Correlation of surface receptors with histological appearance in 29 cases of non-Hodgkin lymphoma

Abstract

Summary.-The receptor patterns of cell suspensions from 29 cases of non-Hodgkin lymphoma were correlated with the histology of the nodes from which the cells were taken. Twenty-two were judged to be predominantly or largely B-cell, and because of this preponderance these were divided by a method based on the distribution of surface immunoglobulin and the expression of Fc and C3 receptors." Mature " B-cell and B-mixed tumours showing capping surface Ig with Fc and/or C3 receptors correlated well with a nodular growth pattern, and consisted of what Rappaport (1966) calls "poorly differentiated" lymphocytes equivalent to the "small cleaved " cell as defined by Lukes and Collins (1975). Ten of the 14 patients in this receptor category are alive between 12 and 30 months after diagnosis.Receptor-silent and " immature " B-cell tumours with non-capping surface Ig correlated predominantly with the Rappaport histiocytic lymphoma and Lukes and Collins' large cleaved and large non-cleaved lymphomas, though these histological categories also included a wide variety of other receptor types such as T-cell, Receptor-overlap and the single true Macrophage tumour. Five of the 11 patients with receptor-silent or immature B-cell tumours are alive between 7 and 15 months after the diagnosis.Diffuse mixed and diffuse poorly differentiated lymphocytic lymphomas in Rappaport's classification correlated poorly with receptors, mature and immature B-cell tumours being equally represented.

Morphology of lymphatic cells and of their derived tumours.

“…In contrast to B-CLL lymphocytes, the small follicular centre cells of cases with leukaemic manifestations and either a nodular or diffuse structure show a lesser tendency to form rosettes with mouse erythrocytes (Koziner et al, 1977). Quite conceivably, the cells of diffuse centroblasticcentrocytic ML with anaplasia may behave as 'receptor-silent' cells or show immature receptor profiles, as has been reported (Habeshaw et al, 1977) for some diffuse, mixed, and histiocytic ML, which correspond to large cleaved and non-cleaved follicular centre cell ML described by Lukes and Collins (1974). It has been postulated that for the characterisation of centroblastic-centrocytic ML, further investigation of the tissue between the neoplastic follicles may yield significant additional information.…”

Terminal deoxynucleotidyl transferase activity in lymphoma

“…After mononuclear cell separation, the cells in each case were surface-marked using sheep erythrocytes for E, using ox red cells for Fe and C3 receptors, and for surfacemembrane immunoglobulin (SIg). Using polyvalent anti-human G, M and A heavy-chain antisera, the proportions of capping and noncapping cells were determined after appropriate incubation (Habeshaw et al, 1977 for up to 3 weeks before TdT estimation as previously described (Hoffbrand et al, 1977).…”

“…The remaining tumours were mainly (over 80%) or entirely expressing monoclonal surface globin (Table III) (Habeshaw et al, 1977).…”

Cell receptor studies on six anaplastic tumours of the thyroid.

“…It is noteworthy that this tumour was associated with a follicular or 'nodular' lymphoma in a cervical node. Half of the 'nodular' lymphomas in the series of Habeshaw et al (1977) had a receptor pattern similar to reactive nodes, neither T nor B cells predominating.…”

“…In a previously reported series of non-Hodgkin malignant lymphoma, Habeshaw et al (1977) subdivided the B lymphocyte lymphomas on the basis of two distinct patterns of surface immunofluorescence. Surface immunoglobulin may be either aggregated at one pole of the cell (capping fluorescence) or distributed diffusely over the entire cell surface (noncapping fluorescence).…”