T-DNA Controlled and Plant Specific Metabolism inAgrobacterium tumefaciensTransformed Tobacco and Soybean Tissues

Abstract

· The endogenous lAA and cytokinin levels measured in Agrobacterium tumefaciens transformed tobacco and soybean tissues were correlated with the onc-T-DNA genes and their functional effect on turner growth and morphology Résumé.-Les taux endogènes d'AIA et de cytokinines dans des tissues de tabac et de soja transformés par Agrobacterium tumefaciens sont corrélés avec la présence des one-gènes du T-DNA et leurs effects sur la croissance et la morphologie des tumeurs.

Free and Conjugated Indoleacetic Acid (IAA) Contents in Transgenic Tobacco Plants Expressing the iaaM and iaaH IAA Biosynthesis Genes from Agrobacterium tumefaciens

“…transformed calli contained on average approximately twice as much free IAA and three times more IAA conjugate (Table I), and 2.5 times more total IAA (free plus conjugated). The increase in free IAA content in transformed tissue is 7 to 13 times lower than what has been reported from other Agrobacterium-mediated tumors in tobacco SR1 (17,18), although it should be noted that in these studies the entire pTiC58 T-DNA region was transferred, and genes of known or unknown function present on the T-DNA might affect expression of the IAA biosynthetic genes and/or the metabolic regulation of the hormone metabolism in the cell. Different culture conditions might also affect the IAA-level.…”