Tailored hydrogel composite membrane for the regulated crystallization of monosodium urate monohydrate within coffee’s metabolites system

Meng, Yingshuang; Qi, Zhibo; Li, Zhonghua; Niu, Yuchao; Wu, Mengyuan; Yuan, Zhijie; He, Gaohong; Yu, M. Y.; Jiang, Xiaobin

doi:10.1016/j.jcis.2023.05.183

Cited by 4 publications

(6 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To simulate the crystallization environment of MSUM in patients, a self-made bionic platform with a heterogeneous interface was constructed, and the bionic interface was prepared through a cross-linking reaction of polyethylene glycol diacrylate (PEGDA, cross-linker) and N -isopropylacrylamide (NIPAM, functional monomer) on polyvinylidene fluoride (PVDF) membrane. The preparation process and relevant characterizations of HCM were presented in detail in our previous work …”

Section: Resultsmentioning

confidence: 99%

“…The crystallization experiments were conducted in a self-made in vitro bionic platform, as presented in our previous work . One side of the membrane module was 100 mL of artificial body fluid and the other side was 100 mL of artificial joint fluid with 1.5 mM UA and 1 mL of 4.5 mg mL –1 HNPs (except for the control group).…”

Section: Methodsmentioning

confidence: 99%

“…The preparation process and relevant characterizations of HCM were presented in detail in our previous work. 26 Numerous studies have shown that the surface properties and structure of the modifiers have an important influence on their crystallization inhibition ability, especially charge, 8 hydrophobicity, 27 particle size, 28 and shape. 7 Despite great efforts that have been devoted to the search for MSUM crystallization inhibitors, they were almost random, extensive, and time-consuming due to an insufficient understanding of the inhibition mechanism.…”

Section: Synthesis Of Hcm and Hnps With High Electronegativitymentioning

confidence: 99%

“…The crystallization experiments were conducted in a self-made in vitro bionic platform, as presented in our previous work. 26 One side of the membrane module was 100 mL of artificial body fluid and the other side was 100 mL of artificial joint fluid with 1.5 mM UA and 1 mL of 4.5 mg mL −1 HNPs (except for the control group). The Na + concentration of artificial fluid was 140 mM, and the pH of the solutions was regulated to approximately 7.4 by 1 M NaOH or HCl aqueous solution.…”

Section: Synthesis Of Hnpsmentioning

confidence: 99%

See 3 more Smart Citations

Hydrogel Nanoparticles Enable Nucleation Barrier Regulation and Ion Anchoring as an Alternative Pathway for Monosodium Urate Monohydrate Crystallization Control

Meng,

Wang,

Zhao

et al. 2024

ACS Nano

Self Cite

View full text Add to dashboard Cite

Gout flare-up, commonly resulting from monosodium urate monohydrate (MSUM) crystallization, has led to painful inflammatory arthritis among hundreds of millions of people. Herein, a kind of hydrogel nanoparticles (HNPs) with specific properties was developed, aimed at providing a promising pathway for MSUM crystallization control. The experimental and molecular dynamics simulation results synchronously indicate that the fabricated HNPs achieve efficient inhibition of MSUM crystallization governed by the mechanism of “host–guest interaction” even under very low-dose administration. HNPs as the host dispersed in the hyperuricemic model effectively lift the relative heterogeneous nucleation barrier of the MSUM crystal and hinder solute aggregation with strong electronegativity and hydrophobicity. The initial appearance of MSUM crystals was then delayed from 94 to 334 h. HNPs as the guest on the surface of the formed crystal can decelerate the growth rate by anchoring ions and occupying the active sites on the surface, and the terminal yield of the MSUM crystal declined to less than 1% of the control group. The good biocompatibility of HNPs (cell viability > 94%) renders it possible for future clinical applications. This study can guide the rational design of inhibitory nanomaterials and the development of their application in the control of relevant pathological crystallization.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Synthesis Of Hcm and Hnps With High Electronegativitymentioning

confidence: 99%

Section: Synthesis Of Hnpsmentioning

confidence: 99%

See 2 more Smart Citations

Hydrogel Nanoparticles Enable Nucleation Barrier Regulation and Ion Anchoring as an Alternative Pathway for Monosodium Urate Monohydrate Crystallization Control

Meng,

Wang,

Zhao

et al. 2024

ACS Nano

Self Cite

View full text Add to dashboard Cite

show abstract

“…Gout arises from a high uric acid (UA) concentration due to abnormal purine metabolism, frequently manifested as acute gouty arthritis and hyperuricemia. − Facilitated by certain physiological conditions, elevated levels of UA precipitate via in vivo crystallization, forming sodium urate or other urate salt crystals . Among different types of urate crystals, sodium urate is the most prevalent in humans. , The deposition of these insoluble, acicular crystals within joints and tissues triggers the development of acute gouty arthritis. − Currently, pharmacological interventions available for gout management fail to efficiently degrade UA or impede the crystallization of sodium urate, and they often present adverse side effects. , Patients with severe symptoms may require surgical interventions, although such measures do not preclude recurrence …”

Section: Introductionmentioning

confidence: 99%

Adsorption of DNA and Aptamers to Sodium Urate Crystals and Inhibition of Crystal Growth

Yang,

Liu,

Wong

et al. 2024

Langmuir

View full text Add to dashboard Cite

An elevated level of blood uric acid (UA) can cause the formation of kidney stones, gout, and other diseases. We recently isolated a few DNA aptamers that can selectively bind to UA. In this work, we investigated the adsorption of a UA aptamer and random sequence DNA onto sodium urate crystals. Both DNA strands adsorbed similarly to urate crystals. In addition, both the UA aptamer and random DNA can inhibit the growth of urate crystals, suggesting a nonspecific adsorption mechanism rather than specific aptamer binding. In the presence of 500 nM DNA, the growth of needle-like sodium urate crystals was inhibited, and the crystals appeared granular after 6 h. To understand the mechanism of DNA adsorption, a few chemicals were added to desorb DNA. DNA bases contributed more to the adsorption than the phosphate backbone. Surfactants induced significant DNA desorption. Finally, DNA could also be adsorbed onto real UA kidney stones. This study provides essential insights into the interactions between DNA oligonucleotides and urate crystals, including the inhibition of growth and interface effects of DNA on sodium urate crystals.

show abstract

Modulation of the dissolution with ASP from a supersaturated solution on a bionic platform for gout pathology crystals

Liu,

Zhang,

Lei

et al. 2024

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Tailored hydrogel composite membrane for the regulated crystallization of monosodium urate monohydrate within coffee’s metabolites system

Cited by 4 publications

References 62 publications

Hydrogel Nanoparticles Enable Nucleation Barrier Regulation and Ion Anchoring as an Alternative Pathway for Monosodium Urate Monohydrate Crystallization Control

Hydrogel Nanoparticles Enable Nucleation Barrier Regulation and Ion Anchoring as an Alternative Pathway for Monosodium Urate Monohydrate Crystallization Control

Adsorption of DNA and Aptamers to Sodium Urate Crystals and Inhibition of Crystal Growth

Modulation of the dissolution with ASP from a supersaturated solution on a bionic platform for gout pathology crystals

Contact Info

Product

Resources

About