“…In 1948, Cohn and Wolfson increased the final concentration of sodium sulphite to 26.9 % and found that the separation correlated well with electrophoresis. Phosphate salts also (Aull and McCord, 1955;Butler, Blatt and Southgate, 1935;Butler and Montgomery, 1932;Derrien, 1944;Wuhrmann and Leuthardt, 1938;Leuthardt, 1945;Roche and Derrien, 1945;Wuhrmann and Wunderly, 1952) have been employed for fractionation and offer the significant advantage of excellent pH control when used as buffer salts. Derrien (1944) was able to detect as many as twelve serum protein fractions by solubility curves with phosphates.…”