Tannic acid-crosslinked chitosan matrices enhance osteogenic differentiation and modulate epigenetic status of cultured cells over glutaraldehyde crosslinking

Acharya, Veena; Ghosh, Aritri; Chowdhury, Amit Roy; Datta, Pallab

doi:10.1080/1539445x.2021.1933032

Cited by 14 publications

(7 citation statements)

References 50 publications

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“…These observations are related to the fact that TA can interact with CS, G and Fe. All these different interactions, ionic, hydrogen or covalent bonds result in more crosslinking and more trapped G and, thus, less mass loss within the first 24 h. Acharya et al, also noticed that their CS scaffold degradation decreased from 35% to 20% after crosslinking with TA [ 21 ], values close to those observed herein.…”

Section: Resultssupporting

confidence: 82%

“…These data clearly demonstrate that TA induces crosslinking of CS, which is in agreement with the literature. For instance, Acharya et al, showed that by adding TA to CS, the percentage of scaffold swelling decreases from 250% to 100%, i.e., twofold less [ 21 ], whereas in this study, it is fourfold less. This difference can be explained by CS characteristics, such as deacetylation degree and molecular weight, as well as the amount of TA added.…”

Section: Resultscontrasting

confidence: 55%

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Prolonged Antibacterial Activity in Tannic Acid–Iron Complexed Chitosan Films for Medical Device Applications

et al. 2023

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Healthcare-associated infections (HAIs) represent a global burden, leading to significant mortality and generating financial costs. One important cause of HAIs is the microbiological contamination of implantable medical devices. In this context, a novel antimicrobial drug-eluting system, based on chitosan and loaded with gentamicin, a broad-spectrum antibiotic, was developed. The effects of the addition of tannic acid and different FeSO4 concentrations on the loaded antibiotic release were evaluated. The properties of the films were assessed in terms of thickness, swelling, mass loss and wettability. The films’ surface composition was characterized by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy. The antibiotic release in phosphate buffer saline was quantified by high-performance liquid chromatography–mass spectrometry, and the antibacterial activity was evaluated. Hemolysis and cytotoxicity were also assessed. The results showed that the addition of tannic acid and iron decreased the swelling degree and degradation due to strong interactions between the different components, thus impacting gentamicin release for up to 35 days. In conclusion, this study presents a novel strategy to produce low-cost and biocompatible antimicrobial drug-eluting systems with sustained and prolonged antibacterial activity over more than a month.

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Section: Resultssupporting

confidence: 82%

Section: Resultscontrasting

confidence: 55%

Prolonged Antibacterial Activity in Tannic Acid–Iron Complexed Chitosan Films for Medical Device Applications

et al. 2023

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“…The main TMP peaks are assigned as follows: at 3,469 and 3,317 cm −1 to N–H stretching vibration, from 1,653 to 1,631 cm −1 to amine bending, from 1,458 cm −1 to C=C aromatic stretching, at 1,235 and 1,124 cm −1 to C–N and C–O stretching, respectively, and at 830 cm −1 to C–H bending from the substituted aromatic ring ( Ungurean et al, 2013 ; Da Silva et al, 2016 ). The TMP peaks are visible on the CS/TMP film, without a clear difference, except a broadening of the area of H bonding in 3,200–3,600 cm −1 , which may be due to the formation of additional hydrogen bonds as reported by Acharya et al (2021) , whereas on films with quercetin, TMP peaks are less evident, and some band shifts are observed, in particular the band at 1,660 cm −1 . Indeed, this band was neither detected before on CS/Q films nor on TMP or chitosan films.…”

Section: Resultsmentioning

confidence: 54%

Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs

Wiggers

Chevallier

Copes

et al. 2022

Front. Bioeng. Biotechnol.

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Natural polymer-based films, due to their favorable biological and mechanical properties, have demonstrated great potential as coatings for biomedical applications. Among them, chitosan films have been widely studied both as coating materials and as controlled drug release systems. Crosslinkers are often used to tune chitosan’s crosslinking degree and thus to control the drug release kinetics. For this purpose, quercetin, a plant-derived natural polyphenol, has gained attention as a crosslinker, mainly for its intrinsic anti-inflammatory, antioxidant, and antibacterial features. In this study, chitosan films crosslinked with three different concentrations of quercetin (10, 20, and 30% w/w) have been used as controlled release systems for the delivery of the antibacterial drug trimethoprim (TMP, 10% w/w). Physicochemical and antimicrobial properties were investigated. Surface wettability and composition of the films were assessed by contact angle measurements, X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared spectroscopy (FTIR), respectively. The release kinetic of TMP in phosphate-buffered saline (PBS) and 2-(N-morpholino) ethanesulfonic acid (MES) was studied over time. Finally, antibacterial properties were assessed on E. coli and S. aureus through Kirby–Bauer disc diffusion and micro-dilution broth assays. Results show that quercetin, at the tested concentrations, clearly increases the crosslinking degree in a dose-dependent manner, thus influencing the release kinetic of the loaded TMP while maintaining its bactericidal effects. In conclusion, this work demonstrates that quercetin-crosslinked chitosan films represent a promising strategy for the design of antibiotic-releasing coatings for biomedical applications.

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“…Ultrastructural analyses have indicated tunable structure via the regulation of cross-linkers content and variants [ 150 , 151 ]. To date, several cross-linker types such as epichlorohydrin, glyoxal glutaraldehyde, formaldehyde, and ethylene glycol diglycidyl ether (EDGE) have been used for the fabrication of chemically cross-linked hydrogels [ 152 – 156 ]. In vitro and in vivo analyses have shown various degrees of mutagenicity, calcification, and cytotoxicity after being implanted into the target tissues [ 157 , 158 ].…”

Section: Chemical and Non-chemical Cross-linking In Scaffold Synthesismentioning

confidence: 99%

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

et al. 2023

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Muscular diseases and injuries are challenging issues in human medicine, resulting in physical disability. The advent of tissue engineering approaches has paved the way for the restoration and regeneration of injured muscle tissues along with available conventional therapies. Despite recent advances in the fabrication, synthesis, and application of hydrogels in terms of muscle tissue, there is a long way to find appropriate hydrogel types in patients with congenital and/or acquired musculoskeletal injuries. Regarding specific muscular tissue microenvironments, the applied hydrogels should provide a suitable platform for the activation of endogenous reparative mechanisms and concurrently deliver transplanting cells and therapeutics into the injured sites. Here, we aimed to highlight recent advances in muscle tissue engineering with a focus on recent strategies related to the regulation of vascularization and immune system response at the site of injury.

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Tannic acid-crosslinked chitosan matrices enhance osteogenic differentiation and modulate epigenetic status of cultured cells over glutaraldehyde crosslinking

Cited by 14 publications

References 50 publications

Prolonged Antibacterial Activity in Tannic Acid–Iron Complexed Chitosan Films for Medical Device Applications

Prolonged Antibacterial Activity in Tannic Acid–Iron Complexed Chitosan Films for Medical Device Applications

Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

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