Systematic review on lectin-based electrochemical biosensors for clinically relevant carbohydrates and glycoconjugates

Abrantes-Coutinho, Vanessa E.; Santos, Andre; Moura, Rafael Barbosa de; Pereira-Júnior, Francisco Nascimento; Mascaro, Lúcia H.; Morais, Simone; Oliveira, Thiago M.B.F.

doi:10.1016/j.colsurfb.2021.112148

Cited by 13 publications

(9 citation statements)

References 96 publications

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“…However, lectins are able to exist in oligomer structures with multiple binding sites to simultaneously interact with different glycans of the same glycoprotein or with different arms of a branched oligosaccharide, thus achieving multivalent interaction to boost selectivity and affinity . Lectins have been utilized as capable recognition element to enable glycoprotein identification, profiling and sensing. − Tkac and co-workers have developed a series of lectin-based impedimetric biosensors for the sensitive detection and glycoprofiling of glycoproteins. , Recently, Qu and co-worker reported to modify lectin of concanavalin A (Con A) on metal–organic framework material of MIL-101(Cr)-NH 2 to afford an electrochemical impedimetric biosensing platform of Con A@MIL-101(Cr)-NH 2 for glycoprotein detection …”

Section: Receptors For Glycoproteinsmentioning

confidence: 99%

Functional Interface for Glycoprotein Sensing: Focusing on Biosensors

Qu,

2024

Langmuir

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Glycosylated proteins or glycoproteins make up a large family of glycoconjugates, and they participate in a variety of fundamental biological events. Glycoproteins have become important biomarkers in the diagnosis and treatment of a number of tumors. Biosensors are quite suitable for glycoprotein detection. The design and fabrication of a functional sensing interface play a crucial role in the biosensor construction to target glycoproteins. The functional interface, particularly receptors, typically determines the key characteristics of a biosensor, such as selectivity and sensitivity. Antibody, peptide, aptamer, boronic acid derivative, lectin, and molecularly imprinted polymer are all capable receptors for glycoprotein recognition, and each of these will be discussed. Most glycoproteins exist in low abundance, thus rendering signal amplification techniques indispensable. Nucleic acid-mediated and nanomaterial-mediated signal amplification for the detection of glycoproteins will be focused on herein. This review aims to highlight these different functional interfaces for glycoprotein sensing.

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Section: Receptors For Glycoproteinsmentioning

confidence: 99%

Functional Interface for Glycoprotein Sensing: Focusing on Biosensors

Qu,

2024

Langmuir

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Section: Introductionmentioning

confidence: 99%

“…Macro-and micro-fungi are considered important sources of lectins, i.e., proteins of nonimmune origin with carbohydrate-specific domains [1,2]. The interaction between lectins and carbohydrates or glycoconjugates occurs reversibly and maintains the protein's native conformation; this feature is among the main technological attributes of these biomolecules [2]. Despite the rich fungal diversity distributed all over the world, wild mushrooms stand out among the main sources of lectins, contributing to approximately 80% of the identified structures [1,3].…”

Section: Introductionmentioning

confidence: 99%

“…The first study on mushroom lectins dates back to 1910, when the toxicological properties of Amanita muscaria were investigated [4]. Over time, these species have captured more attention for their immeasurable nutritional and pharmacological value, showing antiproliferative, immunomodulatory, and antibacterial properties associated with lectin activity [1,2,4,5]. The presence of these macromolecules (generally 15-90 kDa molecular weight [6]) in the fruiting bodies and vegetative mycelia of several mushrooms has been reported, including those expressed by the genera Agaricus, Coprinus, Lentinus, Ganoderma, Pleurotus, Pycnoporus, Schizophyllum, Trametes, and Volvariella as representative examples [1,4,5].…”

Section: Introductionmentioning

confidence: 99%

“…There is theoretical and experimental evidence proving that ABL also recognizes lactose (β-D-galactopyranosyl-(1→4)-D-glucose) with intriguing skill [15,16]. This feature is very attractive in bioassays that require high specificity and selectivity to quantify this disaccharide in complex samples, although related studies are seldom [2]. It is worth remembering that a diet rich in lactosebased products guarantees important vitamins and minerals for our health, but people with lactose intolerance face serious gastrointestinal and extra-intestinal problems [17].…”

Section: Introductionmentioning

confidence: 99%

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Agaricus bisporus Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor

et al. 2023

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Agaricus bisporus mushroom biomass contains a lectin, ABL, with remarkable specificity for lactose biorecognition; in this work, this feature was explored to develop a photoelectrochemical biosensor. The high lectin activity found in saline extracts of this macrofungus (640 HU mL−1), even at critical pH values (4–10) and temperatures (20–100 °C), allowed its direct use as an ABL source. Theoretical and experimental evidence revealed favorable electrostatic and biocompatible conditions to immobilize ABL on a poly(methylene blue)/fluorine-doped tin oxide-coated glass platform, giving rise to the ABL/PMB/FTO biosensor. The conducting polymer added further photoactivity to the device, allowing the identification of lectin–carbohydrate interactions with even greater sensitivity. The dose–response curves studied by electrochemical impedance spectroscopy showed a sigmoidal profile that was well-fitted by Hill’s equation, expanding the working dynamic range (15–540 nmol L−1 lactose; 20.2 pmol L−1 detection limit) and avoiding undesirable sample dilution or preconcentration procedures. Under the optimized photoelectrochemical conditions, the ABL/PMB/FTO biosensor showed remarkable signal stability, accuracy, specificity, and selectivity to analyze lactose in commercial food products. This research raises interest in ABL-based biosensors and the added value of the crude Agaricus bisporus extract toward the development of greener and more sustainable biotechnological approaches.

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Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

Lapitan,

Felisilda,

Tiangco

et al. 2024

Chemistry An Asian Journal

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Pseudomonas aeruginosa is an opportunistic pathogen that infects wounds and burns and causes severe infections in humans. The high virulence, the emergence of antibiotic‐resistant strains, and the easy transmissibility of P. aeruginosa necessitate its fast detection and control. The gold standard for detecting P. aeruginosa, the plate culture method, though reliable, takes several days to complete. Therefore, developing accurate, rapid, and easy‐to‐use diagnostic tools for P. aeruginosa is highly desirable. Nanomaterial‐based biosensors are at the forefront of detecting P. aeruginosa and its secondary metabolites. This review summarises the biorecognition elements, biomarkers, immobilisation strategies, and current state‐of‐the‐art biosensors for P. aeruginosa. The review highlights the underlying principles of bioreceptor layer engineering and the design of nanomaterial‐based optical, electrochemical, mass‐based, and thermal biosensors. The advantages and disadvantages of these biosensors and their future point‐of‐care applications are also discussed.

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Systematic review on lectin-based electrochemical biosensors for clinically relevant carbohydrates and glycoconjugates

Cited by 13 publications

References 96 publications

Functional Interface for Glycoprotein Sensing: Focusing on Biosensors

Functional Interface for Glycoprotein Sensing: Focusing on Biosensors

Agaricus bisporus Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor

Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

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