Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities

Abstract

Although osteoarthritis (OA), a degenerative joint disease characterized by the degradation of joint articular cartilage and subchondral bones, is generally regarded as a degenerative rather than inflammatory disease, recent studies have indicated the involvement of inflammation in OA pathogenesis. Tabebuia avellanedae has long been used to treat various diseases; however, its role in inflammatory response and the underlying molecular mechanisms remain poorly understood. In this study, the pharmacological effects of Tabetri (Tabebuia avellanedae ethanol extract (Ta-EE)) on OA pathogenesis induced by monoiodoacetate (MIA) and the underlying mechanisms were investigated using experiments with a rat model and in vitro cellular models. In the animal model, Ta-EE significantly ameliorated OA symptoms and reduced the serum levels of inflammatory mediators and proinflammatory cytokines without any toxicity. The anti-inflammatory activity of Ta-EE was further confirmed in a macrophage-like cell line (RAW264.7). Ta-EE dramatically suppressed the production and mRNA expressions of inflammatory mediators and proinflammatory cytokines in lipopolysaccharide-stimulated RAW264.7 cells without any cytotoxicity. Finally, the chondroprotective effect of Ta-EE was examined in a chondrosarcoma cell line (SW1353). Ta-EE markedly suppressed the mRNA expression of matrix metalloproteinase genes. The anti-inflammatory and chondroprotective activities of Ta-EE were attributed to the targeting of the nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1) signaling pathways in macrophages and chondrocytes.

Trichosanthes tricuspidata Lour. Methanol Extract Exhibits Anti-Inflammatory Activity by Targeting Syk, Src, and IRAK1 Kinase Activity

“…LPS induced NF-κB activation, which phosphorylates the IκB kinase complex and simultaneously degrades IκBα, which results in translocation of p50/p65 NF-κB subunits in the nucleus [45,46]. Moreover, ERK1/2, JNK, and p38 have been identified as upstream subfamilies which activate AP-1 cascades [47,48]. erefore, to assess whether NF-κB and AP-1 upstream signaling is involved in the anti-inflammatory effect of Tt-ME, we examined phosphorylation of IκBα, p85, and AKT with respect to NF-κB signaling (Figure 3(a)); ERK, JNK, and p38 with respect to AP-1 signaling; and JAK2 in a time-dependent manner (time ranging from 5 to 60 mins) (Figures 3(c) and 3(e)).…”

Unraveling Natural Products’ Role in Osteoarthritis Management—An Overview

“…Finally, it must be said that TabetriTM had also documented inhibitory effects on inflammatory mediators and proinflammatory cytokines in a dose-dependent manner. Although this product is referred to as the ethanol extract of Tabebuia avellanedae, as a commercial product, it did not fall in the scope of this review and was not further investigated [74].…”

Tabebuia impetiginosa: A Comprehensive Review on Traditional Uses, Phytochemistry, and Immunopharmacological Properties

“…T. impetiginosa has been used traditionally to treat cancer [ 24 ], obesity [ 25 ], depression [ 26 ], viral, fungal, and bacterial infections [ 27 ], and inflammatory symptoms such as pain [ 28 ], arthritis [ 15 ], colitis [ 29 ], and prostatitis since the Inca civilization. The Callawaya Tribe makes a concentrated tea out of the tree’s inner bark for treating skin inflammatory diseases [ 8 ].…”

“…T. impetiginosa can alter the expression of signaling molecules involved in the inflammation process, including nitric oxide (NO), prostaglandin (PGE 2 ), and leukotriene B 4 (LTB 4 ) [ 8 , 15 , 28 ]. NO is essential for maintaining homeostasis and protecting human hosts and provides immunosuppressive effects as well as immunopathological and immunoprotective activities [ 44 ].…”

“…Several categories of compounds have been isolated and identified from T. impetiginosa, principally quinones, flavonoids, naphthoquinones, and benzoic acids [ 9 , 10 , 11 , 12 ]. In recent years, many investigations have demonstrated that extracts or compounds isolated from T. impetiginosa reveal an extensive range of pharmacological activities such as anti-obesity, antifungal, anti-psoriatic, antioxidant, anti-inflammatory, and anti-cancer activities [ 4 , 7 , 13 , 14 , 15 , 16 , 17 , 18 ]. It is particularly prominent in immunopharmacology.…”