In this paper, we study a system of nonlinear Riemann-Liouville fractional ordinary differential equations with parameters, subject to coupled multi-point boundary conditions which contain fractional derivatives. By using some properties of the associated Green's functions and the Guo-Krasnosel'skii fixed point theorem, we prove the existence of positive solutions for this problem when the parameters belong to various intervals. Then, we present sufficient conditions for the nonexistence of positive solutions.Key words: Fractional differential equations, multi-point boundary conditions, positive solutions, existence, nonexistence.for all = 1, … , ( ∈ ℕ), 0 < 1 < ⋯ < ≤ 1, , > 0, and 0+ denotes the Riemann-Liouville derivative of order (for = , , 1 , 2 , 1 , 2 ). Under some assumptions on the nonnegative functions and , we present intervals for the parameters and such that positive solutions of (S)-(BC) exist. By a positive solution of problem (S)-(BC)we mean a pair of functions ( , ) ∈ ( ([0,1]), [0, ∞))) 2 satisfying ( ) and ( ), with ( ) > 0 for all Lemma 2.1 If ≠ 0 and , ∈ (0,1) ∩ 1 (0,1), then the pair of functions ( , ) ∈ [0,1] × [0,1] given by