Talaromyces pinophilus strain M13: a portrayal of novel groundbreaking fungal strain for phytointensification

Patel, Dhavalkumar; Patel, Abhishek; Patel, M.A. Patel M.G.; Goswami, Dweipayan

doi:10.1007/s11356-020-11152-w

Cited by 12 publications

(12 citation statements)

References 39 publications

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“…Afterwards, a phylogenetic tree was constructed with the help of MEGA 7.0 [ 53 ] by measuring distances and clustering using the Maximum Likelihood strategy of [ 54 ]. The parameter chosen for the phylogenetic tree construction was Bootstrap values based on 1000 replications [ 55 ], and all branches with less than 50% site coverage were collapsed. Fusarium verticillioides and Fusarium subglutinans were selected as the out-group species.…”

Section: Methodsmentioning

confidence: 99%

“…This fungal species has been widely employed as effective cellulose and waste degrading agent [ 8 , 9 ], a renewable source of natural colourants [ 10 ], and a stimulator of phytoremediation efficacy [ 11 ]. Furthermore, T. pinophilus exhibited plant development-promoting properties on Waito-C rice [ 12 ] and chickpea [ 13 ], as well as mycoparasitic activity against Botrytis cinerea [ 14 ] and Rhizoctonia solani [ 15 ]. Interestingly, 3-O-methylfunicone, a prominent metabolite of T. pinophilus strain F36CF has been shown to have insecticidal activity against aphids [ 7 ] and antiviral effect on Bovine Herpesvirus 1 infection [ 16 ], while 2-hydroxyradiclonic acid, a methanolic extract of T. pinophilus strain AF-02 obtained from a Chinese green onion, demonstrated potent antibacterial activity against Escherichia coli [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Variability in metabolites produced by Talaromyces pinophilus SPJ22 cultured on different substrates

Adelusi

Gbashi

Adebiyi

et al. 2022

Fungal Biol Biotechnol

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Background Several metabolites released by fungal species are an essential source of biologically active natural substances. Gas chromatography high resolution time-of-flight mass spectrometry (GC-HRTOF-MS) is one of the techniques used in profiling the metabolites produced by microorganisms, including Talaromyces pinophilus. However, there is limited information regarding differential substrates’ impacts on this fungal strain’s metabolite profiling. This study examined the metabolite profile of T. pinophilus strain SPJ22 cultured on three different media, including solid czapek yeast extract agar (CYA), malt extract agar (MEA) and potato dextrose agar (PDA) using GC-HRTOF-MS. The mycelia including the media were plugged and dissolved in 5 different organic solvents with varying polarities viz.: acetonitrile, dichloromethane, hexane, 80% methanol and water, and extracts analysed on GC-HRTOF-MS. Results The study revealed the presence of different classes of metabolites, such as fatty acids (2.13%), amides (4.26%), alkanes (34.04%), furan (2.13%), ketones (4.26%), alcohols (14.89%), aromatic compounds (6.38%), and other miscellaneous compounds (17.02%). Significant metabolites such as acetic acid, 9-octadecenamide, undecanoic acid methyl ester, hydrazine, hexadecane, nonadecane, eicosane, and other compounds reported in this study have been widely documented to have plant growth promoting, antimicrobial, anti-inflammatory, antioxidant, and biofuel properties. Furthermore, T. pinophilus grown on PDA and MEA produced more than twice as many compounds as that grown on CYA. Conclusion Thus, our result showed that the production of essential metabolites from T. pinophilus is substrate dependent, with many of these metabolites known to have beneficial characteristics, and as such, this organism can be utilised as a sustainable and natural source for these useful organic molecules.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variability in metabolites produced by Talaromyces pinophilus SPJ22 cultured on different substrates

Adelusi

Gbashi

Adebiyi

et al. 2022

Fungal Biol Biotechnol

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“…forms the good Fig. 6 a Interaction profile of crucial interacting amino acids of the SARS-CoV2-PLpro in contact with GRL0617 b GRL0617 Ligand interaction diagram displaying total time (in %) a particular amino acid of the protein over the course of simulation part of microbes [32,33]. The bad is the crop spoilage by fungi and other saprophytes while the glimpse of the ugly side is the current COVID-19 pandemic that we are experiencing [6].…”

Section: Discussionmentioning

confidence: 99%

“…Humans exploit microbes to produce alcoholic beverages, antibiotics, flavoring agents etc. forms the good part of microbes [ 32 , 33 ]. The bad is the crop spoilage by fungi and other saprophytes while the glimpse of the ugly side is the current COVID-19 pandemic that we are experiencing [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Meticulous assessment of natural compounds from NPASS database for identifying analogue of GRL0617, the only known inhibitor for SARS-CoV2 papain-like protease (PLpro) using rigorous computational workflow

et al. 2021

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The latest global outbreak of 2019 respiratory coronavirus disease (COVID-19) is triggered by the inception of novel coronavirus SARS-CoV2. If recent events are of any indicators of the epidemics of past, it is undeniable to state a fact that the SARS-CoV2 viral infection is highly transmissible with respect to its previously related SARS-CoV's. Papain-like protease (PLpro) is an enzyme that is required by the virus itself for replicating into the host system; and it does so by processing its polyproteins into a functional replicase complex. PLpro is also known for downregulating the genes responsible for producing interferons, an essential family of molecules produced in response to viral infection, thus making this protein an indispensable drug target. In this study, PLpro inhibitors were identified through high throughput structure-based virtual screening approach from NPASS natural product library possessing ~ 35,000 compounds. Top five hits were scrutinised based on structural aromaticity and ability to interact with a key active site residue of PLpro, Tyr268. For second level of screening, the MM-GBSA End-Point Binding Free Energy Calculation of the docked complexes was performed, which identified Caesalpiniaphenol A as the best hit. Caesalpiniaphenol A not only possess a double ring aromatic moiety but also has lowest minimum binding energy, which is at par with the control GRL0617, the only known inhibitor of SARS-CoV2 PLpro. Details of the Molecular Dynamics (MD) simulation and ADMET analysis helped to conclusively determine Caesalpiniaphenol A as potentially an inhibitor of SARS-CoV2 PLpro.

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“…Species such as T. flavus, T. pinophilus and T. purpureogenus are already known to be producers of bioactive compounds, playing a fundamental role in antagonism exerted against plant pathogens. Considering that these species are also increasingly reported as plant growth promoters [104], more accurate investigations concerning their anti-insectan aptitude might disclose additional positive effects on plant health and integrate the profile of multipurpose strains to be employed in crop management.…”

Section: Discussionmentioning

confidence: 99%

Talaromyces–Insect Relationships

Nicoletti

Becchimanzi

2021

Microorganisms

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Facing the urgent need to reduce the input of agrochemicals, in recent years, the ecological relationships between plants and their associated microorganisms have been increasingly considered as an essential tool for improving crop production. New findings and data have been accumulated showing that the application of fungi can go beyond the specific role that has been traditionally assigned to the species, employed in integrated pest management as entomopathogens or mycoparasites, and that strains combining both aptitudes can be identified and possibly used as multipurpose biocontrol agents. Mainly considered for their antagonistic relationships with plant pathogenic fungi, species in the genus Talaromyces have been more and more widely reported as insect associates in investigations carried out in various agricultural and non-agricultural contexts. Out of a total of over 170 species currently accepted in this genus, so far, 27 have been found to have an association with insects from 9 orders, with an evident increasing trend. The nature of their mutualistic and antagonistic relationships with insects, and their ability to synthesize bioactive compounds possibly involved in the expression of the latter kind of interactions, are analyzed in this paper with reference to the ecological impact and applicative perspectives in crop protection.

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Talaromyces pinophilus strain M13: a portrayal of novel groundbreaking fungal strain for phytointensification

Cited by 12 publications

References 39 publications

Variability in metabolites produced by Talaromyces pinophilus SPJ22 cultured on different substrates

Variability in metabolites produced by Talaromyces pinophilus SPJ22 cultured on different substrates

Meticulous assessment of natural compounds from NPASS database for identifying analogue of GRL0617, the only known inhibitor for SARS-CoV2 papain-like protease (PLpro) using rigorous computational workflow

Talaromyces–Insect Relationships

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