T-cell–B-cell cooperation

Mitchison, N. A.

doi:10.1038/nri1334

Cited by 167 publications

(125 citation statements)

References 36 publications

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“…Efficient B cell responses are facilitated by expression of costimulators on T cells recognizing the antigen presented by the B cell, known as cognate T cell help. Noncognate, or bystander help, requires higher doses of antigen, colocalization of an activated T cell, an APC, and the relevant B cell and is less likely to lead to prolonged memory B cell responses (23). Our results suggest that presentation of lipid antigen by the same B cell that has had its BCR cross-linked recruits more effective cognate iNK T cell help.…”

Section: Discussionsupporting

confidence: 50%

NK T cells provide lipid antigen-specific cognate help for B cells

Leadbetter

Brigl²,

Illarionov³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

210

236

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Section: Discussionsupporting

confidence: 50%

NK T cells provide lipid antigen-specific cognate help for B cells

Leadbetter

Brigl²,

Illarionov³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

210

236

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“…Antibody detection was limited to antibodies of the IgG subtype. This approach led to the identification of 31 putative antigenic proteins capable of eliciting a T cell-dependent antibody response, as revealed by IgG production [42,43]. Similar approaches have been used recently to identify relevant antigenic proteins in oncologic, autoimmune, and infectious diseases [44][45][46][47][48][49][50].…”

Section: Discussionmentioning

confidence: 99%

Immunoproteomic identification of bovine pericardium xenoantigens

et al. 2008

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Bovine pericardium is an important biomaterial with current application in glutaraldehyde-fixed bioprosthetic heart valves and possible future application as an unfixed biological scaffold for tissue engineering. The importance of both humoral and cell-mediated rejection responses toward fixed and unfixed xenogeneic tissues has become increasingly apparent. However, the full scope and specific identities of bovine pericardium proteins that can elicit an immune response remain largely unknown. In this study, an immunoproteomic approach was used to survey bovine pericardium proteins for their ability to elicit a humoral immune response in rabbits. A two-stage protein extraction protocol was used to separate bovine pericardium proteins into water-and lipid-soluble fractions. Two-dimensional gel electrophoresis was performed to separate the proteins from each fraction. Western blots were generated from two-dimensional gels of both bovine pericardium protein fractions. These blots were probed with serum from rabbits immunized with bovine pericardium and a secondary antibody was used to assess for IgG positivity. Western blots were compared to duplicate two-dimensional gels and proteins in matched spots were identified by tandem mass spectrometry. Thirty-one putative protein antigens were identified, eight of which are known to be antigenic from previous studies. All of the putative antigens demonstrated progressive staining intensity with increasing days of post-exposure serum. Identified antigenic proteins represented a variety of functional and structural protein types, and included both cellular and matrix proteins. The results of this study have implications for the use of bovine pericardium as a biomaterial in bioprostheses and tissue engineering applications, as well as xenotransplantation in general.

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“…B cells were shown to be the source of the specific Abs, but the role of Ag-specific T cells in the Ab response remained mysterious. This history is nicely reviewed by Mitchison (7).…”

mentioning

confidence: 99%

The Carrier Effect and T Cell/B Cell Cooperation in the Antibody Response

Parker

2013

The Journal of Immunology

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T-cell–B-cell cooperation

Cited by 167 publications

References 36 publications

NK T cells provide lipid antigen-specific cognate help for B cells

NK T cells provide lipid antigen-specific cognate help for B cells

Immunoproteomic identification of bovine pericardium xenoantigens

The Carrier Effect and T Cell/B Cell Cooperation in the Antibody Response

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