Whole genome sequence analysis of CPV-2 isolates from 1998 to 2020

Abstract

Canine parvovirus-2 (CPV-2) is a virus with worldwide spread causing canine gastroenteritis. New strains of this virus have unique characteristics and are resistant to some vaccine strains. Therefore, understanding the root causes of resistance has proven to be of increasing concern to many scientists. This study collected 126 whole genome sequences of CPV-2 subtypes with specific collection dates from the NCBI data bank. The whole genome sequences of CPV-2 collected from different countries were analyzed to detect the new substitutions and update these mutations. The result indicated 12, 7, and 10 mutations in NS1, VP1, and VP2, in that respective order. Moreover, the A5G and Q370R mutations of VP2 are the most common changes in the recent isolates of the CPV-2C subtype, and the new N93K residue of VP2 is speculated to be the cause of vaccine failure. To summarize, the observed mutations, which are increasing over time, causes several changes in viral characteristic. A comprehensive understanding of these mutations can lead us to control potential future epidemics associated with this virus more efficiently.

Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells

“…In addition, studies have shown that the cellular DNA damage response triggered by the invading single-stranded parvovirus can induce cell cycle arrest, thereby creating a favorable environment for viral replication ( Chen and Qiu, 2010 ; Lou et al, 2012 ; Xu et al, 2019 ; Zhang et al, 2019 ; Dai et al, 2020 ). While as an important component of the viral capsid, the CPV VP2 protein not only determines the host range of the virus ( Hueffer and Parrish, 2003 ), but also serves as a key protein in inducing the host immune defense mechanism ( Sarabandi and Pourtaghi, 2023 ). It follows that VP2 protein plays a crucial role in the biological functions related to virus-host interactions.…”