Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties

Martinez, Adela Helvia; Blanco,; Davidenko, Natalia; Cameron, Ruth E.

doi:10.1016/j.carbpol.2015.06.084

Cited by 121 publications

(72 citation statements)

References 51 publications

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“…We confirmed that the prosurvival effect of the QHREDGS peptide was preserved after it was conjugated and immobilized in the chitosan-collagen films and that the immobilized QHREDGS peptide was able to promote keratinocyte collective migration, whereas the soluble peptide did not. Notably, in the context of the chitosan-collagen film system, the conjugated QHREDGS peptide is presented in close proximity to collagen, thereby creating an environment reminiscent of the collagenglycosaminoglycan interaction in the native ECM (69).…”

Section: Discussionmentioning

confidence: 99%

Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization

Xiao

Reis

Feric

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

124

156

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There is a clinical need for new, more effective treatments for chronic wounds in diabetic patients. Lack of epithelial cell migration is a hallmark of nonhealing wounds, and diabetes often involves endothelial dysfunction. Therefore, targeting re-epithelialization, which mainly involves keratinocytes, may improve therapeutic outcomes of current treatments. In this study, we present an integrin-binding prosurvival peptide derived from angiopoietin-1, QHREDGS (glutamine-histidine-arginine-glutamic acid-aspartic acid-glycine-serine), as a therapeutic candidate for diabetic wound treatments by demonstrating its efficacy in promoting the attachment, survival, and collective migration of human primary keratinocytes and the activation of protein kinase B Akt and MAPK p42/44 . The QHREDGS peptide, both as a soluble supplement and when immobilized in a substrate, protected keratinocytes against hydrogen peroxide stress in a dose-dependent manner. Collective migration of both normal and diabetic human keratinocytes was promoted on chitosan-collagen films with the immobilized QHREDGS peptide. The clinical relevance was demonstrated further by assessing the chitosan-collagen hydrogel with immobilized QHREDGS in full-thickness excisional wounds in a db/db diabetic mouse model; QHREDGS showed significantly accelerated and enhanced wound closure compared with a clinically approved collagen wound dressing, peptide-free hydrogel, or blank wound controls. The accelerated wound closure resulted primarily from faster re-epithelialization and increased formation of granulation tissue. There were no observable differences in blood vessel density or size within the wound; however, the total number of blood vessels was greater in the peptide-hydrogeltreated wounds. Together, these findings indicate that QHREDGS is a promising candidate for wound-healing interventions that enhance reepithelialization and the formation of granulation tissue.

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Section: Discussionmentioning

confidence: 99%

Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization

Xiao

Reis

Feric

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

124

156

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“…The samples were mixed with 0.5 M acetic acid solution to a final concentration of 0.05% (w/v) and stirred at 4 °C until they were completely dissolved. The ζ-potential of ASC and PSC solutions at different pH values (2,3,4,5,6) were recorded.…”

Section: ζ -Potential Analysismentioning

confidence: 99%

Fish Skin Isolated Collagen Cryogels for Tissue Engineering Applications: Purification, Synthesis and Characterization

Bölgen¹,

Çetinkaya²,

Demir³

2016

J. Turk. Chem. Soc., Sect. A: Chem.

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Abstract:Tissue engineering aims regenerating damaged tissues by using porous scaffolds, cells, and bioactive agents. The scaffolds are produced from a variety of natural and synthetic polymers. Collagen is a natural polymer widely used for scaffold production because of its being the most important component of the connective tissue and biocompatibility. Cryogelation is a relatively simple technique compared to other scaffold production methods, which enables to produce interconnected porous matrices from the frozen reaction mixtures of polymers or monomeric precursors. Considering these, in this study, collagen was isolated from fish skin which is a non-commercial waste material, and scaffolds were produced from this collagen by cryogelation method. Pore morphology and protein structure of collagen was proven by SEM and UV-Vis analysis, respectively. Iso-electrical point of the protein was determined by zeta potential analysis. Amide A, Amide B, Amide I, Amide II and Amide III characteristic peaks of collagen was demonstrated by FTIR analysis. The yield of isolated protein was 14.53% and 2.42% for acid-soluble and pepsin-soluble collagen, respectively. Scaffolds were produced by crosslinking isolated acid soluble collagen with glutaraldehyde at cryogenic conditions. With FTIR analysis, C=N bond which belongs to the reaction between glutaraldehyde and collagen was found to be at 1655 cm -1 . It was demonstrated by SEM analysis that collagen and glutaraldeyhde concentration had significant effects on the pore morphology, diameter, and wall thickness of the cryogels, which in turn changed the swelling ratio and degradation profiles of the scaffolds. In this study, synthesis and characterization results of a fish skin isolated collagen cryogel scaffold that may be potentially used in the regeneration of damaged tissues are presented.

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“…12–17 As a further modifier of biological activity, collagen can also be combined with other polymers, such as chitosan or elastin, which influences chemical and mechanical properties. 18,19 …”

Section: Introductionmentioning

confidence: 99%

Collagen: a network for regenerative medicine

2016

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Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties

Cited by 121 publications

References 51 publications

Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization

Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization

Fish Skin Isolated Collagen Cryogels for Tissue Engineering Applications: Purification, Synthesis and Characterization

Collagen: a network for regenerative medicine

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