Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba, Chuluuntsetseg; Kang, Kiyoon; Paek, Nam‐Chon; Lee, Soo In; Yoo, Soo-Cheul

doi:10.3390/ijms21020454

Cited by 71 publications

(56 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the overaccumulation of expansins under salt stress would not favor cell wall expansion but rather participate to the salt stress signaling pathway. Several studies showed that overexpression of an expansin gene in Arabidopsis was shown to confer enhanced salt tolerance by lowering ROS accumulation and increasing antioxidant activity ( Abuqamar et al, 2013 ; Lü et al, 2013 ; Chen et al, 2019 ; Jadamba et al, 2020 ). Thus, the higher abundance of FLAs associated with a higher overaccumulation of expansins observed in Ws could contribute to a better CWI in response to salt stress than Col-0, where only one FLA and WAK1 were overaccumulated.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

et al. 2021

View full text Add to dashboard Cite

Salinity affects plant growth and development as shown with the glycophyte model plant, Arabidopsis thaliana (Arabidopsis). Two Arabidopsis accessions, Wassilewskija (Ws) and Columbia (Col-0), are widely used to generate mutants available from various Arabidopsis seed resources. However, these two ecotypes are known to be salt-sensitive with different degrees of tolerance. In our study, 3-week-old Col-0 and Ws plants were treated with and without 150 mM NaCl for 48, 72, or 96 h, and several physiological and biochemical traits were characterized on shoots to identify any specific traits in their tolerance to salinity. Before salt treatment was carried out, a different phenotype was observed between Col-0 and Ws, whose main inflorescence stem became elongated in contrast to Col-0, which only displayed rosette leaves. Our results showed that Col-0 and Ws were both affected by salt stress with limited growth associated with a reduction in nutrient uptake, a degradation of photosynthetic pigments, an increase in protein degradation, as well as showing changes in carbohydrate metabolism and cell wall composition. These traits were often more pronounced in Col-0 and occurred usually earlier than in Ws. Tandem Mass Tags quantitative proteomics data correlated well with the physiological and biochemical results. The Col-0 response to salt stress was specifically characterized by a greater accumulation of osmoprotectants such as anthocyanin, galactinol, and raffinose; a lower reactive oxygen detoxification capacity; and a transient reduction in galacturonic acid content. Pectin degradation was associated with an overaccumulation of the wall-associated kinase 1, WAK1, which plays a role in cell wall integrity (CWI) upon salt stress exposure. Under control conditions, Ws produced more antioxidant enzymes than Col-0. Fewer specific changes occurred in Ws in response to salt stress apart from a higher number of different fascilin-like arabinogalactan proteins and a greater abundance of expansin-like proteins, which could participate in CWI. Altogether, these data indicate that Col-0 and Ws trigger similar mechanisms to cope with salt stress, and specific changes are more likely related to the developmental stage than to their respective genetic background.

show abstract

Section: Discussionmentioning

confidence: 99%

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Down-regulation of ZmEXPB6 is correlated with the reduced leaf growth of maize under salt stress [ 89 ]. Overexpression of rice expansin 7 ( OsEXPA7 ) confers substantially enhanced tolerance to salt stress by lowering reactive oxygen species (ROS) accumulation and increasing antioxidant activity in rice [ 90 ]. Ectopic expression of wheat expansin 2 ( TaEXPA2 ) or TaEXPB23 improves salt tolerance in tobacco [ 91 , 92 ].…”

Section: The Roles Of the Cell Wall-localized Glycoproteins In Salmentioning

confidence: 99%

Maintenance of Cell Wall Integrity under High Salinity

Liu

Zhang

Long

et al. 2021

IJMS

View full text Add to dashboard Cite

Cell wall biosynthesis is a complex biological process in plants. In the rapidly growing cells or in the plants that encounter a variety of environmental stresses, the compositions and the structure of cell wall can be dynamically changed. To constantly monitor cell wall status, plants have evolved cell wall integrity (CWI) maintenance system, which allows rapid cell growth and improved adaptation of plants to adverse environmental conditions without the perturbation of cell wall organization. Salt stress is one of the abiotic stresses that can severely disrupt CWI, and studies have shown that the ability of plants to sense and maintain CWI is important for salt tolerance. In this review, we highlight the roles of CWI in salt tolerance and the mechanisms underlying the maintenance of CWI under salt stress. The unsolved questions regarding the association between the CWI and salt tolerance are discussed.

show abstract

“…Thirty-six and 38 genes were homologous to arabidopsis and rice, respectively (Supplementary Table 7). Six predicted genes, included AET2Gv20774800, AET4Gv20799000, AET5Gv20005900, AET5Gv20084100, AET7Gv20644900, and AET5Gv21111700, were homologous to MST1 (Takeda et al, 2001;Mao et al, 2011), MAC3B (Monaghan et al, 2009;Li S. et al, 2018), ETR1 (Yin et al, 2017), ZAR1 (Guo et al, 2013;Yu et al, 2016), NAC047 (Kunieda et al, 2008;Mathew et al, 2016), EXPA7 (Lizana et al, 2010;Jadamba et al, 2020), respectively (Table 5). These genes (MST1, MAC3B, ETR1, ZAR1, NAC047, EXPA7) could affect embryo development, cause seed surface atrophy, increase the number of cells to increase organ size, or affect kernel size through ethylene response.…”

Section: Candidate Genes That May Be Linked To Kernel Traitsmentioning

confidence: 99%

Genome-Wide Association Study of Kernel Traits in Aegilops tauschii

et al. 2021

View full text Add to dashboard Cite

Aegilops tauschii is the diploid progenitor of the D subgenome of hexaploid wheat (Triticum aestivum L.). Here, the phenotypic data of kernel length (KL), kernel width (KW), kernel volume (KV), kernel surface area (KSA), kernel width to length ratio (KWL), and hundred-kernel weight (HKW) for 223 A. tauschii accessions were gathered across three continuous years. Based on population structure analysis, 223 A. tauschii were divided into two subpopulations, namely T-group (mainly included A. tauschii ssp. tauschii accessions) and S-group (mainly included A. tauschii ssp. strangulata). Classifications based on cluster analysis were highly consistent with the population structure results. Meanwhile, the extent of linkage disequilibrium decay distance (r2 = 0.5) was about 110 kb and 290 kb for T-group and S-group, respectively. Furthermore, a genome-wide association analysis was performed on these kernel traits using 6,723 single nucleotide polymorphism (SNP) markers. Sixty-six significant markers, distributed on all seven chromosomes, were identified using a mixed linear model explaining 4.82–13.36% of the phenotypic variations. Among them, 15, 28, 22, 14, 21, and 13 SNPs were identified for KL, KW, KV, KSA, KWL, and HKW, respectively. Moreover, six candidate genes that may control kernel traits were identified (AET2Gv20774800, AET4Gv20799000, AET5Gv20005900, AET5Gv20084100, AET7Gv20644900, and AET5Gv21111700). The transfer of beneficial genes from A. tauschii to wheat using marker-assisted selection will broaden the wheat D subgenome improve the efficiency of breeding.

show abstract

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Cited by 71 publications

References 109 publications

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

Physiological and Biochemical Traits of Two Major Arabidopsis Accessions, Col-0 and Ws, Under Salinity

Maintenance of Cell Wall Integrity under High Salinity

Genome-Wide Association Study of Kernel Traits in Aegilops tauschii

Contact Info

Product

Resources

About