One-pot labeling-based capillary zone electrophoresis for separation of amino acid mixture and assay of biofluids

Song, Lijuan; Guo, Zhenpeng; Chen, Yi

doi:10.1016/j.aca.2011.07.042

Cited by 14 publications

(7 citation statements)

References 46 publications

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“…The other tested BGE was a borate buffer. Borate buffer is often used for separation of various compounds in biological fluids by CE with UV or LIF detection and we have used it successfully in selected CE–LIF analytical application . Borate buffer was tested at various concentrations ranging from 10 to 50 mM at pH 7.5.…”

Section: Resultsmentioning

confidence: 99%

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

et al. 2019

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In this article, optimization of BGE for simultaneous separation of inorganic ions, organic acids, and glutathione using dual C4D‐LIF detection in capillary electrophoresis is presented. The optimized BGE consisted of 30 mM 2‐[4‐(2‐hydroxyethyl)piperazin‐1‐yl]ethanesulfonic acid, 15 mM 2‐amino‐2‐hydroxymethyl‐propane‐1,3‐diol, and 2 mM 18‐crown‐6 at pH 7.2 and allowed simultaneous separation of ten inorganic anions and cations, three organic acids and glutathione in 20 min. The samples were injected hydrodynamically from both capillary ends using the double‐opposite end injection principle. Sensitive detection of anions, cations, and organic acids with micromolar LODs using C4D and simultaneously glutathione with nanomolar LODs using LIF was achieved in a single run. The developed BGE may be useful in analyses of biological samples containing analytes with differing concentrations of several orders of magnitude that is not possible with single detection mode.

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Section: Resultsmentioning

confidence: 99%

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

et al. 2019

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“…All the PenA‐correlative samples were derivatized by one‐pot technique according to reference []. In detail, ANDA (10.3 mg, 30 μmol) was dissolved in 300‐μL water together with solid NaOH (2.4 mg, 60 μmol).…”

Section: Methodsmentioning

confidence: 99%

“…The core idea is to make the analytes charged strongly to enable their electromigration in broad pH and bear ideal chromophore group(s) to facilitate the detection. This can in theory be achieved by simultaneously labeling the analytes with chromophore and strongly charged groups, and after a hard study, a facile and cost‐effective one‐pot labeling technique has developed in our laboratory to fast labeling amino acids []. In this paper, this one‐pot technique is combined into the chiral separation of dl ‐PenA and will be illustrated that it meets with the requirements what we expected.…”

Section: Introductionmentioning

confidence: 92%

Separation and determination of chiral composition in penicillamine tablets by capillary electrophoresis in a broad pH range

2012

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A chiral capillary electrophoretic method with nearly full pH window was explored for the separation and determination of dl-penicillamine. A facile one-pot labeling technique was coupled in the method for introduction of chromophore and charge groups onto the analytes to facilitate the electromigration and sensitive detection. By using simply a cost-effective neutral β-cyclodextrin as chiral selector, baseline separation of the dl-penicillamine was achieved from pH 2.0 to over pH 10. Quantification of standard d- and l-penicillamines was demonstrated by taking pH 4.5, 7.4, and 9.7 as the representatives of acidic, neutral, and basic conditions. The working curves were constructed between peak area and concentration, having linear ranges of 8.56-8.56 × 10(2) μg/mL for pH 4.5 and 8.56-1.71 × 10(3) μg/mL for pH 7.4 and 9.7, with correlation coefficients all better than 0.999. The limit of detection (S/N = 3) was 2.58 μg/mL in acidic and neutral conditions or 1.41 μg/mL in basic condition. The method was further validated by assaying the commercial penicillamine tablets, applicable to quantification of the effective enantiomer and the trace impurity of l-penicillamine at a content of down to 0.2, 0.6, and 2.0% for pH 9.7, 4.5, and 7.4, respectively. The recovery determined by spiking technique was in a range from 93.1 to 105 %. The method is easily extendable to the analysis of other chiral amines or amino acids.

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“…A fast, simple, and cost‐effective one‐pot labeling method has been developed , including two reaction steps of: cyanuric chloride was made to react first with 7‐amino‐1,3‐naphthalenedisulfonic acid monopotassium salt, and then with AAs. Using a running sodium tetraborate buffer, 19 AAs were efficiently separated in 25 min, with good reproducibility.…”

Section: Applicationsmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods, 2011–2013

et al. 2013

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This article describes the most important research published on amino acid (AA) analysis using CE during the period from June 2011 to May 2013, and follows the format of the previous articles of Smith (Electrophoresis 1999, 20, 3078-3083), Prata et al. (Electrophoresis 2001, 22, 4129-4138), and Poinsot et al. (Electrophoresis 2003, 24, 4047-4062; Electrophoresis 2006, 27, 176-194; Electrophoresis 2008, 29, 207-223; Electrophoresis 2010, 31, 105-121; Electrophoresis 2012, 33, 14-35). We present new developments in AA analysis with CE, mainly describing the use of MS or LEDs for detection following conventional or enantiomeric separation developments. In addition, in an application part, we describe neurochemical or clinical studies, metabolomics for plant extracts and biological fluids, and finally works focused on AAs in food and agricultural applications.

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One-pot labeling-based capillary zone electrophoresis for separation of amino acid mixture and assay of biofluids

Cited by 14 publications

References 46 publications

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

Optimization of background electrolyte composition for simultaneous contactless conductivity and fluorescence detection in capillary electrophoresis of biological samples

Separation and determination of chiral composition in penicillamine tablets by capillary electrophoresis in a broad pH range

Recent advances in amino acid analysis by capillary electromigration methods, 2011–2013

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