Tannin-tolerant bacteria from crossbred Holstein x Zebu cows

Abstract

-The objective of this work was to isolate and characterize tannin-tolerant ruminal bacteria from crossbred Holstein x Zebu cows fed a chopped mixture of elephant grass (Pennisetum purpureum), young stems of "angico-vermelho" (Parapiptadenia rigida), and banana tree (Musa sp.) leaves. A total of 117 bacteria strains were isolated from enrichment cultures of rumen microflora in medium containing tannin extracts. Of these, 11 isolates were able to tolerate up to 3 g L -1 of tannins. Classical characterization procedures indicated that different morphological and physiological groups were represented. Restriction fragments profiles using Alu1 and Taq1 of 1,450 bp PCR products from the 16S rRNA gene grouped the 11 isolates into types I to VI. Sequencing of 16S rRNA PCR products was used for identification. From the 11 strains studied, seven were not identifiable by the methods used in this work, two were strains of Butyrivibrio fibrisolvens, and two of Streptococcus bovis.

Nutritive value of treatedQuercus infectoriaandQuercus libanileaves with the tannin‐degrading bacteriumKlebsiella pneumoniaefor ruminant feedingin vitro

“…Therefore, this study was planned to investigate the chemical composition, in vitro gas production (IVGP) and fermentation parameters of Q. infectoria and Q. libani oak leaf following treatment with Klebsiella pneumoniae , a native tannase producing bacterium. The K. pneumoniae used in the present study was isolated from farm soil and this is consistent with other studies that isolated several bacterial strains with phenolic degrading potential from different sources (Arcuri et al ; Pepi et al ; Mosleh et al ).…”

Modulatory effects of dietary tannins on polyunsaturated fatty acid biohydrogenation in the rumen: A meta-analysis

“…This indicates that there are variations in some rumen fermentation profile variables in certain tannin level categories between the in vitro and in vivo rumen studies. The study by Arcuri et al [53] found that the B. fibrisolvens and Streptococcus bovis strains isolated from Holstein  Zebu cattle resisted the biological effect of CT Mimosa artemisia extract at low levels (2-3 g L À1 ). The effect of eliminating the rumen methanogen population using tannin-sourced feeds has a direct impact on reducing the overall level of ruminant methane production.…”