Systemic and mucosal antibody response of freshwater cultured Asian seabass (Lates calcarifer) to monovalent and bivalent vaccines against Streptococcus agalactiae and Streptococcus iniae

Lan, Nguyen Giang Thu; Salin, Krishna R.; Longyant, Siwaporn; Senapin, Saengchan; Dong, Ha Thanh

doi:10.1016/j.fsi.2020.11.014

Cited by 38 publications

(29 citation statements)

References 26 publications

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“…However, to improve the efficacy of the vaccine, a booster dose(s) is often required in order to obtain high antibody titres against the pathogen (Angelidis, 2006;Bøgwald & Dalmo, 2019;Thu Lan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of heat‐killed and formalin‐killed vaccines against Tilapia tilapinevirus in juvenile Nile tilapia (Oreochromis niloticus)

Mai

Kayansamruaj

Taengphu

et al. 2021

Journal of Fish Diseases

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Tilapia tilapinevirus (also known as tilapia lake virus, TiLV) is considered to be a new threat to the global tilapia industry. The objective of this study was to develop simple cell culture-based heat-killed (HKV) and formalin-killed (FKV) vaccines for the prevention of disease caused by TiLV. The fish were immunized with 100 µl of either HKV or FKV by intraperitoneal injection with each vaccine containing 1.8 × 10 6 TCID 50-inactivated virus. A booster vaccination was carried out at 21-day postvaccination (dpv) using the same protocol. The fish were then challenged with a lethal dose of TiLV at 28 dpv. The expression of five immune genes (IgM, IgD, IgT, CD4 and CD8) in the head kidney and spleen of experimental fish was assessed at 14 and 21 dpv and again after the booster vaccination at 28 dpv. TiLV-specific IgM responses were measured by ELISA at the same time points. The results showed that both vaccines conferred significant protection, with relative percentage survival of 71.3% and 79.6% for HKV and FKV, respectively. Significant up-regulation of IgM and IgT was observed in the head kidney of fish vaccinated with HKV at 21 dpv, while IgM, IgD and CD4 expression increased in the head kidney of fish receiving FKV at the same time point. After booster vaccination, IgT and CD8 transcripts were significantlyThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Section: Introductionmentioning

confidence: 99%

Efficacy of heat‐killed and formalin‐killed vaccines against Tilapia tilapinevirus in juvenile Nile tilapia (Oreochromis niloticus)

Mai

Kayansamruaj

Taengphu

et al. 2021

Journal of Fish Diseases

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“…Although the pathway of IgM secretion in the mucosal compartment (mucus) is unclear, it is possible that mucosal antibodies are produced locally in the mucosa-associated lymphoid organs and/or by the systemic immune system (Esteban & Cerezuela, 2015;Koppang et al, 2015;Salinas et al, 2011;Salinas & Parra, 2015;Salinas et al, 2021). In other research using Asian seabass, monovalent and bivalent bacterial vaccines induced both systemic and mucosal IgM (Thu- Lan et al, 2021). Similar kinetics have been reported for IgM secretion in the serum of red hybrid tilapia, infected IP with TiLV (Tattiyapong et al, 2020).…”

Section: Hkv and Fkv Induce Both Systemic And Mucosal Igmmentioning

confidence: 99%

“…Ideally, when a vaccine is administered, the fish’s immune response is stimulated to produce of antibodies and an immunologic memory against the pathogen (Secombes & Belmonte, 2016), so that the immune system responses more effectively if the fish should encounter the pathogen at a late date. However, to improve the efficacy of the vaccine, a booster dose(s) is often required in order to obtain high antibody titers against the pathogen (Angelidis, 2006; Bogwald & Dalmo, 2019; Thu Lan et al, 2021). Inactivated vaccines normally stimulate humoral immune responses, involving helper T cells (CD4+ T cells) and antibody-secreting B cells, secreting IgM, IgD or IgT (Smith et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of heat-killed and formalin-killed vaccines againstTilapia tilapinevirusin juvenile Nile tilapia (Oreochromis niloticus)

Mai

Kayansamruaj

Taengphu

et al. 2021

Preprint

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Tilapia tilapinevirus (also known as tilapia lake virus, TiLV) is considered to be a new threat to the global tilapia industry. The objective of this study was to develop simple cell culture-based heat-killed (HKV) and formalin-killed (FKV) vaccines for the prevention of disease caused by TiLV. The fish were immunized with 100 μL of either HKV or FKV by intraperitoneal injection with each vaccine containing 1.8 × 106 TCID50 inactivated virus. A booster vaccination was carried out at 21-day post vaccination (dpv) using the same protocol. The fish were then challenged with a lethal dose of TiLV at 28 dpv. The expression of five immune genes (IgM, IgD, IgT, CD4 and CD8) in the head kidney and spleen of experimental fish was assessed at 14 and 21 dpv and again after the booster vaccination at 28 dpv. TiLV-specific IgM responses were measured by ELISA at the same time points. The results showed that both vaccines conferred significant protection, with relative percentage survival (RPS) of 71.3% and 79.6% for HKV and FKV, respectively. Significant up-regulation of IgM and IgT was observed in the head kidney of fish vaccinated with HKV at 21 dpv, while IgM, IgD and CD4 expression increased in the head kidney of fish receiving FKV at the same time point. After booster vaccination, IgT and CD8 transcripts were significantly increased in the spleen of fish vaccinated with the HKV, but not with FKV. Both vaccines induced a specific IgM response in both serum and mucus. In summary, this study showed that both HKV and FKV are promising injectable vaccines for the prevention of disease caused by TiLV in Nile tilapia.

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“…Fish mortality was recorded for 14 days. The protective efficacy of the chimeric EiCh protein was evaluated as cumulative per cent mortality (CPM) of each treatment as well as relative per cent survival (RPS) (Lan et al, 2021).…”

Section: Bacterial Challenge In Immunized Fishmentioning

confidence: 99%

A multi‐epitope chimeric protein elicited a strong antibody response and partial protection against Edwardsiella ictaluri in Nile tilapia

Machimbirike

Pornputtapong

Senapin

et al. 2021

Journal of Fish Diseases

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Edwardsiella ictaluri infects several fish species and protection of the all the susceptible fish hosts from the pathogen using a monovalent vaccine is impossible because the species is composed of host-based genotypes that are genetic, serological and antigenic heterogenous. Here, immunoinformatic approach was employed to design a cross-immunogenic chimeric EiCh protein containing multi-epitopes. The chimeric EiCh protein is composed of 11 B-cell epitopes and 7 major histocompatibility complex class II epitopes identified from E. ictaluri immunogenic proteins previously reported.The 49.32 kDa recombinant EiCh protein was expressed in vitro in Escherichia coli BL-21 (DE3) after which inclusion bodies were successfully solubilized and refolded.Ab initio protein modelling revealed secondary and tertiary structures. Secondary structure was confirmed by circular dichroism spectroscopy. Antigenicity of the chimeric EiCh protein was exhibited by strong reactivity with serum from striped catfish and Nile tilapia experimentally infected with E. ictaluri. Furthermore, immunogenicity of the chimeric EiCh protein was investigated in vivo in Nile tilapia juveniles and it was found that the protein could strongly induce production of specific antibodies conferring agglutination activity and partially protected Nile tilapia juveniles with a relative survival percentage (RPS) of 42%. This study explored immunoinformatics as reverse vaccinology approach in vaccine design for aquaculture to manage E. ictaluri infections.

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Systemic and mucosal antibody response of freshwater cultured Asian seabass (Lates calcarifer) to monovalent and bivalent vaccines against Streptococcus agalactiae and Streptococcus iniae

Cited by 38 publications

References 26 publications

Efficacy of heat‐killed and formalin‐killed vaccines against Tilapia tilapinevirus in juvenile Nile tilapia (Oreochromis niloticus)

Efficacy of heat‐killed and formalin‐killed vaccines against Tilapia tilapinevirus in juvenile Nile tilapia (Oreochromis niloticus)

Efficacy of heat-killed and formalin-killed vaccines againstTilapia tilapinevirusin juvenile Nile tilapia (Oreochromis niloticus)

A multi‐epitope chimeric protein elicited a strong antibody response and partial protection against Edwardsiella ictaluri in Nile tilapia

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