Tannin acyl-hydrolase production by Bacillus subtilis KMS2-2: Purification, characterization, and cytotoxicity studies

Govindarajan, Rasiravathanahalli Kaveriyappan; Mathivanan, Krishnamurthy; Khanongnuch, Chartchai; Srinivasan, R.; Unban, Kridsada; Arulanandam, Charli Deepak; Farraj, Dunia A. Al; Alarjani, Khaloud Mohammed; Qahtany, Fatmah S. Al

doi:10.1016/j.jksus.2021.101359

Cited by 16 publications

(12 citation statements)

References 32 publications

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“…Cations act as salt and complex ions to preserve tannase conformation or to stabilize the binding of a specific substratum molecule as a cofactor [ 1 ] rather than to promote the catalytic reaction of the enzyme. It is believed that the rigid conformation of the enzyme molecule can be maintained by a salt or ion bridge via a cluster of carboxylic groups of the enzyme.…”

Section: Discussionmentioning

confidence: 99%

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Acid Stable Yeast Cell-Associated Tannase with High Capability in Gallated Catechin Biotransformation

et al. 2021

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Previously, nine tannin-tolerant and tannase-producing yeasts were isolated from Miang; all produced cell-associated tannase (CAT) during growth in tannin substrate. Among which, only CAT from Sporidiobolus ruineniae showed better stability than its purified form. Yet, it is of particular interest to directly characterize CATs from the latter yeasts. In this study, four CATs from yeasts, namely Cyberlindnera rhodanensis A22.3, Candida sp. A39.3, Debaryomyces hansenii A45.1, and Cy. rhodanensis A45.3 were characterized. The results indicate that all CATs were produced within the same production yield (11 mU/mL). Most CATs exhibited similar pH and temperature optima and stabilities, except for CAT from Cy. rhodanensis A22.3. This CAT was assigned as acid-stable tannase due to its unusual optimum pH of 2.0 with pH stability and half-life thermostability in the range of pH 2.0–4.0, and 70 °C, respectively. All CATs demonstrated high substrate specificity toward epigallocatechin gallate and epicatechin gallate, thus forming epigallocatechin and epicatechin, respectively. Moreover, they showed operational stability to repeated use for up to five cycles without loss of the initial activity. Therefore, CATs from these yeasts could be useful for the extraction and biotransformation of tea catechins and related applications.

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Section: Discussionmentioning

confidence: 99%

“…Tannins are naturally found in plant materials as water-soluble polyphenols with varying molecular weight [ 1 ]. They have been classified into three categories including hydrolysable tannins, condensed tannins, and complex tannins.…”

Section: Introductionmentioning

confidence: 99%

Acid Stable Yeast Cell-Associated Tannase with High Capability in Gallated Catechin Biotransformation

et al. 2021

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“…Synthetic and semi-synthetic cannabinoids are very important for the economy owing to demands from the wholesale market and various cannabis industries to enhance agri-business and prevent plant pathogens [ 108 ]. The hypothesis is that phytocannabinoids have versatile use and are beneficial for humans and plants if appropriately used [ 109 , 110 ]. Further investigations are needed on the genes and enzymes involved in the biosynthetic pathways in different plant species [ 111 , 112 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Biosynthesis of Phytocannabinoids and Structural Insights: A Review

et al. 2023

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Cannabis belongs to the family Cannabaceae, and phytocannabinoids are produced by the Cannabis sativa L. plant. A long-standing debate regarding the plant is whether it contains one or more species. Phytocannabinoids are bioactive natural products found in flowers, seeds, and fruits. They can be beneficial for treating human diseases (such as multiple sclerosis, neurodegenerative diseases, epilepsy, and pain), the cellular metabolic process, and regulating biological function systems. In addition, several phytocannabinoids are used in various therapeutic and pharmaceutical applications. This study provides an overview of the different sources of phytocannabinoids; further, the biosynthesis of bioactive compounds involving various pathways is elucidated. The structural classification of phytocannabinoids is based on their decorated resorcinol core and the bioactivities of naturally occurring cannabinoids. Furthermore, phytocannabinoids have been studied in terms of their role in animal models and antimicrobial activity against bacteria and fungi; further, they show potential for therapeutic applications and are used in treating various human diseases. Overall, this review can help deepen the current understanding of the role of biotechnological approaches and the importance of phytocannabinoids in different industrial applications.

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“…The main importance of tannase was the production of gallic acid. It was also used in various commercial applications in beverages industrial product, food, pharmaceutical, chemical [5,6], feed [1], wine, beer, coffee [7]. Gallic acid, commonly known as trihydroxy benzoic acid, is an organic acid molecules found in various parts of plants [8], most commonly in tea leaves [9].…”

Section: Introductionmentioning

confidence: 99%

Screening and optimization of a novel gallic acid and tannase production under semi quantitative and quantitative methods

Abdulshaheed,

Hanafiah,

Muslim

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Tannin acyl hydrolase as the common name of tannase is an inducible extracellular enzyme that causes the hydrolysis of galloyl ester and depside bonds in tannins, yielding gallic acid and glucose. The main objective of this study is to find a novel gallic acid and tannase produced by Acinetobacter Baumannii. A number of optimization steps were followed in order to improve the highest production of gallic acid and tannase. In present study, A. baumannii were isolated from ICU burn. A. baumannii was examined by microscopic examination, morphological and biochemical assay including oxidase and catalase. The following parameters were considered and determined the effect of pH, temperature, incubation period and inoculum volume on gallic acid and tannase production. It was observed that A. baumannii gave the highest yields of gallic acid and tannase using semi-quantitative and quantitative methods. A novel A. baumannii stimulated production of gallic acid and tannase using 0.5% tannic acid in agar medium. The maximum production of gallic acid and tannase by A. baumannii was recorded at pH 5.5, temperature at 37°C, 72h of incubation period and 10% of the inoculum volume. The results concluded that the highest yields of gallic acid (47.54 mg/ml) and tannase (50.12U/mg) were obtained under improved conditions.

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Tannin acyl-hydrolase production by Bacillus subtilis KMS2-2: Purification, characterization, and cytotoxicity studies

Cited by 16 publications

References 32 publications

Acid Stable Yeast Cell-Associated Tannase with High Capability in Gallated Catechin Biotransformation

Acid Stable Yeast Cell-Associated Tannase with High Capability in Gallated Catechin Biotransformation

Biosynthesis of Phytocannabinoids and Structural Insights: A Review

Screening and optimization of a novel gallic acid and tannase production under semi quantitative and quantitative methods

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