Tailorable Membrane‐Penetrating Nanoplatform for Highly Efficient Organelle‐Specific Localization

Zhang, Xin; Wang, Chunfei; Feng, Gang; Jiang, Junxiang; Hu, Jiwen; Rietz, Anna du; Brommesson, Caroline; Zhang, Xuanjun; Ma, Yan; Roberg, Karin; Zhang, Fengling; Shen, Han‐Ming; Hu, Zhangjun

doi:10.1002/smll.202101440

Cited by 4 publications

(2 citation statements)

References 55 publications

(60 reference statements)

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“…TPA‐based red emitting AIE‐TICT active NPs DTPA (Figure 10C) were fabricated with amphiphilic triblock copolymer pluorinic F127 composed of polypropylene oxide and polyethylene oxide. [ 156 ] F127 was used for encapsulation, which provides biocompatibility and stability to the AIE NPs. To make target specific for subcellular imaging of mitochondria and lysosome, these AIE NPs surface were further conjugated with TPP and morpholinyl (MP) chemoagent, respectively.…”

Section: Aie‐light Up Probes and Their Performancesmentioning

confidence: 99%

Review on recent trends and prospects in π‐conjugated luminescent aggregates for biomedical applications

et al. 2022

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Metal‐free organic molecules with structurally diverse aggregation‐induced emission (AIE) behavior and new functional photophysical properties reveal unique structure–property relationships, especially bright luminescence, and have attracted extensive attention in the past few decades. Despite tremendous progress on fluorescent molecules development, the extended π‐conjugated organic AIE probes with their nanorange self‐assembly, coassembly, unique morphology, high biocompatibility, and light‐harvesting capabilities enable them as potential candidates in numerous translational application perspectives. In particular, a few important classes of AIE light up small molecules, supramolecules, oligomers, polymers, including nanoparticles and photosensitizer molecules, with their emerging properties of thermally activated delayed fluorescence, room temperature phosphorescence, including emission switching stimuli‐responsive behavior and multifunctional properties, have been boosted by the rare features of aggregation at their condensed or solid states. This review highlights salient features of AIE‐based emitters, encompassing molecular design strategies, stimulating photophysical properties, mechanistic aspects, and their efficacy in various electronic and biomedical applications, broadly covering properties of small molecules to oligomers, macromolecules to polymers.

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Section: Aie‐light Up Probes and Their Performancesmentioning

confidence: 99%

Review on recent trends and prospects in π‐conjugated luminescent aggregates for biomedical applications

et al. 2022

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“…Under normal physiological conditions, mitochondria maintain an alkaline matrix (pH ∼ 8), reflecting proton extrusion across the inner membrane via the respiratory electron transport chain. , A breakdown in this normal function leads to reactive oxygen species (ROS) production, which acts as signaling molecules to initiate apoptosis . The protonmotive force further modulates dehydrogenase activity associated with the tricarboxylic acid (TCA) cycle, ATP synthase, and adenine nucleotide translocase by regulating intracellular Ca 2+ homeostasis. , Thus, understanding the rules and regulations of mitochondrial pH tethering to LDs provides new intervention points in relative biomedical metabolic diseases. − …”

mentioning

confidence: 99%

pH-Dominated Selective Imaging of Lipid Droplets and Mitochondria via a Polarity-Reversible Ratiometric Fluorescent Probe

Bai

Yang

et al. 2022

Anal. Chem.

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Elucidating the intrinsic relationship between mitochondrial pH (pH m ) fluctuation and lipid droplets (LDs) formation is vital in cell physiology. The development of small-molecular fluorescent probes for discrimination and simultaneous visualization of pH m fluctuation toward LDs has not yet been reported. In this work, utilizing pH-driven polarityreversible hemicyanine and rhodamine derivatives, a multifunctional fluorescent probe is developed for selectively identifying mitochondria and LDs under specific pH values via dual-emission channels. This rapidresponse probe, Hcy-Rh, has two distinct chemical structures under acidic and alkaline circumstances. In acidic conditions, Hcy-Rh exhibits good hydrophilicity that can target mitochondria and display an intense red fluorescence. Conversely, the probe becomes lipophilic under weakly alkaline conditions and targets LDs, showing a strong blue emission. In this manner, Hcy-Rh can selectively label mitochondria and LDs, exhibiting red and blue fluorescence, respectively. Moreover, this ratiometric probe is applied to map pH m changes in living cells under the stimulus with FCCP, NAC, and H 2 O 2 . The interplay of LD− mitochondria under oleic acid treatment and starvation-induced autophagy has been studied using this probe at different pH values. In a word, Hcy-Rh is a potential candidate for further exploring mitochondria−LD interaction mechanisms under pH m fluctuation. Moreover, the polarity-dependent strategy is valuable for designing other functional biological probes in imaging multiple organelles.

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Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy

Zhang

Chen

et al. 2022

Nano Res.

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Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria, thereby pH fluctuation is one of the most significant indicators for tracking mitophagy. Then such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features, including mitochondrial-specificity, excellent biocompatibility, wide pH-sensitive range of 8.0–4.0, and especially quantitative ability. However, available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single molecule. To fully address this issue, we herein integrate two fluorogenic pH sensitive units, a mitochondria-specific block, cell-penetrating facilitator, and biocompatible segments into an elegant silica nano scaffold, which greatly ensures the applicability for real-time tracking of pH fluctuations in mitophagy. Most significantly, at a single wavelength excitation, the integrated pH-sensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH, thus enabling a ratiometric analysis of pH variations during the whole mitophagy. This work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications.

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Tailorable Membrane‐Penetrating Nanoplatform for Highly Efficient Organelle‐Specific Localization

Cited by 4 publications

References 55 publications

Review on recent trends and prospects in π‐conjugated luminescent aggregates for biomedical applications

Review on recent trends and prospects in π‐conjugated luminescent aggregates for biomedical applications

pH-Dominated Selective Imaging of Lipid Droplets and Mitochondria via a Polarity-Reversible Ratiometric Fluorescent Probe

Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy

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