T-Cell Clonality in Synovial Fluid From Rheumatoid Joints Before and After Culture in Interleukin-2

Karim, Sabeeha Naheed; Murphy, Elizabeth A.; Sturrock, R. D.; Goudie, R. B.

doi:10.1093/rheumatology/34.3.232

Cited by 10 publications

(6 citation statements)

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“…To date, TCRBV clonality studies in RA have yielded conflicting results. In some studies evidence of oligoclonality of SF T cells has been demonstrated [48–50], but in other studies no evidence of oligoclonality was found [51–53]. We have found three clonally expanded TCRBV subsets in the SF in each patient studied, with two of them (BV13·1/3 and BV22) being shared by the two patients.…”

Section: Discussionmentioning

confidence: 52%

Simultaneous analysis of T cell clonality and cytokine production in rheumatoid arthritis using three-colour flow cytometry

Bakakos

Pickard²,

Wong

et al. 2002

Clinical and Experimental Immunology

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SUMMARY In this study we examined the cytokine production by T cells and TCRVβ subsets in peripheral blood (PB) and synovial fluid (SF) from six RA patients and PB from 10 normal subjects, using three‐colour flow cytometry. In two RA subjects we assessed T cell clonality by RT PCR using TCRBV family‐specific primers and analysed the CDR3 (complementarity determining region 3) length by GeneScan analysis. A high percentage of IFN‐γ‐ and IL‐2‐ producing cells was observed among the PB T cells in both the RA patients and normal controls and among the SF T cells in RA patients. In contrast, the percentage of T cells producing IL‐4 and IL‐5 was small among PB T cells in both RA patients and normal controls and among SF T cells in RA patients. There was no significant difference in the production of IFN‐γ, IL‐2 and IL‐5 between the two compartments (PB and SF); however, there were significantly more IL‐4‐producing cells in SF. Molecular analysis revealed clonal expansions of four TCRBV families in SF of two of the RA patients studied: TCRBV6·7, TCRBV13·1 and TCRBV22 in one and TCRBV6·7, TCRBV21·3 and TCRBV22 in the second. These expansions demonstrated cytokine expression profiles that differed from total CD3+ cells, implying that T cell subsets bearing various TCR‐Vβ families may have the potential to modulate the immune response in RA patients.

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Section: Discussionmentioning

confidence: 52%

Simultaneous analysis of T cell clonality and cytokine production in rheumatoid arthritis using three-colour flow cytometry

Bakakos

Pickard²,

Wong

et al. 2002

Clinical and Experimental Immunology

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“…V ␤ 7 cells were usually not prominent in these studies, in which PCR primers were used to specifically detect the BV7 transcripts (a method that should detect all 3 BV7 genes but does not distinguish between the 3 different BV7 genes). In one study (52) using samples from Japanese patients with primary SS or with human T lymphotropic virus type I-associated SS, BV7 transcripts were elevated and clonally restricted. A conserved third complementarity-determining region motif (QDXG) was noted among these BV7 transcripts.…”

Section: Discussionmentioning

confidence: 99%

Genomic absence of the gene encoding T cell receptor V_β7.2 is linked to the presence of autoantibodies in Sjögren's syndrome

Manavalan¹,

Valiando²,

Reeves³

et al. 2004

Arthritis & Rheumatism

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Objective. It is not yet known whether the absence of certain T cell receptor V ␤ (TCRBV) genes (e.g., due to genomic deletion) has functional significance. We examined this question in relation to a known 21.6-kb insertion/deletion-related polymorphism (IDRP) in the human BV locus.Methods. New polymerase chain reaction (PCR) genotyping methods were used. Monoclonal antibodies to TCRBV gene products were used to confirm the absence of the relevant proteins. Patients with Sjögren's syndrome (SS) or systemic lupus erythematosus (SLE) were compared with normal controls with regard to TCR genotypes and serologic profiles.Results. There are 3 known haplotypes (I, D1, D2) and 6 possible genotypes related to the 21.6-kb IDRP. Novel PCR-based methods were used to define these genotypes. In subjects with deleted/deleted (D/D) genotypes, T cells could not express V ␤ 7.2 TCRs, as assayed with a new antibody specific for V ␤ 7.2. This was the sole significant difference between subjects without the insertion and those with either 1 or 2 copies. Surprisingly, we found that the D/D genotype was associated with primary SS, but only when pathogenic autoantibodies were present. Conclusion.These results suggest that T cells expressing TCRs with V ␤ 7.2 are protective against a pathogenic immune response in SS. Thus, genomic polymorphism of TCR genes (along with the correct HLA alleles) determines whether T cells can direct a pathogenic autoimmune response.In both human and mouse T cell receptor (TCR) gene loci, major genomic deletions exist. These deletions result in a contracted TCR repertoire, with certain TCR

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“…6,7,12 As such, the finding of an (oligo)clonal T-cell population in the synovial tissue of patients with RA or other inflammatory rheumatic diseases even in absence of a T-cell malignancy can occur. [20][21][22] Tachibana et al 12 reported the detection of an identical clone in synovial fluid and LN by SB analysis in a patient with T-zone lymphoma. However, up until now no primary skin lesions of patients with MF have been analyzed for direct comparison.…”

Section: Discussionmentioning

confidence: 99%

Clonal identity between skin and synovial tissue in a case of mycosis fungoides with polyarthritis

Sandberg¹,

Abdouni²,

Lam³

et al. 2004

Journal of the American Academy of Dermatology

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T-Cell Clonality in Synovial Fluid From Rheumatoid Joints Before and After Culture in Interleukin-2

Cited by 10 publications

References 0 publications

Simultaneous analysis of T cell clonality and cytokine production in rheumatoid arthritis using three-colour flow cytometry

Simultaneous analysis of T cell clonality and cytokine production in rheumatoid arthritis using three-colour flow cytometry

Genomic absence of the gene encoding T cell receptor V_β7.2 is linked to the presence of autoantibodies in Sjögren's syndrome

Clonal identity between skin and synovial tissue in a case of mycosis fungoides with polyarthritis

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T-Cell Clonality in Synovial Fluid From Rheumatoid Joints Before and After Culture in Interleukin-2

Cited by 10 publications

References 0 publications

Simultaneous analysis of T cell clonality and cytokine production in rheumatoid arthritis using three-colour flow cytometry

Simultaneous analysis of T cell clonality and cytokine production in rheumatoid arthritis using three-colour flow cytometry

Genomic absence of the gene encoding T cell receptor Vβ7.2 is linked to the presence of autoantibodies in Sjögren's syndrome

Clonal identity between skin and synovial tissue in a case of mycosis fungoides with polyarthritis

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Genomic absence of the gene encoding T cell receptor V_β7.2 is linked to the presence of autoantibodies in Sjögren's syndrome