Tannic acid inhibits lipid metabolism and induce ROS in prostate cancer cells

Nagesh, Prashanth K.B.; Chowdhury, Pallabita; Hatami, Elham; Jain, Shashi; Dan, Nirnoy; Kashyap, Vivek K.; Chauhan, Subhash C.; Jaggi, Meena; Yallapu, Murali M.

doi:10.1038/s41598-020-57932-9

Cited by 44 publications

(34 citation statements)

References 83 publications

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“…Although the present study indicated that TA attenuated ROS, ERS and apoptosis in LPS-induced H9C2 cells, there are discrepancies compared with other papers on different cell types; for example, in a previous study on prostate cancer cells, it was revealed that TA induced apoptosis, particularly by mediating ERS (61). TA also induces apoptosis by mediating ROS (62). The present study did not assess other cell models and therefore it is unclear whether TA can protect numerous cell types from apoptosis.…”

Section: Discussioncontrasting

confidence: 93%

Tannic acid alleviates lipopolysaccharide‑induced H9C2 cell apoptosis by suppressing reactive oxygen species‑mediated endoplasmic reticulum stress

et al. 2021

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Sepsis-induced myocardial dysfunction is one of the features of multiple organ dysfunction in sepsis, which is associated with extremely high mortality and is characterized by impaired myocardial compliance. To date, there are few effective treatment options available to cure sepsis. Tannic acid (TA) is reportedly protective during sepsis; however, the underlying mechanisms by which TA protects against septic heart injury remain elusive. The present study investigated the potential effects and underlying mechanisms of TA in alleviating lipopolysaccharide (LPS)-induced H9C2 cardiomyocyte cell apoptosis. H9C2 cells were treated with LPS (15 µg/ml), TA (10 µM) and TA + LPS; control cells were treated with medium only. Apoptosis was measured using flow cytometry, reverse transcription-quantitative PCR (RT-qPCR) and western blot analysis. Additionally, the levels of cellular reactive oxygen species (ROS), malondialdehyde and nicotinamide adenine dinucleotide phosphate were evaluated. Western blotting and RT-qPCR were also employed to detect the expression levels of endoplasmic reticulum (ER) stress-associated functional proteins. The present findings demonstrated that TA reduced the degree of LPS-induced H9C2 cell injury, including inhibition of ROS production and ER stress (ERS)-associated apoptosis. ERS-associated functional proteins, including activating transcription factor 6, protein kinase-like ER kinase, inositol-requiring enzyme 1, spliced X box-binding protein 1 and C/EBP-homologous protein were suppressed in response to TA treatment. Furthermore, the expression levels of ERS-associated apoptotic proteins, including c-Jun N-terminal kinase, Bax, cytochrome c, caspase-3, caspase-12 and caspase-9 were reduced following treatment with TA. Additionally, the protective effects of TA on LPS-induced H9C2 cells were partially inhibited following treatment with the ROS inhibitor N-acetylcysteine, which demonstrated that ROS mediated ERS-associated apoptosis and TA was able to decrease ROS-mediated ERS-associated apoptosis. Collectively, the present findings demonstrated that the protective effects of TA against LPS-induced H9C2 cell apoptosis may be associated with the amelioration of ROS-mediated ERS. These findings may assist the development of potential novel therapeutic methods to inhibit the progression of myocardial cell injury.

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Section: Discussioncontrasting

confidence: 93%

Tannic acid alleviates lipopolysaccharide‑induced H9C2 cell apoptosis by suppressing reactive oxygen species‑mediated endoplasmic reticulum stress

et al. 2021

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“…The broad cancer chemopreventive activity of various tannins in several animal models has been reviewed by Nepka et al [33]. In addition, recent studies have shown the anticancer efficacy of tannins, particularly tannic acid, against prostate and lung cancers [34,35]. The anticancer potentiality of doum palm extracts could be attributed to the high content of individual polyphenols, such as protocatechuic, p-hydroxybenzoic, chlorogenic, rosmarinic, vanillic, p-coumaric acids, apigenin-7-glucoside, rutin and chrysin.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Profiling and Antioxidant, Anticancer and Antimicrobial Activities of Hyphaene thebaica

et al. 2020

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This study investigated the metabolic content and biological activities of Hyphaene thebaica leaves, male parts and fruits methanolic extracts. The phytochemical constituents were determined, and multivariate data analysis discriminated the evaluated three parts into three groups according to the content of phenolics, flavonoids, flavonols, saponins, anthocyanins and tannins. High-performance liquid chromatography (HPLC) profiling of polyphenols revealed the dominance of catechins, rutin and apigenin-7-glucosides in leaves, protocatechuin, p-hydroxybenzoic, syringic, vanillic, rosmarinic, p-coumaric acids and chrysin in male parts, and chlorogenic acid in fruits. Leaves and male parts showed stronger free radical scavenging activity than the fruits. Positive correlations between total antioxidant capacity and carbohydrates, phenolics and flavonols were observed. The three extracts exhibited potent anti-cancer activity against liver and lung carcinoma cell lines. All extracts exhibited antibacterial activity, while only fruits showed antifungal efficacy. In conclusion, H. thebaica leaves, male parts and fruits contain a variety of phytochemicals with antioxidant, anticancer and antimicrobial activities.

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“…Tannins (commonly referred to as tannic acids, TA) are a class of polyphenolic compounds that are found in a variety of plant-based foods, especially berries, red wine, coffee, nuts and beans. TA is a powerful antioxidant, and therefore exhibits various pharmacological properties such as anti-toxic, anticancer, antiallergic, antiviral and antibacterial abilities [3][4][5][6]. It is thought that TA could protect leaves against insects by deterrence and/or toxicity and induce reactive oxygen species (ROS) development in prostate cancer cells by inhibiting lipid metabolism [3,7].…”

Section: Introductionmentioning

confidence: 99%

Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs

et al. 2021

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To investigate the effects of tannins (TA) on porcine oocyte in vitro maturation (IVM), different concentrations of TA (0, 1, 10 and 100 μg/mL) were supplemented with a maturation medium and the COCs and subsequent embryonic development were examined. The results showed that 10 µg/mL TA significantly improved the cumulus expansion index (CEI), cumulus-expansion-related genes (PTGS1, PTGS2, PTX-3, TNFAIP6 and HAS2) expression and blastocyst formation rates after parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) compared to the control groups, but not oocyte nuclear maturation. Nevertheless, 10 µg/mL TA dramatically enhanced the mRNA expression of oocyte-development-related genes (BMP15, GDF9, CDC2 and CYCLIN B1), GSH, ATP, SOD1, PGC1α, BMP15, GDF9 and CDC2 levels and reduced intracellular ROS level in porcine oocytes. These results indicated that porcine oocyte cytoplasmic maturation was improved by 10 µg/mL TA treatment during IVM. In contrast, a high concentration of TA (100 μg/mL) significantly decreased the CEI and PTGS1, PTGS2, PTX-3 and HAS2 mRNA expressions in cumulus cells, and reduced oocyte nuclear maturation and the total cell numbers/blastocyst. In general, these data showed that 10 μg/mL TA supplementation has beneficial effects on oocyte cytoplasmic maturation and subsequent embryonic development in pigs.

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Tannic acid inhibits lipid metabolism and induce ROS in prostate cancer cells

Cited by 44 publications

References 83 publications

Tannic acid alleviates lipopolysaccharide‑induced H9C2 cell apoptosis by suppressing reactive oxygen species‑mediated endoplasmic reticulum stress

Tannic acid alleviates lipopolysaccharide‑induced H9C2 cell apoptosis by suppressing reactive oxygen species‑mediated endoplasmic reticulum stress

Metabolomic Profiling and Antioxidant, Anticancer and Antimicrobial Activities of Hyphaene thebaica

Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs

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