Tailoring head–tail mesoporous silica nanoparticles for enhanced gene transfection

Wu, Weixi; Ngô, Anh Dung; Ban, Wenhuang; Zhong, Yuenning; Cheng, Dan; Gu, Zhengying; Yu, Chengzhong; Song, Hao

doi:10.1039/d2tb01737g

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…In contrast, non-viral vectors have attracted widespread attention due to high safety, nonimmunogenicity, non-infectious, unlimited vector capacity, large-scale preparation, low cost, and targeting, which are also an indispensable class of efficient carriers in the field of gene therapy [13][14][15][16]. With the rapid development of nanotechnology, various organic and inorganic nanomaterials are used as carriers for drug and gene delivery, such as chitosan nanoparticles (NPs) [17][18][19], lipid-based NPs [20,21], polymer NPs [22][23][24], gold NPs [25,26], magnetic NPs [27], quantum dots (QDs) [28,29], silica NPs [30,31], and carbon nanomaterials [32,33] (Table 1). Among these non-viral vectors, iron-based nanomaterials possess both nanometer effects and superparamagnetism.…”

Section: Introductionmentioning

confidence: 99%

Recent progress of iron-based nanomaterials in gene delivery and tumor gene therapy

Gong,

Hu,

Chen

et al. 2024

J Nanobiotechnol

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Gene therapy aims to modify or manipulate gene expression and change the biological characteristics of living cells to achieve the purpose of treating diseases. The safe, efficient, and stable expression of exogenous genes in cells is crucial for the success of gene therapy, which is closely related to the vectors used in gene therapy. Currently, gene therapy vectors are mainly divided into two categories: viral vectors and non-viral vectors. Viral vectors are widely used due to the advantages of persistent and stable expression, high transfection efficiency, but they also have certain issues such as infectivity, high immunological rejection, randomness of insertion mutation, carcinogenicity, and limited vector capacity. Non-viral vectors have the advantages of non-infectivity, controllable chemical structure, and unlimited vector capacity, but the transfection efficiency is low. With the rapid development of nanotechnology, the unique physicochemical properties of nanomaterials have attracted increasing attention in the field of drug and gene delivery. Among many nanomaterials, iron-based nanomaterials have attracted much attention due to their superior physicochemical properties, such as Fenton reaction, magnetic resonance imaging, magnetothermal therapy, photothermal therapy, gene delivery, magnetically-assisted drug delivery, cell and tissue targeting, and so on. In this paper, the research progress of iron-based nanomaterials in gene delivery and tumor gene therapy is reviewed, and the future application direction of iron-based nanomaterials is further prospected.

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Section: Introductionmentioning

confidence: 99%

Recent progress of iron-based nanomaterials in gene delivery and tumor gene therapy

Gong,

Hu,

Chen

et al. 2024

J Nanobiotechnol

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Ion Doped Hollow Silica Nanoparticles as Promising Oligonucleotide Delivery Systems to Mesenchymal Stem Cells

Trayford,

Ibrahim,

van Rijt

2024

IJN

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Co-regulation of Sox9 and TGFβ1 transcription factors in mesenchymal stem cells regenerated the intervertebral disc degeneration

et al. 2023

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BackgroundIntervertebral disc (IVD) shows aging and degenerative changes earlier than any other body connective tissue. Its repair and regeneration provide a considerable challenge in regenerative medicine due to its high degree of infrastructure and mechanical complexity. Mesenchymal stem cells, due to their tissue resurfacing potential, represent many explanatory pathways to regenerate a tissue breakdown.MethodsThis study was undertaken to evaluate the co-regulation of Sox9 and TGFβ1 in differentiating human umbilical cord mesenchymal stem cells (hUC-MSC) into chondrocytes. The combinatorial impact of Sox9 and TGFβ1 on hUC-MSCs was examined in vitro by gene expression and immunocytochemical staining. In in vivo, an animal model of IVD degeneration was established under a fluoroscopic guided system through needle puncture of the caudal disc. Normal and transfected MSCs were transplanted. Oxidative stress, pain, and inflammatory markers were evaluated by qPCR. Disc height index (DHI), water content, and gag content were analyzed. Histological examinations were performed to evaluate the degree of regeneration.ResultshUC-MSC transfected with Sox9+TGFβ1 showed a noticeable morphological appearance of a chondrocyte, and highly expressed chondrogenic markers (aggrecan, Sox9, TGFβ1, TGFβ2, and type II collagens) after transfection. Histological observation demonstrated that cartilage regeneration, extracellular matrix synthesis, and collagen remodeling were significant upon staining with H&E, Alcian blue, and Masson's trichrome stain on day 14. Additionally, oxidative stress, pain, and inflammatory markers were positively downregulated in the animals transplanted with Sox9 and TGFβ1 transfected MSCs.ConclusionThese findings indicate that the combinatorial effect of Sox9 and TGFβ1 substantially accelerates the chondrogenesis in hUC-MSCs. Cartilage regeneration and matrix synthesis were significantly enhanced. Therefore, a synergistic effect of Sox9 and TGFβ1 could be an immense therapeutic combination in the tissue engineering of cartilaginous joint bio-prostheses and a novel candidate for cartilage stabilization.

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Tailoring head–tail mesoporous silica nanoparticles for enhanced gene transfection

Abstract: Plasmid DNA (pDNA) delivery has attracted extensive research interest due to its great potential in gene therapy. The design of efficient nano-vectors to promote cellular delivery and transfection of gene...

Cited by 3 publications

References 36 publications

Recent progress of iron-based nanomaterials in gene delivery and tumor gene therapy

Recent progress of iron-based nanomaterials in gene delivery and tumor gene therapy

Ion Doped Hollow Silica Nanoparticles as Promising Oligonucleotide Delivery Systems to Mesenchymal Stem Cells

Co-regulation of Sox9 and TGFβ1 transcription factors in mesenchymal stem cells regenerated the intervertebral disc degeneration

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