T helper type cytokines in sepsis: time-shared variance and correlation with organ dysfunction and hospital mortality

Costa, Ramon Teixeira; Araújo, Orlei Ribeiro de; Brunialti, Milena Karina Coló; Assunção, Murilo; Azevedo, Luciano César Pontes; Freitas, Flávio Geraldo Rezende; Salomão, Reinaldo

doi:10.1016/j.bjid.2019.04.008

Cited by 14 publications

(11 citation statements)

References 39 publications

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“…However, the modulatory role of JKAP on Th1 and Th17 cell functions should be assessed by more animal or cell experiments. (2) As for the elevation of Th1 and Th17 proportions and their correlations with more severe disease in sepsis patients, it is reasonable because Th1 and Th17 cells play a role that promotes the disease progression in sepsis as reported by the previous studies [ 21 , 23 – 25 ].…”

Section: Discussionmentioning

confidence: 92%

“…Th1 cell and Th17 cell are two relatively established cells that play critical roles in sepsis. For instance, a study illustrates that Th1, Th2, and Th17 type cytokines, IL-6, IL-8, and IL-10 are increased but IL-12 is decreased in patients with sepsis than healthy individuals; IL-8 overexpression correlates with renal and cardiac function damage, and IL-10 is downregulated in survivors than deaths [ 21 ]. Moreover, JKAP is involved in the regulation of Th cell functions.…”

Section: Discussionmentioning

confidence: 99%

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The correlation between Jun N-terminal kinase pathway-associated phosphatase and Th1 cell or Th17 cell in sepsis and their potential roles in clinical sepsis management

Peng

2020

Ir J Med Sci

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Background We aimed to investigate the association between Jun N-terminal kinase (JNK) pathway-associated phosphatase (JKAP) and T helper type 1 (Th1) cell or Th17 cell, and their clinical values in sepsis patients. Methods Totally 125 sepsis patients and 100 healthy subjects as controls were included. Peripheral blood was extracted from each sepsis patient and each control, then serum and peripheral blood mononuclear cell (PBMC) were separated. JKAP and inflammatory cytokines were detected in serum by ELISA; Th1 cell or Th17 cell proportion was detected in PBMC using flow cytometry. Results JKAP level was downregulated while Th1 and Th17 cell proportions were upregulated in sepsis patients compared with controls. JKAP level negatively correlated with Th1 cell proportion in sepsis patients and controls, while was only negatively associated with Th17 cell proportion in sepsis patients but not in controls. In sepsis patients, JKAP level negatively associated with TNF-α, IL-1β, and IL-17 expressions. Meanwhile, JKAP level negatively but Th17 cell proportion positively correlated with acute physiology and chronic health evaluation II (APACHE II) and sequential organ failure assessment (SOFA) scores; however, Th1 cell proportion only positively associated with APACHE II score but not SOFA score. Additionally, JKAP level was reduced, while Th1 and Th17 cell proportions were increased in septic deaths compared with survivors. Multivariate logistic regression model disclosed that JKAP level and Th17 cell proportion independently predicted 28-day mortality. Conclusion Blood JKAP correlates with decreased Th1 and Th17 cells, also associates with reduced inflammatory cytokines, disease severity, and favorable outcome in sepsis patients.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

The correlation between Jun N-terminal kinase pathway-associated phosphatase and Th1 cell or Th17 cell in sepsis and their potential roles in clinical sepsis management

Peng

2020

Ir J Med Sci

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“…As the release of inflammatory factor may be related to the differentiation of Th17 and Treg subsets and apoptosis of lymphocytes [19,20], we went on to further detect Th17 and Treg subsets in rats by flow cytometry. We found that when miR-126 was overexpressed, compared with sepsis group, the number of Th17 subgroup decreased, while the number of Treg subgroup increased.…”

Section: The Differentiation Of Th17 and Treg Subsets In Sepsis Was Imentioning

confidence: 99%

Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis

Zou

Yang

et al. 2020

Inflammation

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To observe the inflammatory response, differentiation of Th17/Treg subsets and apoptosis of lymphocytes, by regulating miR-126 in lymphocytes of septic rats. After using cecal ligation and puncture to establish sepsis model, miR-126 mimic and miR-126 inhibitor were used to transfect lymphocytes of septic rats in vitro and in vivo. ELISA was used to detect TNF-α, IL-6, IL-17, and IL-10, the differentiation of Th17 and Treg was measured by flow cytometry, and apoptosis of lymphocytes was observed by fluorescence microscope; the changes of caspase signaling pathway were detected by immunofluorescence, PCR, and Western blotting. The result show that the expression of miR-126 increased in sepsis. After overexpression of miR-126, the release of TNF-α, IL-6, and IL-17 decreased; the release of IL-10 increased; T lymphocyte subsets differentiated toward Treg; caspase signaling pathway weakened; and lymphocyte of apoptosis decreased compared with sepsis group. While, after inhibition of miR-126, the release of TNF-α, IL-6, and IL-17 increased; the release of IL-10 decreased; T lymphocyte subsets differentiated toward TH17; caspase signaling pathway enhanced; and lymphocyte of apoptosis increased compared with sepsis group. Taken together, regulation of miR-126 can alter the inflammatory response, differentiation of T lymphocyte subsets, and apoptosis of lymphocytes in septic rats.

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“…Furthermore, these inflammatory cytokines are important components of the innate immune response, which have also been reported to participate in human SIRS/sepsis (22). A previous study found that the increased plasma level of IL-1β was negatively correlated with the progression of organ dysfunction and mortality in septic patients (45). Another study reported that IL-1β commonly underwent an early but transient burst of expression after being exposed to the stimulation of endotoxin (46).…”

Section: Discussionmentioning

confidence: 99%

Laminar Inflammation Responses in the Oligofructose Overload Induced Model of Bovine Laminitis

Ding

Jiang

et al. 2020

Front. Vet. Sci.

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Bovine laminitis causes substantial economic losses and animal welfare problems in dairy farms worldwide. Previously published studies have reported that the inflammatory response plays a central role in the pathogenesis of the disease. To our knowledge, inflammation associated with bovine laminitis induced by high levels of exposure to oligofructose (OF) has not been reported and characterized. In fact, the disease manifestations in this model closely approximate those of clinical laminitis. The objective of this study was to characterize the inflammatory response in OF-induced bovine laminitis. A total of 12 Chinese Holstein dairy heifers were utilized in this study. The heifers were randomly divided into two groups, treatment (n = 6) and control (n = 6). The treatment group heifers were administered OF solutions via a stomach tube (dose: 17 g/kg of body weight). Upon development of a lameness score of 2 with consecutive positive reactions in the same claw, they would be humanely euthanized. Control heifers were administered deionized water (dose: 2 L/100 kg of body weight) and humanely euthanized at 72 h. Real-time quantitative PCR (qPCR) assays were performed to determine the messenger RNA (mRNA) concentrations of inflammatory mediators in the lamellae. Concentrations of interleukin (IL)-1β, IL-6, IL-8, C-X-C motif chemokine ligand-1 (CXCL-1), macrophage cationic peptide-2 (MCP-2), E-selectin, intercellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase-1 (iNOS-1), and plasminogen activator inhibitor-1 (PAI-1) were significantly increased (P < 0.05) in the treatment group. No significant difference was found for tumor necrosis factor alpha (TNF-α), IL-10, CXCL-6, and MCP-1. These results demonstrated and characterized the laminar inflammatory response leading to the pathogenesis of bovine laminitis at the early stages.

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T helper type cytokines in sepsis: time-shared variance and correlation with organ dysfunction and hospital mortality

Cited by 14 publications

References 39 publications

The correlation between Jun N-terminal kinase pathway-associated phosphatase and Th1 cell or Th17 cell in sepsis and their potential roles in clinical sepsis management

The correlation between Jun N-terminal kinase pathway-associated phosphatase and Th1 cell or Th17 cell in sepsis and their potential roles in clinical sepsis management

Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis

Laminar Inflammation Responses in the Oligofructose Overload Induced Model of Bovine Laminitis

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