Tailor-made derivatizing agents for carbonyl compounds using liquid chromatography

Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

“…However, the derivatizing reagent and derivatives with short alkyl chains such as formaldehyde hydrazone, are rather difficult to separate with the mobile phase used for the homologous series, because the differences in the properties of these compounds are relatively small but the mobile phase is too strong to distinguish such small differences. The HPLC method [1,2] employed a binary step gradient elution: a step of weakelution strength (containing 38% v/v acetonitrile) for more polar compounds and another step of strong elution strength (containing 90% v/v acetonitrile) for more hydrophobic compounds. Because of the higher efficiency of CEC, it is possible to use isocratic CEC separation as an alternative to gradient HPLC separation [24,25].…”

“…4-Dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH), developed by Kempter et al [1,2], is a new derivatizing reagent specially designed for the determination of carbonyl compounds by HPLC. It is characterized by a detectable group (for UV-VIS and fluorescence detection), a reactive group and a third group to influence the polarity of the whole derivatizing reagent in its molecular structure [1].…”

“…It is characterized by a detectable group (for UV-VIS and fluorescence detection), a reactive group and a third group to influence the polarity of the whole derivatizing reagent in its molecular structure [1]. Table 1 shows the structures of the DMNTH and ten carbonyl DMNTH derivatives, which have been separated by HPLC.…”

“…The HPLC method has proved to have the advantages of easy sample preparation, low limits of detection without enrichment and excellent reproducibility [2]. Due to the large differences in the properties of these compounds, gradient elution was needed in the HPLC method [1,2].…”

Evaluation of fluorescent monochloro-s-triazine reagent for amino acid analysis

“…The remaining chlorine serves as a reactive functional group capable of reacting with analytes such as amino acids or a protein. There is little doubt that variations of this generally applicable strategy will lead to an abundance of reagents suitable for reacting with nucleophilic target compounds of interest [23,24].…”