Tailoring the Surface Properties of Co-based Metal–Organic Frameworks for Highly Efficient and Selective Enrichment of Immunoglobulin G

Abstract

The high tunability of metal−organic frameworks (MOFs) provides attractive flexibility to tailor their surface properties for practical demands. Here we report the regulation of the surface properties (hydrophilicity and charge characteristics) of Co-based MOFs by exploiting different organic building units and tailor them as efficient adsorbents for specific protein enrichment. Compared with the pristine Co-based MOF (Co-MOF) and the aminated MOF (Co-MOF-NH 2 ), the MOF decorated with abundant hydroxyl groups (Co-MOF-OH) exhibits superior adsorption selectivity and enriched efficiency toward immunoglobulin G (IgG) in the physiological state (pH 7.4) by taking advantage of the favorable hydrogen-bonding interactions and electrostatic force between IgG and Co-MOF-OH. The enrichment factor for IgG is high, up to 97.7 for enriching IgG from the IgG/human serum albumin mixture with a mass ratio of 1:50, and circular dichroism indicates that the enrichment process poses no influence on the protein structure. Moreover, Co-MOF-OH proves its practicability in complex biological samples by the selective extraction of IgG from complex human serum samples.

An overview on the investigation of nanomaterials' effect on plasma components: immunoglobulins and coagulation factor VIII, 2010–2020 review

“…The result indicated that the structure of IgG did not experience any changes during the enrichment process and practicability of Co-MOF-OH in a complex biological solution was confirmed by the selective enrichment of IgG from human serum samples. 122 …”

Titanium‐based metal‐organic framework MIL‐125(Ti) for the highly selective isolation and purification of immunoglobulin G from human serum

“…By fitting the data into the following equation, the theoretical maximum adsorption capacities of IgG are concluded to be 232.56 mg/g. Table 1 summarizes the maximum capacity of some reported materials MEP HyperCel resin 10.4 [39] Fe 3 O 4 @SiO 2 @mesoporous SiO 2 microsphere 41 [40] [PHEMA/PEI]-Cu(II) 72.28 [41] Co-MOF-OH 97.7 [42] UiO@GO@PEG 139.6 the other stripping reagents mentioned herein give rise to virtually no recovery of the adsorbed IgG. 0.1% SDS solution is thus preferred for the elution of the adsorbed IgG in this case.…”

Copper ion based metal–organic framework nanomaterials with roughness enhanced protein adhesion for high-efficiency hemoglobin separation

“…[24][25][26][27] In terms of adsorption and separation applications, MOFs are widely used not only in the adsorption and separation of small molecules but also for selective enrichment and capture of biological macromolecules. [28][29][30] Compared with traditional materials, MOFs could be precisely regulated at the molecular level to achieve highly specific enrichment through rationally selecting organic ligands and metal cations. 31,32 In view of these excellent properties of the MOFs, great interest has been aroused in protein separation.…”