T598 and T601 phosphorylation sites of canine parvovirus NS1 are crucial for viral replication and pathogenicity

Miao, Bichen; Chen, Songbiao; Zhang, Xuezhi; Ma, Peipei; Ma, Mengyu; Chen, Caiyi; Zhang, Xiujuan; Chang, Lingling; Du, Qian; Huang, Yong; Tong, Dewen

doi:10.1016/j.vetmic.2021.109301

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…Limited literature is available on the NS gene of CPV-2 compared to the CPV-2 VP2 gene, and there is a lack of sequence data in GenBank for review. One recent NS1 study investigated T598 and T601 phosphorylation sites of NS1 and determined that both of these sites are crucial for viral replication and pathogenicity [ 65 ]. Further studies are needed to determine the contribution of the substitutions identified here to the biological properties of NS1.…”

Section: Discussionmentioning

confidence: 99%

Predominance and first complete genomic characterization of canine parvovirus 2b in Turkey

et al. 2022

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Viral enteritis is a significant threat to domestic dogs. The two primary pathogens that cause viral enteritis in dogs are canine coronavirus (CCoV) and canine parvovirus (CPV). In this study, we investigated the occurrence of CPV-2, CCoV, and canine circovirus coinfection by characterizing circulating subtypes of CPV-2 in faecal samples from symptomatic dogs admitted to veterinary clinics located in Ankara, Elazığ, Kayseri, and Kocaeli provinces of Turkey, between 2019 and 2022. Virus detection by PCR and RT-PCR revealed that CPV-2 was present in 48 (77.4%) samples, and no other agents were detected. Based on the occurrence of the codon GAT at positions 1276 to 1278 (coding for aspartate at residue 426) of VP2, all CPV-2 isolates were confirmed to be of the CPV-2b subtype. The complete genome sequences of two CPV-2b isolates showed a high degree of similarity to and phylogenetic clustering with Australian and East Asian strains/isolates. The predominant CPV strain circulating in the three different regions of Turkey was found to be a CPV-2b strain containing the amino acid substitutions at Y324I and T440A, which commonly contribute to immune escape. This is the first report of complete genomic analysis of CPV-2 isolates circulating in symptomatic domestic dogs in Turkey. The evolution of CPV-2 has raised questions about the efficacy of current vaccination regimes and highlights the importance of monitoring the emergence and spread of new CPV-2 variants. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-022-05509-4.

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Section: Discussionmentioning

confidence: 99%

Predominance and first complete genomic characterization of canine parvovirus 2b in Turkey

et al. 2022

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“…The CPV-2 NS1 protein has remained relatively conserved during the process of virus evolution. It is a phosphorylated protein with multiple functions, such as inducing apoptosis and promoting oncolysis [ 4 , 5 , 6 ]. The VP2 gene is 1755 bp in length and encodes 584 amino acids.…”

Section: Introductionmentioning

confidence: 99%

The Changes in Canine Parvovirus Variants over the Years

Hao

Xiao

et al. 2022

IJMS

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Canine parvovirus (CPV-2) is one of the most important pathogens in dogs, and despite the continual development of vaccines against CPV-2, CPV-2 is still circulating in the canine population. The CPV-2a/2b/2c variant has replaced the original CPV-2 virus and seems to exhibit accelerated transmission. Although CPV-2 infection has been frequently reported, no studies have summarized information of CPV-2 variants currently circulating worldwide. To track the evolution of CPV-2, we downloaded and analyzed all VP2 sequences from the NCBI database (from 1978 to 2022). We found that CPV-2c shows a tendency to replace CPV-2a as the new dominant variant in Asia, South America, North America and Africa. Additionally, CPV-2c, which is prevalent in most regions of Asia, carries two special mutations in VP2, A5G and Q370R, and has become a dominant mutation with spillover already occurring. In conclusion, this summary of the types of global epidemic variants provides new insight into the evolution of CPV-2 and raises awareness for blocking the spread of this virus. The spread of Asian-derived CPV-2c urgently needs to be further under surveillance.

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“…NS1 significantly influences the pathogenicity and cytotoxicity of canine parvovirus 2 [ 8 ]. Table 1 lists the amino acid mutations found in NS1 sequences.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, this study aims to provide explanation on viral behavior by attempting to understand some these substitutions. NS1 protein plays a crucial role in viral pathogenicity and cytotoxicity [ 8 , 18 ]. The mutations of N351K and N361S in NS1 were observed in all the South American samples within the analyzed set.…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequence analysis of CPV-2 isolates from 1998 to 2020

Sarabandi

Pourtaghi

2023

Virol J

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Canine parvovirus-2 (CPV-2) is a virus with worldwide spread causing canine gastroenteritis. New strains of this virus have unique characteristics and are resistant to some vaccine strains. Therefore, understanding the root causes of resistance has proven to be of increasing concern to many scientists. This study collected 126 whole genome sequences of CPV-2 subtypes with specific collection dates from the NCBI data bank. The whole genome sequences of CPV-2 collected from different countries were analyzed to detect the new substitutions and update these mutations. The result indicated 12, 7, and 10 mutations in NS1, VP1, and VP2, in that respective order. Moreover, the A5G and Q370R mutations of VP2 are the most common changes in the recent isolates of the CPV-2C subtype, and the new N93K residue of VP2 is speculated to be the cause of vaccine failure. To summarize, the observed mutations, which are increasing over time, causes several changes in viral characteristic. A comprehensive understanding of these mutations can lead us to control potential future epidemics associated with this virus more efficiently.

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T598 and T601 phosphorylation sites of canine parvovirus NS1 are crucial for viral replication and pathogenicity

Cited by 7 publications

References 32 publications

Predominance and first complete genomic characterization of canine parvovirus 2b in Turkey

Predominance and first complete genomic characterization of canine parvovirus 2b in Turkey

The Changes in Canine Parvovirus Variants over the Years

Whole genome sequence analysis of CPV-2 isolates from 1998 to 2020

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