Taking the Starch out of Oral Biofilm Formation: Molecular Basis and Functional Significance of Salivary α-Amylase Binding to Oral Streptococci

Nikitkova, Anna E.; Haase, Elaine M.; Scannapieco, Frank A.

doi:10.1128/aem.02581-12

Cited by 56 publications

(67 citation statements)

References 55 publications

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“…As orthopedic devices find increasing applications, the incidence of infected implants also increases [192]. Infections associated with medical implants, Lancet mellitus, cystic fibrosis and nonhealing wounds are usually induced by bacterial biofilms [193][194][195]. The biofilm is a community of microorganisms attached to surface and surrounded by extracellular matrix, Fig.…”

Section: Nitric Oxide As Antimicrobial Agentmentioning

confidence: 99%

Nitric oxide based strategies for applications of biomedical devices

Yang

et al. 2015

Biosurface and Biotribology

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Since the first report describing nitric oxide (NO) in the mid-1980s, research efforts have been devoted to elucidating the pathway of NO generation and the mechanism of NO function in vivo. The worldwide expansion of NO investigations have helped advance progress in biomedical device applications of NO-based strategies for cardiovascular devices, wound healing and antimicrobial agents, including recent in vivo investigations. Here we describe the general concepts of NO discovery, mechanisms of NO synthesis in vivo and NO producing materials. Both design strategies and results are discussed and compared in release kinetics, NO dose and biological effects, with the aim of providing foundations for the development of new NO-based therapeutics. & 2015 Southwest Jiaotong University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Section: Nitric Oxide As Antimicrobial Agentmentioning

confidence: 99%

Nitric oxide based strategies for applications of biomedical devices

Yang

et al. 2015

Biosurface and Biotribology

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“…For this purpose, we used a biofilm model that was developed to evaluate the cariogenic potential of starchy dietary products [Cavalcanti et al, 2014]. This biofilm is composed of S. mutans (the most cariogenic bacteria), Actinomyces naeslundii (which has amylolytic activity) [Glor et al, 1988], and Streptococcus gordonii (which binds salivary amylase) [Nikitkova et al, 2013].…”

mentioning

confidence: 99%

Starch Combined with Sucrose Provokes Greater Root Dentine Demineralization than Sucrose Alone

Souza¹,

Sampaio

Cury

et al. 2018

Caries Res

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Since there is no consensus about whether starch increases the cariogenic potential of sucrose, we used a validated 3-species biofilm model to evaluate if starch combined with sucrose provokes higher root dentine demineralization than sucrose alone. Biofilms (n = 18) composed by Streptococcus mutans (the most cariogenic bacteria), Actinomces naeslundii (which has amylolytic activity), and Streptococcus gordonii (which binds salivary amylase) were formed on root dentine slabs under exposure 8 ×/day to one of the following treatments: 0.9% NaCl, 1% starch, 10% sucrose, or a combination of 1% starch and 10% sucrose. Before each treatment, biofilms were pretreated with human whole saliva for 1 min. The pH of the culture medium was measured daily as an indicator of biofilm acidogenicity. After 96 h of growth, the biofilms were collected, and the biomass, bacteria viability, and polysaccharides were analyzed. Dentine demineralization was assessed by surface hardness loss (% SHL). Biofilm bioarchitecture was analyzed using confocal laser scanning microscopy. Treatment with a starch and sucrose combination provoked higher (p = 0.01) dentine demineralization than sucrose alone (% SHL = 53.2 ± 7.0 vs. 43.2 ± 8.7). This was supported by lower pH values (p = 0.007) of the culture medium after daily exposure to the starch and sucrose combination compared with sucrose (4.89 ± 0.29 vs. 5.19 ± 0.32). Microbiological and biochemical findings did not differ between biofilms treated with the combination of starch and sucrose and sucrose alone (p > 0.05). Our findings give support to the hypothesis that a starch and sucrose combination is more cariogenic for root dentine than sucrose alone.

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“…Salivary amylase is an abundant enzyme produced primarily from serous cells of the parotid. Human saliva contains on average 0.04 -0.4 mg/mL of amylase constituting up to 5% of the total salivary protein content [32]. The enzymatic activity of salivary alphaamylase protein is to hydrolyze food starch into maltose and glucose [33].…”

Section: Amylase I Genementioning

confidence: 99%

“…When tooth surfaces are initially exposed to the oral environment, a thin film absent of cells is formed called the acquired enamel pellicle (AEP) [32].…”

Section: Amylase I Genementioning

confidence: 99%

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Characterization of promoter regions of parotid gland genes.

Pérez¹

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Taking the Starch out of Oral Biofilm Formation: Molecular Basis and Functional Significance of Salivary α-Amylase Binding to Oral Streptococci

Cited by 56 publications

References 55 publications

Nitric oxide based strategies for applications of biomedical devices

Nitric oxide based strategies for applications of biomedical devices

Starch Combined with Sucrose Provokes Greater Root Dentine Demineralization than Sucrose Alone

Characterization of promoter regions of parotid gland genes.

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