T-Cell Receptor Gene Rearrangement Detection in Suspected Cases of Cutaneous T-Cell Lymphoma

Chitgopeker, Pooja; Sahni, Debjani

doi:10.1038/jid.2014.73

Cited by 15 publications

(12 citation statements)

References 10 publications

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“…Such clonal inflation can be attributed to expansion within the memory compartment, caused by an uneven homeostatic proliferation of naive T-cell repertoire, or may be indicative of a T-cell tumor. For example, cutaneous T-cell lymphoma (CTCL) samples commonly have a high clonality value, since there is a single pathogenic T-cell clone largely predominating in skin lesions (Chitgopeker et al 2014). …”

Section: Overview Of Methodologymentioning

confidence: 99%

Research Techniques Made Simple: High-Throughput Sequencing of the T-Cell Receptor

Matos

Rie

Teunissen

2017

Journal of Investigative Dermatology

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High-throughput sequencing (HTS) of the T-cell receptor (TCR) has advanced rapidly in recent years, giving the unique opportunity to identify and quantify every distinct T-cell clone present within any biological sample. This sensitive and accurate technique provides the relative frequency of each individual clone within the full T-cell repertoire, allowing to study particular single T-cell clones of interest as well as the concomitant analysis of all different clones of the whole population. HTS is essential to expand our knowledge on the diversity of the TCR repertoire in homeostasis or pathologic conditions, as well as to understand the development and kinetics of antigen-specific T-cell responses that critically determine protective immunity (i.e. vaccination) and immune-related disorders (i.e. auto-immunity and cancer). Interestingly, HTS can be tailored for personalized medicine, having the potential to monitor therapeutic interventions and reveal prognostic and diagnostic biomarkers. In this article, we briefly review the methodology, advances and limitations of HTS, and describe emerging applications of this novel technique in the field of investigative dermatology, including studying the pathogenesis of T cells in allergic dermatitis and its application in diagnosis, early recurrence detection and therapy monitoring of cutaneous T-cell lymphoma.

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Section: Overview Of Methodologymentioning

confidence: 99%

Research Techniques Made Simple: High-Throughput Sequencing of the T-Cell Receptor

Matos

Rie

Teunissen

2017

Journal of Investigative Dermatology

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“…During T lymphocyte development, each of these cells becomes mature through unique TCR gene rearrangements (Chitgopeker and Sahni 2014). Rearrangement in gene segments is responsible for variable regions of the TCR and results in diversity among antigen-specific lymphocyte receptors.…”

Section: Different Tcr Gene Rearrangements In B and T All Correlated mentioning

confidence: 99%

Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

et al. 2015

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Acute lymphoblastic leukemia is a hematological malignancy of lymphoid progenitor cells associated with excessive proliferation of lymphocytes, and lymphocyte markers can be used for the diagnosis and assessment of disease. Cell surface receptors, including T cell receptor and immunoglobulin (on T and B cells, respectively), are among the most important lymphocyte markers. Gene segment variation, i.e., involvement of several genes in the expression of each receptor, is effective on extensive rearrangement of cell surface receptors. These receptors can be observed in both T-acute lymphoblastic leukemia (ALL) and precursor B-ALL cells. In this study, we have discussed T cell receptor (TCR) gene rearrangements in ALL. As leukemia cell proliferation originates from a unique clone, clone-specific rearrangement can be very helpful in diagnosis and detection of remaining malignant cells from among normal cells for minimal residual disease. On the other hand, each of the TCR genes is associated with translocation in ALL types, and the result of gene rearrangements can be used as prognosis markers.

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“…While the reason for this is unclear, one possible explanation is the recent increased capability of identifying this disease, as the majority of the initial diagnoses are now made at an early stage 2. Our diagnostic abilities have been aided, in no small part, by the advent of PCR testing for gene rearrangements of the T-cell receptor (TCR)3 which helps support the diagnosis of lymphoma by detecting a clonal T-cell population 4. Even subtle histological findings which might otherwise have been considered non-diagnostic can be correlated with TCR gene rearrangements, immunohistochemistry and clinical impression to more confidently arrive at a CTCL diagnosis 5…”

Section: Introductionmentioning

confidence: 99%

Specific TCR gene rearrangements in mycosis fungoides: does advanced clinical stage show a preference?

et al. 2018

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T-Cell Receptor Gene Rearrangement Detection in Suspected Cases of Cutaneous T-Cell Lymphoma

Cited by 15 publications

References 10 publications

Research Techniques Made Simple: High-Throughput Sequencing of the T-Cell Receptor

Research Techniques Made Simple: High-Throughput Sequencing of the T-Cell Receptor

Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

Specific TCR gene rearrangements in mycosis fungoides: does advanced clinical stage show a preference?

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