Copper ion based metal–organic framework nanomaterials with roughness enhanced protein adhesion for high-efficiency hemoglobin separation

He, Qiu-Ling; Jia, Ben-Xu; Wang, Yukun; Qin, Meng; Xu, Wang-Bo; Zhang, Zneng; Feng, Yi; Zhou, Bo

doi:10.1039/d2nj06115e

Cited by 9 publications

(2 citation statements)

References 49 publications

(56 reference statements)

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“…These problems ultimately lead to low adsorption performance and environmental pollution, which restrict their practical application. As an alternative strategy, magnetic composites incorporating the metal–organic framework (MOF), , layered double hydroxide (LDH), , and montmorillonite , are also reported for the separation of histidine-rich proteins. Despite the success of the aforementioned strategy in resolving the defects of ligand-based magnetic IMA adsorbents, there is a trade-off between magnetism and adsorption performance.…”

Section: Introductionmentioning

confidence: 99%

Sustainable and Well-Dispersed Cu-Doped Magnetic Vortex Nanorings for Highly Specific Separation of His-Rich Proteins

Wang,

Niu,

Wang

et al. 2024

ACS Appl. Nano Mater.

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The combination of magnetic nanomaterials with the immobilized metal affinity (IMA) technique has emerged as a powerful method to specifically separate histidine (His)-rich proteins. However, obtaining a high-performance and sustainable magnetic adsorbent with good dispersibility and minimal copper leakage remains a major challenge. Herein, we propose a simple ligand-free method to acquire Cu-doped magnetic nanorings (Fe 3 O 4 −Cu NR) for the highly selective enrichment of His-rich proteins. Importantly, the Fe 3 O 4 −Cu NR with unique ring-like structural features offers numerous exposed Cu 2+ binding sites for His-rich proteins while maintaining a high magnetic behavior (61.7 emu g −1 ). Thus, the Fe 3 O 4 −Cu NR exhibits a remarkable adsorption capacity (932.2 mg g −1 ) and high selectivity (SF < 0.1) for bovine hemoglobin (BHb), surpassing most of the reported IMA magnetic adsorbents. Moreover, Fe 3 O 4 −Cu NR can be easily regenerated with negligible copper ion leakage (<0.1 μmol L −1 ) and without requiring a reincubation step, demonstrating reusability for up to six cycles. In addition, the removal efficiency and recovery of Fe 3 O 4 −Cu NR for BHb in the diluted bovine blood were found to be 89.79 and 70.25%, respectively, further indicating the specificity of Fe 3 O 4 −Cu NR to BHb in complex biological samples.

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Section: Introductionmentioning

confidence: 99%

Sustainable and Well-Dispersed Cu-Doped Magnetic Vortex Nanorings for Highly Specific Separation of His-Rich Proteins

Wang,

Niu,

Wang

et al. 2024

ACS Appl. Nano Mater.

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“…The transition metal decoration on a variety of carrier materials with large surface area is beneficial for improving the adsorption affinity sites and enhancing the adsorption capacity. At present, various shapes of materials for use as adsorbents, such as spherical, sheet, rod, etc., are used in the field of hemoglobin separation and purification [23][24][25]. In these materials, urchin-like microspheres used as adsorbents have more binding sites to target proteins than spherical materials.…”

Section: Introductionmentioning

confidence: 99%

Cu(II)-Loaded Polydopamine-Coated Urchin-like Titanate Microspheres as a High-Performance IMAC Adsorbent for Hemoglobin Separation

Zhang,

Hu,

Yang

et al. 2024

Molecules

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Immobilized metal ion affinity chromatography (IMAC) adsorbents generally have excellent affinity for histidine-rich proteins. However, the leaching of metal ions from the adsorbent usually affects its adsorption performance, which greatly affects the reusable performance of the adsorbent, resulting in many limitations in practical applications. Herein, a novel IMAC adsorbent, i.e., Cu(II)-loaded polydopamine-coated urchin-like titanate microspheres (Cu-PDA-UTMS), was prepared via metal coordination to make Cu ions uniformly decorate polydopamine-coated titanate microspheres. The as-synthesized microspheres exhibit an urchin-like structure, providing more binding sites for hemoglobin. Cu-PDA-UTMS exhibit favorable selectivity for hemoglobin adsorption and have a desirable adsorption capacity towards hemoglobin up to 2704.6 mg g−1. Using 0.1% CTAB as eluent, the adsorbed hemoglobin was easily eluted with a recovery rate of 86.8%. In addition, Cu-PDA-UTMS shows good reusability up to six cycles. In the end, the adsorption properties by Cu-PDA-UTMS towards hemoglobin from human blood samples were analyzed by SDS-PAGE. The results showed that Cu-PDA-UTMS are a high-performance IMAC adsorbent for hemoglobin separation, which provides a new method for the effective separation and purification of hemoglobin from complex biological samples.

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One-step synthesis of superparamagnetic copper alginate gel microspheres (Cu2+-Alg@Fe3O4) using a compression nebulizer

Yang,

Guan,

Ren

et al. 2024

Chemical Engineering and Processing - Process Intensification

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Copper ion based metal–organic framework nanomaterials with roughness enhanced protein adhesion for high-efficiency hemoglobin separation

Abstract: Development of well-designed adsorption materials for the effective and specific purification of hemoglobin (Hb) from complex samples for disease diagnosis is still highly required. Herein, inspired by the concept of...

Cited by 9 publications

References 49 publications

Sustainable and Well-Dispersed Cu-Doped Magnetic Vortex Nanorings for Highly Specific Separation of His-Rich Proteins

Sustainable and Well-Dispersed Cu-Doped Magnetic Vortex Nanorings for Highly Specific Separation of His-Rich Proteins

Cu(II)-Loaded Polydopamine-Coated Urchin-like Titanate Microspheres as a High-Performance IMAC Adsorbent for Hemoglobin Separation

One-step synthesis of superparamagnetic copper alginate gel microspheres (Cu2+-Alg@Fe3O4) using a compression nebulizer

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