Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors

Fuentes, Jorge Luı́s; Alonso, Alena; Cuétara, Elizabeth; Vernhe, Mariolys; Álvarez, Nadine; Sánchez-Lamar, Ángel; Llagostera, Montserrat

doi:10.1080/09553000600733168

Cited by 15 publications

(7 citation statements)

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“…We have previously proposed the SOS chromotest as a potentially useful assay for primary screening of medicinal plant extracts for antigenotoxic activity against γ-rays (Fuentes et al , 2006a). With this test, we have observed that the use of DMSO as solvent resulted in overestimates of the antimutagenic activity of essential oils when compared with distilled water (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…This assay, which is based on a colorimetric assessment of SOS transcriptional gene fusion in Escherichia coli PQ37, allows one to estimate primary DNA damage produced by chemical and physical mutagens by measuring the expression of a reporter gene, β-galactosidase (Quillardet and Hofnung, 1993). Compared to other methods, the SOS chromotest allows greater throughput when screening natural compounds (Fuentes et al , 2006a). …”

Section: Introductionmentioning

confidence: 99%

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The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest

2012

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In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC) and 4-nitroquinoline-1-oxide (4-NQO) were also investigated. None of the solvents were genotoxic in Escherichia coli PQ37. However, based on the inhibition of protein synthesis assessed by constitutive alkaline phosphatase activity, some solvents (carbon tetrachloride, dimethylsulfoxide, ethanol and ether) were toxic and incompatible with the SOS chromotest. Solvents that were neither toxic nor genotoxic to E. coli (acetone, dichloromethane and methanol) significantly reduced the genotoxicity of MMC and 4-NQO. When these solvents were used to dissolve vitamin E they increased the antigenotoxic activity of this compound, possibly through additive or synergistic effects. The relevance of these results is discussed in relation to antigenotoxic studies. These data indicate the need for careful selection of an appropriate diluent for the SOS chromotest since some solvents can modulate genotoxicity and antigenotoxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest

2012

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“…However, the presence of POE significantly protected DNA molecules from CPD formation and UVB-ROS generation. For this extract free oxygen radical scavenging activity, was previously demonstrated, which contributes to UVB-photooxidative protective effect [5,[17][18][19][20]. According to the UVB transmittance of CF and DF, none of these fractions protected DNA against the damages induced by UVB light.…”

Section: Discussionmentioning

confidence: 99%

Photoprotection of Fractions Obtained from Phyllanthus Orbicularis Extract against UVB -Induced DNA Damage

2018

DERM

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The use of plants as sources of photochemopreventive agents has been increased in the last years. The assessment of photoprotective properties of plant extracts improves the development of pharmaceutical and cosmetic products. In the present work, we evaluate the effect of Phyllanthus orbicularis extracts against UVB-induced damage to DNA. The total aqueous extract of this plant (POE) and chemical fractions obtained from it: aqueous (AF), chloroformic (CF) and dichloromethane (DF) were assessed. For this, plasmid DNA solutions were exposed to UVB radiation in presence of plant extracts. The DNA repair enzymes Formamidopyrimidine-DNA glycosylase (Fpg) and T4 bacteriophage endonuclease V (T4-endo V) were employed to distinguish oxidized DNA and cyclobutane pyrimidine dimer (CPD) lesions. The supercoiled and relaxed forms of DNA were separated through electrophoretic migration in agarose gels. Additionally, the UVB wavelength transmittances of total extract and chemical fractions of P.orbicularis were measured. The results showed that POE and AF protect DNA against the damage induced by UVB light. This protective effect is related to the antioxidants and absorptive properties of some flavonoids present in the extracts.

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“…Similar modifications were also introduced to the SOS chromotest (Ohta et al, 1984). The original colorimetric procedure of the assay (Quillardet et al, 1982) was successfully changed to a fluorimetric one by using the fluorescent substrate 4-methylumbelliferyl-b-Dgalactopyranoside (MUG) (Fuentes et al, 2006).…”

Section: Reporter Systemsmentioning

confidence: 99%

Bacterial genotoxicity bioreporters

Biran

Yagur‐Kroll

Pedahzur

et al. 2010

Microbial Biotechnology

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SummaryEver since the introduction of the Salmonella typhimurium mammalian microsome mutagenicity assay (the ‘Ames test’) over three decades ago, there has been a constant development of additional genotoxicity assays based upon the use of genetically engineered microorganisms. Such assays rely either on reversion principles similar to those of the Ames test, or on promoter–reporter fusions that generate a quantifiable dose‐dependent signal in the presence of potential DNA damaging compounds and the induction of repair mechanisms; the latter group is the subject of the present review. Some of these assays were only briefly described in the scientific literature, whereas others have been developed all the way to commercial products. Out of these, only one, the umu‐test, has been fully validated and ISO‐ and OECD standardized. Here we review the main directions undertaken in the construction and testing of bacterial‐based genotoxicity bioassays, including the attempts to incorporate at least a partial metabolic activation capacity into the molecular design. We list the genetic modifications introduced into the tester strains, compare the performance of the different assays, and briefly describe the first attempts to incorporate such bacterial reporters into actual genotoxicity testing devices.

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Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors

Abstract: The results have demonstrated the usefulness of the modified SOS Chromotest assay in the screening of phytochemical radioprotectors as well as in the study of their antimutagenic mechanisms.

Cited by 15 publications

References 50 publications

The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest

The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest

Photoprotection of Fractions Obtained from Phyllanthus Orbicularis Extract against UVB -Induced DNA Damage

Bacterial genotoxicity bioreporters

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