Anti‐neuroinflammatory effects of tannic acid against lipopolysaccharide‐induced BV2 microglial cells via inhibition of NF‐κB activation

Abstract

Microglia mediated neuroinflammation is known to cause various neurodegenerative and neurological ailments. Tannic acid is a natural polyphenol which has been reported to possess antioxidant, anti‐inflammatory, anticarcinogenic, antimutagenic, antitumor, and antimicrobial activities. As there are no reports till date on the anti‐neuroinflammatory effects of tannic acid, this study was conducted to analyze the possible mechanism and pathway involved in the prevention of neuroinflammation by tannic acid in BV2 microglial cells. BV2 microglial cells were pretreated with tannic acid (10, 25, and 50 μM/mL) and induced with lipopolysaccharide (LPS; 1 μM/mL) to assess the production of reactive oxygen species (ROS), nitric oxide (NO), prostaglandin E2 (PGE2), pro‐inflammatory cytokines (IL‐6, IL‐1β, and TNF‐α), and nuclear factor‐kappa B (NF‐κB) protein expressions through western blotting. The results showed that LPS significantly activated the BV2 cells via toll‐like receptor 4 to induce elevated productions of ROS, NO, PGE2, IL‐6, and IL‐1β. However, tannic acid was able to reverse all the neuroinflammatory effects of LPS‐induced BV2 cells in a dose‐dependent manner. Collectively, the anti‐inflammatory effects of tannic acid on LPS‐induced BV2 microglial cells are attributed to the inhibition of ROS formation and the suppression of NF‐κB pathway activation. Tannic acid could be a potential therapeutic agent for the treatment of neurological related disorders.

Antioxidant Nanoparticles for Concerted Inhibition of α-Synuclein Fibrillization, and Attenuation of Microglial Intracellular Aggregation and Activation

“…Although tannic acid treatment reduced nitrite production by 38% from αSYN-challenged microglia, this was not a statistically significant decrease, which could be due to the nitrite concentration approaching the detection limit of the Griess Reagent (2.5 µM) ( Figure 5A). While previous research has shown that tannic acid can reduce the production of neuroinflammatory factors such as ROS, NO and TNF-α from LPS-treated microglia via the suppression of the NF-κB pathway, our results show that tannic acid only reduces intracellular ROS production but does not have an appreciable effect on the other two markers (Figures 5A-D; Wu et al, 2019). This could potentially suggest that the rate at which tannic acid associates with αSYN is much faster than its interaction with other signaling molecules involved in the pathway (Shaham-Niv et al, 2018).…”

Tannylated Calcium Carbonate Materials with Antacid, Anti-Inflammatory, and Antioxidant Effects

“…In addition to antioxidant effects, TA molecules also exhibit anti-inflammatory properties [ 41 ]. We examined the in vitro anti-inflammatory effects of TA-CaCO 3 by analyzing the mRNA expression of pro-inflammatory factors, such as cyclooxygenase-2 (COX-2), interleukin (IL)-1β, IL-6, matrix metalloproteinase (MMP)-3, MMP-13, and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-stimulated chondrocytes, because LPS treatment leads to the overexpression of these pro-inflammatory factors [ 41 , 42 , 43 ].…”

<i>Spirogyra neglecta</i> Aqueous Extract Attenuates LPS-Induced Renal Inflammation

“…58) Similarly, catechin, gallic acid, rutin and quercetin also reduce inflammatory marker in this model. [58][59][60] Moreover, kaempferol also inhibits hepatocyte apoptosis in d-GalN/LPS-induced acute liver failure mice 61) while tannic acid reduces NF-κB expression in LPS-induced BV2 microglial cells. 62) Furthermore, polyphenol extract also showed anti-inflamatory effect by reducing plasma LPS and IL-6 levels in human volunteers.…”