Tandem catalysis in multicomponent solvent-free biofluids

Abstract

The core–shell architecture of biohybrid enzymes facilitates construction of multifunctional biofluids which display extremophilic traits in total absence of solvent.

Single Enzyme Nanoparticles with Improved Biocatalytic Activity through Protein Entrapment in a Surfactant Shell

“…For covalently conjugated biohybrids, the surfactants glycolic acid ethoxylate lauryl ether (C 12 EO 10 ), or carboxylated Brij L23 (C 12 EO 22 ) were dissolved in buffer to achieve a final concentration of 20 mg mL –1 . To activate the carboxylic acid, solid N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride (EDC, 104 μmol) was mixed with the surfactant solution (34 μmol) and was allowed to stir for 30 min.…”

“…Recently, amphiphiles with a block alkyl-glycolic acid ethoxylate (alkyl-EO) architecture have emerged as exciting targets for the discrete nanoencapsulation of single enzymes. − This is owed to their nature to self-assemble, which offers a means to stabilize proteins in new environments. For example, in the total absence of a solvent, protein–surfactant nanoconjugates display thermotropic behavior and hyperthermal stability. − A surfactant corona also facilitates stabilization in a range of solvents such as organic solvents and ionic liquids. ,, The multistep preparation of electrostatically assembled SENs from enzymes and surfactants generally consists of chemical modification of solvent-accessible acidic residues (cationic supercharging of Asp/Glu) followed by electrostatic conjugation of anionic surfactant to coat the protein surface.…”

On Complex Coacervate Core Micelles: Structure-Function Perspectives

“…The high thermal stability has been exploited to perform enzymatic reactions at exceptionally high temperatures. For example, cascade reactions between HRP, GOx, and lipase were executed at temperatures up to 140 °C, in the absence of solvent ( Figure 5 ) [ 128 ]. Interestingly, this multicomponent system was inactive below the melting transition of the biohybrids (ca.…”

“…( b ) Conversion of o-phenylenediamine to 2,3-diaminophenazine by HRP during the cascade reaction at temperatures > 80 °C in the absence of solvent. Adapted from Atkins et al (doi:10.1039/c9nr06045f) as published by the Royal Society of Chemistry [ 128 ].…”

Preparation and application of solvent-free liquid proteins with enhanced thermal and anhydrous stabilities

“…showed that high thermal stability and enzyme activity could be conferred onto a mix of enzymes in a multicomponent solventfree liquid. 69 In this work the authors made a solvent-free liquid containing horseradish peroxidase, glucose oxidase, and lipase that was able to form 2,3-diaminophenazine from ophenylenediamine mediated by a cascade reaction starting with acetylated glucose (Fig. 2e).…”