Tamoxifen induces suppression of cell viability and apoptosis in the human hepatoblastoma cell line HepG2 via down‐regulation of telomerase activity

Brandt, Sebastián; Heller, H.; Schuster, K.-D.; Grote, J.

doi:10.1111/j.1478-3231.2004.00887.x

Cited by 27 publications

(24 citation statements)

References 37 publications

(51 reference statements)

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“…has been described for a number of chemotherapeutic drugs such as tamoxifen [40], doxorubicin and vincristine [41]. Kobayashi et al [41] demonstrated that at high cell densities, a decreased cellular uptake of chemotherapeutics results in attenuated availability 19 of drug molecules at its intracellular binding sites.…”

Section: Discussionmentioning

confidence: 99%

Cannabidiol inhibits cancer cell invasion via upregulation of tissue inhibitor of matrix metalloproteinases-1

Ramer

Merkord

Rohde

et al. 2010

Biochemical Pharmacology

151

149

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Although cannabinoids exhibit a broad variety of anticarcinogenic effects, their potential use in cancer therapy is limited by their psychoactive effects. Here we evaluated the impact of cannabidiol, a plant-derived non-psychoactive cannabinoid, on cancer cell invasion. Using Matrigel invasion assays we found a cannabidiol-driven impaired invasion of human cervical cancer (HeLa, C33A) and human lung cancer cells (A549) that was reversed by antagonists to both CB 1 and CB 2 receptors as well as to transient receptor potential vanilloid 1 (TRPV1). Additionally, in vivo studies in thymic-aplastic nude mice revealed a significant inhibition of A549 lung metastasis in cannabidiol-treated animals as compared to vehicle-treated controls.Altogether, these findings provide a novel mechanism underlying the anti-invasive action of cannabidiol and imply its use as a therapeutic option for the treatment of highly invasive cancers. Page 3 of 40A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

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Section: Discussionmentioning

confidence: 99%

Cannabidiol inhibits cancer cell invasion via upregulation of tissue inhibitor of matrix metalloproteinases-1

Ramer

Merkord

Rohde

et al. 2010

Biochemical Pharmacology

151

149

View full text Add to dashboard Cite

show abstract

“…In addition, while the rate of apoptotic nuclei was still low (about 5%) following 24 hour incubation of the cells, it increased up to 63% when the incubation time was prolonged to 72 hours (33). In another study which was carried out by the same researchers, it has shown that tamoxifen does not affect the transcription level olf hRT, hTERT, and TP1 in HepG2 cells.…”

Section: Discussionmentioning

confidence: 90%

Evaluation Of Effects Of Quercetin (3,3',4',5,7-pentohidroxyflavon) On Apoptosis And Telomerase Enzyme Activity In Mcf-7 And Nih-3t3 Cell Lines To Compared With Tamoxifen

Hatipoglu¹,

Başaran²,

Dikmen³

et al. 2010

TUTFD

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Objective: Quercetin has been shown to inhibit the proliferation of cancer cells. Tamoxifen is used for breast cancer. In this study, we have aimed to investigate the effects of quercetin and tamoxifen on telomerase enzyme activity and apoptosis in two cell lines. Methods:In this study, 10, 50 and 100 µM of quercetin and tamoxifen were used to treat MCF-7 and NIH-3T3. Apoptosis was determined by the TUNEL method (Terminal Deoxynucleotide Transferase dUTP Nick End Label) and telomerase enzyme activity was determined by ELISA (Enzyme-Linked Immunosorbent Assay).Results: In the NIH3T3 cell line, only 100 µM quercetin and tamoxifen induced significant apoptosis. In the MCF-7 cell line 10 µM and 100 µM quercetin in the 24 th hour and 100 µM quercetin in the 72 nd hour induce apoptosis. In 24 th and 48 th hours, 50 µM tamoxifen and 100 µM tamoxifen in the 24 th and 48 th hours induce apoptosis in the MCF-7 cell line. In MCF-7 and NIH-3T3 cell lines, all doses of quercetin and tamoxifen reduced telomerase enzyme activity compared to the control group. Conclusion:In this study, it was shown that quercetin has similar effects to tamoxifen. However quercetin induces apoptosis more than decreasing telomerase enzyme activities, being different from tamoxifen. We hope that the findings will assist in developing new therapeutic pathways for preventing breast cancer. However, there should be many more studies in order to discover quercetin and other potential drugs.

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“…The mechanisms of tamoxifen action in HCC, however, are not yet clearly understood. Results from studies on the human hepatoblastoma cell line HepG2 provide evidence that estrogen-receptor-a-independent antiproliferative actions of tamoxifen in HCC are mediated by the suppression of telomerase activity [5]. Materials and methods: We investigate the pathway of the tamoxifen-induced down-regulation of telomerase activity, using HepG2 cells incubated over 24 h or 48 h in the presence of 20 lM tamoxifen.…”

mentioning

confidence: 99%

The tamoxifen-induced suppression of telomerase activity in the human hepatoblastoma cell line HepG2: a result of post-translational regulation

Brandt

Heller

Schuster

et al. 2004

J Cancer Res Clin Oncol

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Tamoxifen induces suppression of cell viability and apoptosis in the human hepatoblastoma cell line HepG2 via down‐regulation of telomerase activity

Abstract: The tamoxifen-induced reduction of cell viability in HepG2 cells depends on drug concentration and cell density and is due to cytostatic and cytocide effects. The latter may be mediated by a down-regulation of telomerase activity.

Cited by 27 publications

References 37 publications

Cannabidiol inhibits cancer cell invasion via upregulation of tissue inhibitor of matrix metalloproteinases-1

Cannabidiol inhibits cancer cell invasion via upregulation of tissue inhibitor of matrix metalloproteinases-1

Evaluation Of Effects Of Quercetin (3,3',4',5,7-pentohidroxyflavon) On Apoptosis And Telomerase Enzyme Activity In Mcf-7 And Nih-3t3 Cell Lines To Compared With Tamoxifen

The tamoxifen-induced suppression of telomerase activity in the human hepatoblastoma cell line HepG2: a result of post-translational regulation

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