DNA diffusion assay to determine the amount of DNA strand breakage associated with apoptosis or necrosis, and 3) to determine the proportion of DNA strand breakage that was unrelated to apoptosis and necrosis. Significant intra-individual variation was observed in all of the estimates of DNA damage. DNA strand breakage was overestimated because a considerable amount (~29%) of the DNA damage was derived from apoptosis and necrosis. The remaining DNA damage in dolphin leukocytes was caused by factors unrelated to apoptosis and necrosis. These results indicate that the DNA diffusion assay is a complementary tool that can be used together with the Comet assay to assess DNA damage in bottlenose dolphins.

show abstract

Tannins from barks of Pinus caribaea protect Escherichia coli cells against DNA damage induced by γ-rays

Cited by 20 publications

References 8 publications

Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors

Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors

Chemical composition and antigenotoxic properties of Lippia alba essential oils

Estimates of DNA strand breakage in bottlenose dolphin (Tursiops truncatus) leukocytes measured with the Comet and DNA diffusion assays

Contact Info

Product

Resources

About