Evaluation of fluorescent monochloro-s-triazine reagent for amino acid analysis

Abstract

SummaryThe fluorescent reagent 2-chloro-4-methoxy-6-(4-methoxy-1-na phthyl)-[1,3,5lt riazine (CMMNT) has been synthesized and used for derivatization of mixtures of 0~-amino acids. The derivatives were resolved on a Nucleosil | Cls column using a hnear gradient of 0.1% aqueous trifluoroacetic acid in acetonitrile and detected by their fluorescence at 415 nm using an excitation of 336 nm. Using a standard the linearity was determined from 0.13 -26 pmol per amino acid derivative and the detection limit was 6.6 x 10 -2 pmol at a signal-to-noise ratio of 3.5. This approach was used for quantitative, amino acid analysis of total hydrolysates of synthetic peptides and for fluorescence labelling of casein.

Enantioresolution of five β‐blockers by reversed‐phase high‐performance liquid chromatography using fifteen chiral derivatizing reagents having amino acids or their amides as chiral auxiliaries on a cyanuric chloride platform

“…Enantioseparation of α ‐amino acids has also been reported using two CC‐based CDRs that had piperidine as achiral auxiliary and l ‐Leu‐NH 2 and l ‐Leu as chiral auxiliaries (Bhushan and Agarwal, ). The literature reveals that CC‐based CDRs have also been used for chiral separation of racemic mixtures of amino acids using RP‐HPLC (Brückner and Strecker ; Brückner and Wachsmann 2003a, b) and micellar electrokinetic chromatography (Ma et al , ).…”