Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

Kavianpur, Maria; Shahjahani, Mohammad; Jaseb, Kaveh; Kasar, Siddha; Saki, Najmaldin

doi:10.1007/s00580-015-2207-8

Cited by 4 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Correspondingly, previous studies have shown that the results of TCR gene rearrangements could be prognosis markers in acute lymphoblastic leukemia (ALL). 36 Not only that, we observed a specific module of V-J genes using WGCNA method, 26 the relationship between highly correlated V-J genes in the module and clinical features were investigated ( Figure 3 ). As far as we know, this is the first study to identify the module of V-J genes usage contributing to the TCR repertoires associated with clinical pathogenic characteristics.…”

Section: Discussionmentioning

confidence: 93%

Evaluating the Potential of T Cell Receptor Repertoires in Predicting the Prognosis of Resectable Non-Small Cell Lung Cancers

Song

Chen²,

Shi

et al. 2020

Molecular Therapy - Methods & Clinical Development

View full text Add to dashboard Cite

For resectable cancer patients, a method that could precisely predict the risk of postoperative recurrence would be crucial for guiding adjuvant treatment. Since T cell receptor (TCR) repertoires had been shown to be closely related to the dynamics of cancers, here we enrolled a cohort of patients to evaluate the potential of TCR repertoires in predicting the prognosis of resectable non-small cell lung cancers. Specifically, TCRβ repertoires were analyzed in surgical tumor tissues and matched adjacent non-tumor tissues from 39 patients enrolled with resectable non-small cell lung cancer, through target enrichment and high-throughput sequencing. As a result, there are significant differences between the TCR repertories of tumor samples and those of matched adjacent non-tumor samples as evaluated by criteria like the number of clonotypes. In addition, TCR repertoires were significantly associated with a few clinical features, as well as somatic mutations. Finally, certain TCRβ variable-joining (V-J) pairings were featured to build a logistic regression model in predicting postoperative recurrence of resectable non-small cell lung cancers with a testing area under the receiver operating characteristic curve (AUC) of around 0.9. Thus, we hypothesize that TCR repertoires could be potentially used to predict prognosis after curative surgery for non-small cell lung cancer patients.

show abstract

Section: Discussionmentioning

confidence: 93%

Evaluating the Potential of T Cell Receptor Repertoires in Predicting the Prognosis of Resectable Non-Small Cell Lung Cancers

Song

Chen²,

Shi

et al. 2020

Molecular Therapy - Methods & Clinical Development

View full text Add to dashboard Cite

show abstract

“…The development of PCR-based approaches in the last years has facilitated the complex characterization and classification of malignant diseases, patient stratification and monitoring of minimal residual disease (MRD) [9]. Following flow-cytometric analysis of T or B-cell populations, PCR-based clonality assays are requested to identify whether the populations of cells are derived from clonal or polyclonal expansions [10,11].…”

mentioning

confidence: 99%

Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies

Trandafir¹,

Ivanov²,

Dragos³

et al. 2019

Rev. Chim.

View full text Add to dashboard Cite

The aim of this study was to use clonal gene rearrangements assays to discriminate between oligo-clonal or polyclonal reactive processes in cases that presented difficulty in diagnosis of B or T-acute lymphoblastic leukemia (ALL) due to abnormal phenotypes detected by flow-cytometry. Monoclonal B /T cells were detected by rearrangements of immunoglobulin (Ig) and T-cell receptors (TCR) in 10 patients. After DNA extraction from peripheral blood, multiplex PCR was used to amplify the Ig/TCR gene rearrangements followed by capillary electrophoresis, according to BIOMED 2 conditions. The evaluation of the results was carried out in the clinical and interdisciplinary (histomorphological, molecular and flow-cytometric) context. The presence of clonal rearrangements may indicate the existence of two neoplastic processes or a single clone with two gene rearrangements � one productive and one non-productive on the two alleles, considering the use of genomic DNA. By fragment analysis we identified B/T cells gene recombinations using multiplex PCR with primer sets that target each type of gene family. Using these molecular markers we can discriminate between monoclonal/polyclonal cells and follow a clonal marker throughout the disease evolution.

show abstract

Prognostic significance of aberrant expression of CD markers in acute lymphoblastic leukemia

Kavianpour

Ketabchi

Saki

2017

memo

View full text Add to dashboard Cite

Prognostic value and clinical significance of TCR rearrangements for MRD monitoring in ALL patients

Cited by 4 publications

References 54 publications

Evaluating the Potential of T Cell Receptor Repertoires in Predicting the Prognosis of Resectable Non-Small Cell Lung Cancers

Evaluating the Potential of T Cell Receptor Repertoires in Predicting the Prognosis of Resectable Non-Small Cell Lung Cancers

Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies

Prognostic significance of aberrant expression of CD markers in acute lymphoblastic leukemia

Contact Info

Product

Resources

About