Systemic human orbital fat-derived stem/stromal cell transplantation ameliorates acute inflammation in lipopolysaccharide-induced acute lung injury

Chien, Ming Hsien; Bien, Mauo Ying; Ku, Chia-Chi; Chang, Yin Yi; Pao, Hsiang Yin; You, Yang; Hsiao, Michael; Chen, Chi Long; Ho, Jennifer H.

doi:10.1097/ccm.0b013e31823bc89a

Cited by 52 publications

(64 citation statements)

References 55 publications

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“…Interestingly, one recent study has shown that, as compared to a lower dose of ADMSC, the higher dose of ADMSC did not offer an additional benefit on attenuating lung injury in the sepsis syndrome setting [29]. In addition, the results of the resent study demonstrated that treatment with three doses of H-ADMSC was inconsistent in suppressing the inflammatory mediators.…”

Section: Resultsmentioning

confidence: 99%

Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in rats

et al. 2013

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IntroductionWe tested the hypothesis that apoptotic adipose-derived mesenchymal stem cells (A-ADMSC) are superior to healthy (H)-ADMSC in attenuating cecal ligation puncture (CLP)-induced sepsis-mediated lung and kidney injuries.MethodsAdult male rats divided into group 1 (sham controls), group 2 (CLP), group 3 [CLP + H-ADMSC administered at 0.5, 6, and 18 hours after CLP], and group 4 [CLP + A-ADMSC administered as in group 3] were sacrificed 72 hours after CLP with blood, lung, and kidney collected for studies.ResultsWhite blood cell (WBC) count, circulating TNF-α and creatinine levels were higher in groups 2 and 3 than in groups 1 and 4 (all P < 0.001). Kidney and lung damage scores were highest in group 2, lowest in group 1, significantly higher in group 3 than in group 4 (all P < 0.0001). Protein expressions of inflammatory (ICAM-1, MMP-9, TNF-α, NF-κB), oxidative, and apoptotic (Bax, caspase-3, PARP) biomarkers were higher in groups 2 and 3 than groups 1 and 4, whereas anti-apoptotic (Bcl-2) and mitochondrial integrity (cytochrome-C) biomarkers were lower in groups 2 and 3 than in groups 1 and 4 (all P < 0.001). Expressions of anti-oxidant biomarkers at protein (GR, GPx, NQO-1, HO-1) and cellular (GR, GPx) levels were highest in group 4 (all P < 0.001). The number of inflammatory cells (CD3+) in lungs and levels of DNA damage marker (γ-H2AX) in kidneys were higher in groups 2 and 3 than in groups 1 and 4 (all P < 0.001).ConclusionsA-ADMSC therapy was superior to H-ADMSC therapy in protecting major organs from damage in rats with CLP-induced sepsis syndrome.

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Section: Resultsmentioning

confidence: 99%

Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in rats

et al. 2013

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“…In our previous study, the optimal therapeutic dosage of OFSCs for transplantation including acute tissue injury was 3 × 10 7 cells/kg bodyweight [15,18]. In this study, 3 × 10 4 cells in 20 μl phosphate-buffered saline (PBS) was chosen as the therapeutic dosage based on the weight of one testis, and local injection of 20 μl PBS served as control.…”

Section: Orbital Fat-derived Stem Cell Transplantationmentioning

confidence: 99%

“…Orbital fat-derived stem cells (OFSCs) are MSCs isolated from human orbital fat tissue [14] and their therapeutic effects on acute tissue injury have been demonstrated via paracrine tissue support, immunomodulation, and differentiation ability in our previous experimental studies [15][16][17]. In this study, rats received surgery with 720°of unilateral testicular torsion for 3 hours, and local injection of OFSCs 30 minutes before surgical detorsion was performed.…”

Section: Introductionmentioning

confidence: 99%

Local Injection of Mesenchymal Stem Cells Protects Testicular Torsion-induced Germ Cell Injury

Hsiao

Chang

et al. 2016

Cytotherapy

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“…COPD comprises two major phenotypes which are chronic bronchitis and emphysema. Interestingly, it has been demonstrated that mouse MSCs are able to ameliorate the emphysematous changes and reduce destruction in elastase-induced emphysema model through upregulation Systemic orbital fat-derived stem/stromal cells are effective in modulating inflammation [69] 8…”

Section: Therapy Approaches Of Mscs For Ards and Copdmentioning

confidence: 99%

Protective Effects of Ellagic Acid against UVA-induced Oxidative Stress in Human Dermal Papilla

Kim¹,

Han²,

An³

et al. 2016

Asian J Beauty Cosmetol

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MSCs have become an emerging cell source with their immune modulation, high proliferation rate, and differentiation potential; indeed, they have been challenged in clinical trials. Recently, it has shown that ROS play a dual role as both deleterious and beneficial species depending on their concentration in MSCs. Various environmental stresses-induced excessive production of ROS triggers cellular senescence and abnormal differentiation on MSCs. Moreover, MSCs have been suggested to participate in the treatment of ALI/ARDS and COPD as a major cause of high morbidity and mortality. Therapeutic mechanisms of MSCs in the treatment of ARDS/COPD were focused on cell engraftment and paracrine action. However, ROS-mediated therapeutic mechanisms of MSCs still remain largely unknown. Here, we review the key factors associated with cell cycle and chromatin remodeling to accelerate or delay the MSC aging process. In addition, the enhanced ROS production and its associated pathophysiological pathways will be discussed along with the MSC senescence process. Furthermore, the present review highlights how the excessive amount of ROS-mediated oxidative stress might interfere with homeostasis of lungs and residual lung cells in the pathogenesis of ALI/ARDS and COPD.

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Systemic human orbital fat-derived stem/stromal cell transplantation ameliorates acute inflammation in lipopolysaccharide-induced acute lung injury

Cited by 52 publications

References 55 publications

Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in rats

Apoptotic adipose-derived mesenchymal stem cell therapy protects against lung and kidney injury in sepsis syndrome caused by cecal ligation puncture in rats

Local Injection of Mesenchymal Stem Cells Protects Testicular Torsion-induced Germ Cell Injury

Protective Effects of Ellagic Acid against UVA-induced Oxidative Stress in Human Dermal Papilla

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