Tannic acid modulates NFκB signaling pathway and skin inflammation in NC/Nga mice through PPARγ expression

Karuppagounder, Vengadeshprabhu; Arumugam, Somasundaram; Thandavarayan, Rajarajan Amirthalingam; Pitchaimani, Vigneshwaran; Sreedhar, Remya; Afrin, Rejina; Harima, Meilei; Sano, Hiroshi; Nomoto, Mayumi; Miyashita, Shizuka; Suzuki, Kenji; Nakamura, Masahiko; Ueno, K.; Watanabe, Kenichi

doi:10.1016/j.cyto.2015.05.016

Cited by 54 publications

(38 citation statements)

References 42 publications

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“…As reported, tannins exerted anti-proliferative activities to many cancer cell lines such as MCF-7, Caco-2 and DU145, 30 and it could effectively treat kinds of human diseases including inammation, Type II diabetes mellitus and leukemia. [31][32][33] Hence, the KEGG results basically conform to the reported facts. In the ZG group, all the DEGs were enriched in metabolic pathways, namely, ABC transporters, purine metabolism and drug metabolism-other enzymes.…”

Section: The Transcriptomics Study Of Dy and Its Main Components On Tsupporting

confidence: 76%

Aqueous extract of Sanguisorba officinalis blocks the Wnt/β-catenin signaling pathway in colorectal cancer cells

Liu

Dai

et al. 2018

RSC Adv.

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Sanguisorba officinalis (the Chinese name is DiYu, DY) exerts significant anti-proliferative activities against colorectal cancer (CRC) cells. Since most of CRC result from the aberrant activation of the Wnt/b-catenin signaling pathway, inhibitors of the Wnt pathway are considered as promising anti-CRC agents. This study aimed to investigate whether DY could be a potential herbal Wnt inhibitor, and the bioactive constituents and underlying molecular mechanisms for DY's inhibiting activities would be studied as well. Accordingly, the inhibitory activities of DY and its main components against the Wnt pathway were assessed using the single-luciferase reporter assay based on HEK293 cells. Additionally, the levels of key Wnt-related genes or proteins were measured to verify the inhibitory effects on the Wnt pathway of CRC cells. Finally, the underlying mechanisms accounting for the efficacy of candidate drugs were explored by the transcriptomic study. Results show that DY and its tannins (RZ), and saponins (ZG) significantly inhibited the Wnt pathway of HEK293 cells activated by wnt3a. However, their respective constituents were not effective as expected. Additionally, DY and RZ prominently down-regulated the levels of b-catenin and Wnt-targeted genes including Axin2, c-Myc or CyclinD1 of three CRC cells. Transcriptomic profiling study suggests that the down-regulation of the mRNA levels of Wnt-related genes such as LPAR6 may be associated with the inhibitory effects of DY and RZ on the Wnt pathway of HT29 cells. Therefore, our studies first uncovered the blocking activity of DY on the Wnt pathway, providing evidence for the rationale of developing Wnt inhibitors from DY as anti-CRC agents.

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Section: The Transcriptomics Study Of Dy and Its Main Components On Tsupporting

confidence: 76%

Aqueous extract of Sanguisorba officinalis blocks the Wnt/β-catenin signaling pathway in colorectal cancer cells

Liu

Dai

et al. 2018

RSC Adv.

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“…Plant derived antioxidant compounds are considered safe to be used as possible agents for the inhibition of inflammation (Mut‐Salud et al, ). In that case, tannic acid is a natural polyphenol which are applied to improve human health, including treatments of ulcers, burns, hemorrhoids, stomatitis, tonsillitis, (Nie et al, ), antioxidant, anti‐inflammatory, anticarcinogenic, antimutagenic, antitumor, and antimicrobial activities (Karuppagounder et al, ; Sen et al, ). Tannic acid can be easily found in leaves, roots, bark, and fruits of diverse plant varieties, especially in tea, grapes, coffee, and other plants (Gulįin, Huyut, Elmastas, & Aboul‐Enein, ).…”

Section: Introductionmentioning

confidence: 99%

Anti‐neuroinflammatory effects of tannic acid against lipopolysaccharide‐induced BV2 microglial cells via inhibition of NF‐κB activation

Zhong

et al. 2019

Drug Development Research

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Microglia mediated neuroinflammation is known to cause various neurodegenerative and neurological ailments. Tannic acid is a natural polyphenol which has been reported to possess antioxidant, anti‐inflammatory, anticarcinogenic, antimutagenic, antitumor, and antimicrobial activities. As there are no reports till date on the anti‐neuroinflammatory effects of tannic acid, this study was conducted to analyze the possible mechanism and pathway involved in the prevention of neuroinflammation by tannic acid in BV2 microglial cells. BV2 microglial cells were pretreated with tannic acid (10, 25, and 50 μM/mL) and induced with lipopolysaccharide (LPS; 1 μM/mL) to assess the production of reactive oxygen species (ROS), nitric oxide (NO), prostaglandin E2 (PGE2), pro‐inflammatory cytokines (IL‐6, IL‐1β, and TNF‐α), and nuclear factor‐kappa B (NF‐κB) protein expressions through western blotting. The results showed that LPS significantly activated the BV2 cells via toll‐like receptor 4 to induce elevated productions of ROS, NO, PGE2, IL‐6, and IL‐1β. However, tannic acid was able to reverse all the neuroinflammatory effects of LPS‐induced BV2 cells in a dose‐dependent manner. Collectively, the anti‐inflammatory effects of tannic acid on LPS‐induced BV2 microglial cells are attributed to the inhibition of ROS formation and the suppression of NF‐κB pathway activation. Tannic acid could be a potential therapeutic agent for the treatment of neurological related disorders.

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“…AD causes epidermal thickness with cutaneous hypersensitivity associated with increased serum immunoglobulin E (IgE) levels and infiltration of inflammatory cell types including mast cells and eosinophils [1–3]. …”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Yang

Yoo

Cho

et al. 2016

BMC Complement Altern Med

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BackgroundSanguisorbae Radix (SR) is a well-known herbal medicine used to treat inflammatory disease and skin burns in Asia. In addition, it is used to treat many types of allergic skin diseases, including urticaria, eczema, and allergic dermatitis. SR has been reported to exhibit anti-wrinkle, anti-oxidant, and anti-contact dermatitis bioactivities.MethodsIn this study, we investigated the mechanism underlying the anti-inflammatory effects of SR water extract (WSR) using human keratinocyte (HaCaT) cells and BALB/c mouse bone marrow-derived mast cells (BMMCs). Viability assays were used to evaluate non-cytotoxic concentrations of WSR in both BMMCs and HaCaT cells. To investigate the effect of WSR treatment on the degranulation of IgE/Ag-activated BMMCs, we measured the release of β-hexosaminidase (β-HEX). We determined the production of pro-inflammatory chemokines including thymus and activation regulated chemokine (TARC; CCL17), regulated on activation, normal T-cell expressed and secreted (RANTES; CCL5), macrophage-derived chemokine (MDC; CCL22), and interleukin 8 (IL-8; CXCL8) in stimulated human keratinocytes. The ability of WSR to reduce the expression of pro-inflammatory marker proteins was evaluated by Western blotting in HaCaT cells stimulated with tumor necrosis factor (TNF)-α/interferon (IFN)-γ.ResultWSR inhibited IgE/Ag-activated mast cell degranulation in BMMCs. Treatment with various concentrations of WSR decreased β-HEX release in a dose-dependent manner with an IC50 of 27.5 μg/mL. In keratinocytes, WSR suppressed TNF-α/IFN-γ-induced chemokine production and pro-inflammatory molecules via a blockade STAT-1, Jak-2, p38, and JNK activation.ConclusionsThis results demonstrate that WSR inhibits degranulation of IgE/Ag-activated mast cells and inhibits the production of pro-inflammatory chemokines by suppressing the phosphorylation of p38 and JNK in HaCaT cells.

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Tannic acid modulates NFκB signaling pathway and skin inflammation in NC/Nga mice through PPARγ expression

Cited by 54 publications

References 42 publications

Aqueous extract of Sanguisorba officinalis blocks the Wnt/β-catenin signaling pathway in colorectal cancer cells

Aqueous extract of Sanguisorba officinalis blocks the Wnt/β-catenin signaling pathway in colorectal cancer cells

Anti‐neuroinflammatory effects of tannic acid against lipopolysaccharide‐induced BV2 microglial cells via inhibition of NF‐κB activation

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

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