T-Cell Receptor and Immunoglobulin Gene Rearrangements in Diagnosing Skin Disease

Wood, Gary S.

doi:10.1001/archderm.137.11.1503

Cited by 31 publications

(12 citation statements)

References 12 publications

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“…Also, this can be a false-negative result, due to poor sampling, i.e. taking the skin sample with small number of malignant T-cells, and there is a possibility that primers used in this study did not cover all possible TCR-g gene rearrangements (5,21,22).…”

Section: Patients With Idiopathic Erythroderma and Clinical Suspicionmentioning

confidence: 99%

T-Cell Receptor-G Gene Rearrangement Analysis in the Diagnosis of Patients with Erythroderma / Dijagnostički Značaj Analize Preuređenja Gena Gama Lanca T-Ćelijskog Receptora Kod Pacijenata Sa Eritrodermijom

Kandolf-Sekulović¹,

Cikota²,

Dinić³

et al. 2009

Serbian Journal of Dermatology and Venerology

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The diagnosis of erythroderma is challenging, since clinical, histopathological and immunophenotypic findings are insufficient to differentiate between inflammatory and lymphomatous erythroderma. Thus, multiplex PCR was used for T-cell receptor-g gene rearrangement analysis, in the skin and peripheral blood samples of 24 patients (20 men and 4 women) with erythroderma of varying origin, in order to estimate its diagnostic value. Cutaneous T-cell lymphoma was confirmed in 9, benign inflammatory dermatosis in 12, and idiopathic erythroderma and clonal dermatitis in 3 patients. In the group of patients with erythrodermic cutaneous T-cell lymphoma, the dominant clone was detected in the skin of 8/9, and in none of the patients with inflammatory dermatoses. A dominant clone was found in peripheral blood of 5/6 samples of patients with erythrodermic cutaneous T-cell lymphoma, and in 2/12 patients with inflammatory dermatosis. T-cell receptor-g gene rearrangement analysis is valuable in differentiation between inflammatory and lymphomatous erythroderma, thus substantially improving the diagnosis of patients with erythroderma.

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Section: Patients With Idiopathic Erythroderma and Clinical Suspicionmentioning

confidence: 99%

T-Cell Receptor-G Gene Rearrangement Analysis in the Diagnosis of Patients with Erythroderma / Dijagnostički Značaj Analize Preuređenja Gena Gama Lanca T-Ćelijskog Receptora Kod Pacijenata Sa Eritrodermijom

Kandolf-Sekulović¹,

Cikota²,

Dinić³

et al. 2009

Serbian Journal of Dermatology and Venerology

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“…The technique is dependent on the fact that T cells bear a unique antigen receptor on their cell surface that serves as a specific marker for that cell and its clonal progeny. If a cell undergoes neoplastic transformation, its TCR becomes a tumor marker specific to that cell lineage (Wood, 2001). Using PCR and high-resolution electrophoresis, a TCR clone can be demonstrated in up to 90% of skin biopsies in MF cases (Bottaro et al, 1994).…”

Section: Limitationsmentioning

confidence: 99%

“…This loss is replaced by the insertion of N nucleotides at the joins. The result is a unique V/N nucleotide/J structure that serves as a "fingerprint" for that particular T cell and all its clonal progeny (Wood, 2001;Wood et al, 1994).…”

Section: How Is Tcr Gene Rearrangement Analysis Performed?mentioning

confidence: 99%

“…The γ gene structure is also simpler, with only 12 variable segments compared with other TCR genes such as the TCR-β gene, which has over 60 variable segments. Thus, fewer sets of primers are needed to detect most of the possible gene rearrangements (Kandolf Sekulovic et al, 2007;Wood, 2001).…”

Section: How Is Tcr Gene Rearrangement Analysis Performed?mentioning

confidence: 99%

“…This highly sensitive tool enables earlier and more accurate diagnosis of CTCL. With further studies and greater understanding of the pathogenesis of cutaneous lymphomas, these tools may be used for more precise disease staging and the ability to provide better prognostic information for patients, thus allowing for more tailored treatment (Wood, 2001;Gellrich et al, 2000).…”

Section: A Major Advantage Of Using Pcr Compared With Southern Blottimentioning

confidence: 99%

See 2 more Smart Citations

T-Cell Receptor Gene Rearrangement Detection in Suspected Cases of Cutaneous T-Cell Lymphoma

Chitgopeker

Sahni

2014

Journal of Investigative Dermatology

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Molecular Diagnosis of Cutaneous Diseases

Karan¹,

Babb-Tarbox²,

Aboutalebi³

et al. 2005

Arch Dermatol

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T-Cell Receptor and Immunoglobulin Gene Rearrangements in Diagnosing Skin Disease

Cited by 31 publications

References 12 publications

T-Cell Receptor-G Gene Rearrangement Analysis in the Diagnosis of Patients with Erythroderma / Dijagnostički Značaj Analize Preuređenja Gena Gama Lanca T-Ćelijskog Receptora Kod Pacijenata Sa Eritrodermijom

T-Cell Receptor-G Gene Rearrangement Analysis in the Diagnosis of Patients with Erythroderma / Dijagnostički Značaj Analize Preuređenja Gena Gama Lanca T-Ćelijskog Receptora Kod Pacijenata Sa Eritrodermijom

T-Cell Receptor Gene Rearrangement Detection in Suspected Cases of Cutaneous T-Cell Lymphoma

Molecular Diagnosis of Cutaneous Diseases

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