Tagging (Arene)ruthenium(II) Anticancer Complexes with Fluorescent Labels

Abstract

Fluorescent (arene)ruthenium(II) complexes have been prepared by tagging a small fluorogenic reporter onto the chelating ligand of complexes of the type [(η 6 -arene)RuCl(Z)]

Synthesis of Pincer Ruthenium RuCl(CNN)(PP) Catalysts from [RuCl(μ-Cl)(η6-p-cymene)]2

“…The 1 H NMR spectrum of 1a at 50 °C shows four doublets for the aromatic p -cymene protons with two doublets for the isopropyl moiety, whereas the two multiplets at δ = 4.50 are for the two diastereotopic NCH 2 protons. These data are consistent with a cationic complex with a chloride as counterion, as observed for analogous ruthenium complexes . While one N–H proton is at δ = 3.28 and appears as a broad quartet, the second N–H is significantly low-field shifted at δ = 9.46 (broad triplet), as established through a 1 H– 1 H COSY experiment, and it is consistent with an interaction of the N–H proton with the outer sphere chloride.…”

In Vivo Selectivity and Localization of Reactive Oxygen Species (ROS) Induction by Osmium Anticancer Complexes That Circumvent Platinum Resistance

“…Optimal absorption and emission wavelengths for fluorescence studies were determined (ex/em = 560/580 nm, Figure b). Perhaps surprisingly, fluorescence was not quenched by Os in the complex, unlike in several previous reports of labeling transition metal complexes …”

“…Perhaps surprisingly, fluorescence was not quenched by Os in the complex, unlike in several previous reports of labeling transition metal complexes. 45 …”

“…Perhaps surprisingly, fluorescence was not quenched by Os in the complex, unlike in several previous reports of labeling transition metal complexes. 45 Next, A2780 cancer cells were treated with red-fluorescent complex 6 (Figure 3a) and then stained with the green ROSdetection reagent (Enzo Life Sciences, Figure 3c) previously used in our flow cytometry investigations (Figure 2). The two emission bands were readily resolved, and clear colocalization (yellow) was observed between green (ROS production) and red (complex 6) fluorescence.…”

Monitoring changes of paramagnetically-shifted 31P signals in phospholipid vesicles

“…Tris is well known to act as a chelating agent, while HEPES was originally described as a non-chelator [33], more recently it has been shown to bind "soft" metals such as silver (I) [34], mercury (II) [35] and ruthenium (II) [36]. To test whether the buffers were interacting with the praseodymium (III), POPC vesicles were prepared in sodium chloride solution only, [18,37,38], however, investigation of the role of the buffer appears to be neglected.…”