Systemic and intestinal porcine epidemic diarrhea virus-specific antibody response and distribution of antibody-secreting cells in experimentally infected conventional pigs

Suda, Yuto; Miyazaki, Akira; Miyazawa, Kohtaro; Shibahara, Tomoyuki; Ohashi, Seiichi

doi:10.1186/s13567-020-00880-z

Cited by 15 publications

(13 citation statements)

References 50 publications

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“…IL-4 elevation is necessary for mucosal and systemic immunity activation and IgA production 38 . Serum IgA levels have been used to predict both vaccine efficacy and the immune status of individuals or herds against enteric infectious diseases 39 , so we decided to measure total IgA in serum at BV and PV1 hypothesizing that oral vaccination would have activated the production of this antibody isotype. Indeed, serum IgA levels increased only in orally vaccinated animals (Fig.…”

Section: Resultsmentioning

confidence: 99%

Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model

et al. 2021

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Mycobacterium avium subsp. paratuberculosis (Map) causes paratuberculosis (PTB), a granulomatous enteritis in ruminants that exerts high economic impact on the dairy industry worldwide. Current vaccines have shown to be cost-effective against Map and in some cases confer beneficial non-specific effects against other pathogens suggesting the existence of trained immunity. Although Map infection is mainly transmitted by the fecal-oral route, oral vaccination has not been deeply studied. Therefore, the aim of this study was to compare the oral route with a set of mycobacterial and non-mycobacterial vaccines with a subcutaneously administered commercially available vaccine. Training effects on polymorphonuclear neutrophils (PMNs) and homologous and heterologous in vivo protection against Map were investigated in the rabbit infection model. Oral vaccination with inactivated or live vaccines was able to activate mucosal immunity as seen by elevation of serum IgA and the expression of IL4 in peripheral blood mononuclear cells (PBMCs). In addition, peripheral PMN phagocytosis against Map was enhanced by vaccination and extracellular trap release against Map and non-related pathogens was modified by both, vaccination and Map-challenge, indicating trained immunity. Finally, PBMCs from vaccinated animals stimulated in vitro with Map antigens showed a rapid innate activation cytokine profile. In conclusion, our data show that oral vaccination against PTB can stimulate neutrophil activity and both innate and adaptive immune responses that correlate with protection.

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Section: Resultsmentioning

confidence: 99%

Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model

et al. 2021

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“…Protection of neonatal piglets via lactogenic immunity depends on the trafficking of PEDV-specific IgA plasmablasts to the mammary gland and accumulation of secretory IgA (sIgA) in colostrum. Even though shnAbs are commonly used to evaluate the vaccine protective efficacy, no direct evidence supports the correlation between serum shnAbs and protective efficacy against the onsets of swine enteric virus infections ( 45 , 46 ). PEDV shnAbs might even be related to the enhancement of PEDV replication and disease symptoms in piglets ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Systemic Homologous Neutralizing Antibodies Are Inadequate for the Evaluation of Vaccine Protective Efficacy against Coinfection by High Virulent PEDV and PRRSV

Qiu

et al. 2022

Microbiol Spectr

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Porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) coinfection occurs commonly and can synergistically reduce feed intake and pig growth. Vaccination is an effective strategy utilized for PED and PRRS control, and systemic homologous neutralizing antibodies (shnAbs) in serum are commonly used for protective efficacy evaluation of PED and PRRS vaccines.

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“…Porcine epidemic diarrhoea virus (PEDV) causes severe enteric disease in pigs, which primarily enters the body via mucosal surfaces and causes disease in the small intestines [ 17 , 18 , 19 ]. To enhance the mucosal immune response to PEDV, we selected a spike (S) of PEDV as a target to express [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Immune Responses Induced by Recombinant Bacillus subtilis Expressing the PEDV Spike Protein Targeted at Microfold Cells

Lin

Mou

Zhang

et al. 2022

Veterinary Sciences

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Bacillus subtilis (B. subtilis), a probiotic bacterium and feeding additive, is widely used for heterologous antigen expression and protective immunisation. Porcine epidemic diarrhoea virus (PEDV) invades swine via mucosal tissue. To enhance the mucosal immune response to PEDV, we modified B. subtilis to express a PEDV antigen and used it as a mucosal vaccine delivery system. Initially, we constructed a recombinant B. subtilis strain (B.s-RCL) that expressed the PEDV spike protein and L-Lectin-β-GF, with the goal of inducing mucosal secretory immunoglobulin A (sIgA) and anti-PEDV serum immunoglobulin G (IgG) production, as well as to increase the number of microfold cells (M cells). Following the oral administration of B.s-RCL to mice, the small intestinal PEDV-specific sIgA expression levels significantly increased, as well as the increased number of B.s-RCL adhered to M cells. Moreover, we found that mice administered B.s-RCL exhibited markedly higher percentages of CD4+ and CD8+ T cells in the mesenteric lymph nodes and spleen compared to the control mice. Furthermore, we found that intestinal mucosa sIgA and serum anti-PEDV IgG levels were higher in mice orally immunised with B.s-RCL, suggesting that the mice could be more resistant to PEDV. In this study, we developed a novel oral vaccine to prevent porcine diarrhoea epidemics.

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Systemic and intestinal porcine epidemic diarrhea virus-specific antibody response and distribution of antibody-secreting cells in experimentally infected conventional pigs

Cited by 15 publications

References 50 publications

Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model

Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model

Systemic Homologous Neutralizing Antibodies Are Inadequate for the Evaluation of Vaccine Protective Efficacy against Coinfection by High Virulent PEDV and PRRSV

Immune Responses Induced by Recombinant Bacillus subtilis Expressing the PEDV Spike Protein Targeted at Microfold Cells

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