Taguchi Grey Relational Analysis for Multi-Response Optimization of Bacillus Bacteria Flocculation Recovery from Fermented Broth by Chitosan to Enhance Biocontrol Efficiency

Abstract

Degradation of environment is a challenge to crop production around the world. Biological control of various plant diseases using antagonistic bacteria is an encouraging alternative to traditionally used chemical control strategies. Chitosan as a well-known natural flocculation agent also exhibits antimicrobial activity. The goal of this study was to investigate a dual nature of chitosan in flocculation of Bacillus sp. BioSol021 cultivation broth intended for biocontrol applications. Experiments were performed based on L18 standard Taguchi orthogonal array design with five input parameters (chitosan type and dosage, pH value, rapid and slow mixing rates). In this study, the grey relational analysis was used to perform multi-objective optimization of the chosen responses, i.e., flocculation efficiency and four inhibition zone diameters against the selected phytopathogens. The results have indicated a great potential of a highly efficient method for removal of the Bacillus bacteria from the cultivation broth using chitosan. The good flocculation efficiency and high precipitate antimicrobial activity against the selected phytopathogens were achieved. It has been shown that multiple flocculation performance parameters were improved, resulting in slightly improved response values.

Dairy and Wine Industry Effluents as Alternative Media for the Production of Bacillus-Based Biocontrol Agents

“…The producing microorganism used in this study was Bacillus sp. strain BioSol021, isolated from the rhizosphere of common beans or kidney beans ( Phaseolus vulgaris ) and identified using 16S rRNA gene sequencing (GenBank sequence accession number ON569805) and biochemical characterization using VITEK2 BCL cards (Biomerieux, Marcy-l′Étoile, France) as a member of the operational group Bacillus amyloliquefaciens [ 39 ]. The test microorganisms or phytopathogens used in this study were Aspergillus flavus strains SA2B SS and PA2D SS isolated from maize with proven aflatoxigenic potential [ 40 ].…”

“…In each well, 100 μL of the cultivation broth sample was added. The incubation was performed at 26 °C for 96 h, followed by measurements of the inhibition zone diameters [ 39 , 40 ].…”

The Effect of Cultivation Conditions on Antifungal and Maize Seed Germination Activity of Bacillus-Based Biocontrol Agent

“…BioSol021, was previously isolated from the rhizosphere of Phaseolus vulgaris and selected among 76 Bacillus strains as the most efficient biocontrol agent in A. flavus suppression [ 2 ]. The 16S rRNA sequencing and VITEK2 Compact System identification indicated the highest similarity of the producing microorganism to the members of the operational group Bacillus amyloliquefaciens (GenBank sequence accession number ON569805) [ 29 ]. The producing strain is deposited at the international depository service under the Budapest Treaty in the International Collection of Agricultural and Industrial Microorganisms, Faculty of Food Science, Szent István University, Budapest, Hungary, with the collection number 520/2021.…”

Valorization of barrel washing winery wastewater through production of microbial biocontrol agents

“…BioSol021 isolated from the rhizosphere of common bean (Phaseolus vulgaris) was used in this study as a biocontrol strain, with previous record of biocontrol activity (13). The producing strain was identified using 16S rRNA gene sequencing (GenBank sequence accession number ON569805) and biochemically characterized using VITEK2 Compact System (14), where the results have shown the highest similarity to the operational group Bacillus amyloliquefaciens. The phytopathogenic bacteria used as test microorganisms in this study were Xanthomonas campestris Mn 7-2, isolated from cabbage with black rot symptoms, and Xanthomonas euvesicatoria PL1, isolated from pepper leaves with (15).…”