T and B cell populations in blood and lymph node in lymphoproliferative disease

Cooper, David A.; Petts, Valerie; Luckhurst, E; Biggs, J. C.; Penny, Ronald

doi:10.1038/bjc.1975.96

Cited by 17 publications

(7 citation statements)

References 15 publications

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“…The present investigations confirm the opinion that most lymphoproliferative diseases of the non-Hodgkin type represent a variety of disorders in the differentia tion of the B cell series [6,17,18], whereas lymphoma classified as T cell in origin are very rare.…”

Section: Discussionsupporting

confidence: 88%

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Studies on the Use of Membrane Markers for the Differential Diagnosis of Malignant Lymphomas

et al. 1979

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Lymphoid cells isolated from lymph nodes, spleen and the peripheral blood were examined using the rosette test with sheep erythrocytes (E), immunofluorescent staining of the surface immunoglobulins (Smlg) and the combined test (Smlg+E). The studies were performed on 48 patients with untreated non-Hodgkin lymphoma, 14 Hodgkin patients, 5 patients with lymphadenitis and on 132 controls. In the control group the following percentages of B-T lymphocytes were obtained: blood (n=100): T-68 ± 9, B-20 ± 6, 0-11 ± 7, BT-1 + 1, lymph nodes (n=24): T-62 ± 13, B-19 ± 11, 0-18 ± 8, BT-1 ± 1, spleens (n=8): T-45 ± 9, B-33 ± 9, 0-23 ± 7, BT-1 ± 1. In non-Hodgkin lymphoma lymph nodes a statistically significant increase of B and ‘null’ cells was noted in comparison to controls and to the Hodgkin lymphoma group. The characteristics of the Hodgkin nodes did not differ significantly from controls. After arranging the non-Hodgkin lymphomas according to the Kiel classification it was observed that in the low-grade malignant lymphomas subtype most often an immunological ‘B type’ and ‘mixed B/0’ type was present whereas the high-grade malignant lymphoma group showed a distinct majority of ‘null’ cells. It was concluded that the surface markers studied allow a differentiation in most cases between the non-Hodgkin lymphomas and other disorders of the lymphoid system. They are not sufficient to distinguish the subtypes of non-Hodgkin lymphomas but do give interesting information about the more exact nature of the disorder.

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Section: Discussionsupporting

confidence: 88%

“…The results obtained from the blood lymphocytes of healthy donors are within the normal range found by others [6,20]. In our study, surface marker charac teristics of lymphocytes from normal lymph nodes and normal blood were very similar and indicate a distint T cell predominance [10].…”

Section: Discussionsupporting

confidence: 86%

Studies on the Use of Membrane Markers for the Differential Diagnosis of Malignant Lymphomas

et al. 1979

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“…The Fc-receptor-bearing lym phocyte population on the other hand was less than that found in peripheral blood (23 ± 4°/o) but the C3-receptor-bearing popu lation was on average higher than that found in peripheral blood (33 ± 6%) and in all but one case was always higher than the corresponding value for the Fc population. Mavligit et al [1975] reported their find ings in normal mesenteric nodes from a pa- Cooper et al [1975] in their study of T and B cell populations in blood and lymph node in lymphoproliférative disease report ed a T cell count of 26 and 63% in two nor mal nodes removed during elective surgery. The corresponding Fc-receptor value was 3 and 21%.…”

Section: Discussionmentioning

confidence: 99%

“…Several reports dealing with thymus, tonsil [Brown and Greaves, 1974a, b], bone marrow [Borella and Sen, 1974] and spleen [Habeshaw and Stuart, 1974;Kaur et al, 1974] have re cently appeared in the literature, and also a few studies dealing with surface markers on malignant lymphocytes in lymph nodes and spleens of patients with lymphoproliférative diseases [Peter et al, 1974;Cooper et al, 1975]. Reports of lymphocyte subpopula tions in normal lymph nodes [Papamichael et al, 1971;Verma et al, 1971] or in re gional nodes draining solid tumours [7iafc-raklides et al, 1974;Richters and Kasper sky, 1975] have, however, been rather in frequent.…”

Section: Introductionmentioning

confidence: 99%

T and B Lymphocyte Populations in Human Normal Lymph Node, Regional Tumour Lymph Node and Inflammatory Lymph Node

Eremin¹,

Plumb²,

Coombs³

1976

Int Arch Allergy Immunol

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We have determined the T and B lymphocyte subpopulations in normal human lymph nodes. The lymphocyte profile was the same irrespective of the anatomical distribution and was similar to that found in peripheral blood with identical T and B cell values but with a lower Fc and a higher C₃-receptor-bearing lymphocyte subpopulation. This pattern showed a marked change in the regional nodes of patients with mammary carcinoma and nodes draining a variety of other solid tumours, with a fall in T and pronounced elevation of B, Fc and C₃ cells but with a persistence of C₃ predominance. The lymphocyte profile found in tonsils and nodes draining inflammatory foci was a similar but further exaggeration of the tumour node pattern, with reversal of T and B cell ratios. The T and B lymphocyte percentages in the peripheral blood of patients with clinically localized breast cancer are identical to those of the healthy controls. Different Fc/C₃ subsets exist in peripheral blood and lymphoid structures and probably represent a differential functional heterogeneity. Proximity of tumour to the draining node modified this profile.

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“…The second is to assess the maturity of the B cell by a number of cell surface markers, and the third is to relate the surface markers to the histology of the tumour. Previous attempts to identify the cells present in human lymphomas by surface marker techniques (Brouet, Labaume and Seligmann, 1975;Cooper et al, 1975;Dorfman, 1975;Gajl-Peezalska, Bloomfield and Sosin, 1975;Habeshaw and Stuart, 1975;Jaffe et al, 1975;Huang et al, 1974;Peter, Mackenzie and Glassy, 1974;Stuart and Habeshaw, 1976;Smith et al, 1973) have all shown that most non-Hodgkin lymphomas consist predominantly of B cells, with a minority of T cells. A few are derived from T cells, and those arising from macrophages are distinctly uncommon.…”

mentioning

confidence: 99%

Correlation of surface receptors with histological appearance in 29 cases of non-Hodgkin lymphoma

Habeshaw¹,

Macaulay²,

Stuart³

1977

Br J Cancer

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Summary.-The receptor patterns of cell suspensions from 29 cases of non-Hodgkin lymphoma were correlated with the histology of the nodes from which the cells were taken. Twenty-two were judged to be predominantly or largely B-cell, and because of this preponderance these were divided by a method based on the distribution of surface immunoglobulin and the expression of Fc and C3 receptors." Mature " B-cell and B-mixed tumours showing capping surface Ig with Fc and/or C3 receptors correlated well with a nodular growth pattern, and consisted of what Rappaport (1966) calls "poorly differentiated" lymphocytes equivalent to the "small cleaved " cell as defined by Lukes and Collins (1975). Ten of the 14 patients in this receptor category are alive between 12 and 30 months after diagnosis.Receptor-silent and " immature " B-cell tumours with non-capping surface Ig correlated predominantly with the Rappaport histiocytic lymphoma and Lukes and Collins' large cleaved and large non-cleaved lymphomas, though these histological categories also included a wide variety of other receptor types such as T-cell, Receptor-overlap and the single true Macrophage tumour. Five of the 11 patients with receptor-silent or immature B-cell tumours are alive between 7 and 15 months after the diagnosis.Diffuse mixed and diffuse poorly differentiated lymphocytic lymphomas in Rappaport's classification correlated poorly with receptors, mature and immature B-cell tumours being equally represented.

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T and B cell populations in blood and lymph node in lymphoproliferative disease

Cited by 17 publications

References 15 publications

Studies on the Use of Membrane Markers for the Differential Diagnosis of Malignant Lymphomas

Studies on the Use of Membrane Markers for the Differential Diagnosis of Malignant Lymphomas

T and B Lymphocyte Populations in Human Normal Lymph Node, Regional Tumour Lymph Node and Inflammatory Lymph Node

Correlation of surface receptors with histological appearance in 29 cases of non-Hodgkin lymphoma

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