TaEXPB7-B, a β-expansin gene involved in low-temperature stress and abscisic acid responses, promotes growth and cold resistance in Arabidopsis thaliana

Xu, Feng; Xu, Yongqing; Peng, Lina; Yu, Xingyu; Zhao, Qing Shan; Feng, Shanshan; Zhao, Ziyi; Li, Fenglan; Bao-zhong, HU

doi:10.1016/j.jplph.2019.153004

Cited by 42 publications

(27 citation statements)

References 68 publications

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“…Expansins are plant cell wall loosening proteins that have been implicated in the control of plant growth via the pH-dependent extension of plant cell walls [57]. Expansins have also been known to be involved in abiotic stress response by cell wall loosening in various species, such as maize [58], soybean [59], Arabidopsis [34], and wheat [35]. The involvement of some expansin family genes in the abiotic stress response and plant development has been reported in rice.…”

Section: Osexpa7 Overexpression Improved Salt Stress Tolerance In Ricementioning

confidence: 99%

“…GmEXPLB1 in soybeans [32] respond to waterlogging. IbEXP1, IbEXP2, and IbEXPL1 genes are located in sweet-potato [33], AtEXLA2 in are found in Arabidopsis [34], and TaEXPB7-B is located in wheat [35], which responds to heat. Additionally, the AsEXP1 gene is present in turf grass [36], and TaEXPA2 [37] and ZmEXPB6 is located in maize [38], which respond to salt tolerance.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba

Kang

Paek

et al. 2020

IJMS

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Expansins are key regulators of cell-wall extension and are also involved in the abiotic stress response. In this study, we evaluated the function of OsEXPA7 involved in salt stress tolerance. Phenotypic analysis showed that OsEXPA7 overexpression remarkably enhanced tolerance to salt stress. OsEXPA7 was highly expressed in the shoot apical meristem, root, and the leaf sheath. Promoter activity of OsEXPA7:GUS was mainly observed in vascular tissues of roots and leaves. Morphological analysis revealed structural alterations in the root and leaf vasculature of OsEXPA7 overexpressing (OX) lines. OsEXPA7 overexpression resulted in decreased sodium ion (Na+) and accumulated potassium ion (K+) in the leaves and roots. Under salt stress, higher antioxidant activity was also observed in the OsEXPA7-OX lines, as indicated by lower reactive oxygen species (ROS) accumulation and increased antioxidant activity, when compared with the wild-type (WT) plants. In addition, transcriptional analysis using RNA-seq and RT-PCR revealed that genes involved in cation exchange, auxin signaling, cell-wall modification, and transcription were differentially expressed between the OX and WT lines. Notably, salt overly sensitive 1, which is a sodium transporter, was highly upregulated in the OX lines. These results suggest that OsEXPA7 plays an important role in increasing salt stress tolerance by coordinating sodium transport, ROS scavenging, and cell-wall loosening.

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Section: Osexpa7 Overexpression Improved Salt Stress Tolerance In Ricementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba

Kang

Paek

et al. 2020

IJMS

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“…However, the specific physiological role of each expansin under normal or stressful conditions is still unknown. In this regard, it has been demonstrated that overexpression of TaEXPB7‐B in Arabidopsis enhanced plant tolerance to low‐temperature stress (Feng et al, 2019). Furthermore, the ectopic expression of TaEXPB23 was associated with phosphorus availability (Han et al, 2014) and drought (Li, Xing, et al, 2011b), as well as in oxidative stress tolerance (Han et al, 2015; Li et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress

Mansur

Pena

Bossio³

et al. 2021

Physiologia Plantarum

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Cadmium is one of the most important contaminants and it induces severe plant growth restriction. In this study, we analyzed the metabolic changes associated with root growth restriction caused by cadmium in the early seminal root apex of wheat. Our study included two genotypes: the commercial variety ProINTA Federal (WT) and the PSARK::IPT (IPT) line which exhibit high‐grade yield performance under water deficit. Root tips of seedlings grown for 72 h without or with 10 μM CdCl2 (Cd‐WT and Cd‐IPT) were compared. Root length reduction was more severe in Cd‐WT than Cd‐IPT. Cd decreased superoxide dismutase activity in both lines and increased catalase activity only in the WT. In Cd‐IPT, ascorbate and guaiacol peroxidase activities raised compared to Cd‐WT. The hormonal homeostasis was altered by the metal, with significant decreases in abscisic acid, jasmonic acid, 12‐oxophytodienoic acid, gibberellins GA20, and GA7 levels. Increases in flavonoids and phenylamides were also found. Root growth impairment was not associated with a decrease in expansin (EXP) transcripts. On the contrary, TaEXPB8 expression increased in the WT treated by Cd. Our findings suggest that the line expressing the PSARK::IPT construction increased the homeostatic range to cope with Cd stress, which is visible by a lesser reduction of the root elongation compared to WT plants. The decline of root growth produced by Cd was associated with hormonal imbalance at the root apex level. We hypothesize that activation of phenolic secondary metabolism could enhance antioxidant defenses and contribute to cell wall reinforcement to deal with Cd toxicity.

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“…The results ( Fig. 5 ) showed that MYB, MBS, TC-rich, LTR, GT1-motif, G-Box, GA-motif, W-Box, ABRE, ERE, and GARE-motifs, which were related to drought tolerance ( Zhang et al, 2019 ), flavonoid biosynthesis ( Wang et al, 2018 ), defense and stress responsiveness ( Zhang et al, 2005 ), low temperature tolerance ( Feng et al, 2019 ), light response ( Zhang et al, 2013 ; Zhu et al, 2015 ), abiotic stress responsiveness ( Yan et al, 2019 ), abscisic acid responsiveness ( Choi et al, 2005 ), ethylene responsiveness ( Rawat et al, 2005 ), and gibberellin responsiveness, were found in promoters of the 26 BpDof genes. Among these, MYB, MBS, G-Box, GT1-motif, ABRE, and ERE cis-elements were abundant, thus suggesting that these BpDof genes might be involved in drought tolerance, light response, and ABA- and ethylene-responsive signaling.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of BpDof genes and the tolerance to drought stress in birch (Betula platyphylla)

Sun

Wang

Jiang

et al. 2021

PeerJ

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Background DNA binding with one finger (Dof) proteins are plant-specific transcription factors playing vital roles in developmental processes and stress responses in plants. Nevertheless, the characterizations, expression patterns, and functions of the Dof family under drought stress (a key determinant of plant physiology and metabolic homeostasis) in woody plants remain unclear. Methods The birch (Betula platyphylla var. mandshuric) genome and plant TFDB database were used to identify Dof gene family members in birch plants. ClustalW2 of BioEdit v7.2.1, MEGA v7.0, ExPASy ProtParam tool, Subloc, TMHMM v2.0, GSDS v2.0, MEME, TBtools, KaKs Calculator v2.0, and PlantCARE were respectively used to align the BpDof sequences, build a phylogenetic tree, identify the physicochemical properties, analyze the chromosomal distribution and synteny, and identify the cis-elements in the promoter regions of the 26 BpDof genes. Additionally, the birch seedlings were exposed to PEG6000-simulated drought stress, and the expression patterns of the BpDof genes in different tissues were analyzed by qRT-PCR. The histochemical staining and the evaluation of physiological indexes were performed to assess the plant tolerance to drought with transient overexpression of BpDof4, BpDof11, and BpDof17 genes. SPSS software and ANOVA were used to conduct all statistical analyses and determine statistically significant differences between results. Results A total of 26 BpDof genes were identified in birch via whole-genome analysis. The conserved Dof domain with a C(x)2C(x)21C(x)2C zinc finger motif was present in all BpDof proteins. These birch BpDofs were classified into four groups (A to D) according to the phylogenetic analysis of Arabidopsis thaliana Dof genes. BpDof proteins within the same group mostly possessed similar motifs, as detected by conserved motif analysis. The exon–intron analysis revealed that the structures of BpDof genes differed, indicating probable gene gain and lose during the BpDof evolution. The chromosomal distribution and synteny analysis showed that the 26 BpDofs were unevenly distributed on 14 chromosomes, and seven duplication events among six chromosomes were found. Cis-acting elements were abundant in the promoter regions of the 26 BpDof genes. qRT-PCR revealed that the expression of the 26 BpDof genes was differentially regulated by drought stress among roots, stems, and leaves. Most BpDof genes responded to drought stress, and BpDof4, BpDof11, and BpDof17 were significantly up-regulated. Therefore, plants overexpressing these three genes were generated to investigate drought stress tolerance. The BpDof4-, BpDof11-, and BpDof17-overexpressing plants showed promoted reactive oxygen species (ROS) scavenging capabilities and less severe cell damage, suggesting that they conferred enhanced drought tolerance in birch. This study provided an in-depth insight into the structure, evolution, expression, and function of the Dof gene family in plants.

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TaEXPB7-B, a β-expansin gene involved in low-temperature stress and abscisic acid responses, promotes growth and cold resistance in Arabidopsis thaliana

Cited by 42 publications

References 68 publications

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

An isopentenyl transferase transgenic wheat isoline exhibits less seminal root growth impairment and a differential metabolite profile under Cd stress

Genome-wide analysis of BpDof genes and the tolerance to drought stress in birch (Betula platyphylla)

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